Engineering bacterium based on glutamate dehydrogenase and implementation method of engineering bacterium

Abstract

The invention relates to a streptomyces griseorubens intracellular glutamate dehydrogenase coding gene and application thereof. The glutamate dehydrogenase coding gene is constructed onto an expression vector, and the recombinant expression vector is introduced into escherichia coli, so that transgenic recombinant escherichia coli capable of producing glutamate dehydrogenase in a fermentation manner is obtained. The defect that application range and effect are seriously restricted as glutamate dehydrogenase is obtained in an intracellular expression manner and expression quantity is low in the prior art is overcome, and in-vitro high expression and synthesis of glutamate dehydrogenase are realized by applying a genetic engineering technique; besides, purification of protein in a late phase is facilitated by adopting a method of adding a histidine tag in recombinant protein. The invention provides a new method for large-scale fermentation production of glutamate dehydrogenase.

Description

technical field [0001] The invention relates to a gene in the technical field of biological genetic engineering and its engineering strain, in particular to a glutamic acid dehydrogenase-based engineering strain of Streptomyces grisea and its realization method. Background technique [0002] Nitrogen is one of the most important nutrients in the process of plant growth and development. At present, the main method to maintain or increase crop yield is to apply a large amount of fertilizer. However, the extensive application of nitrogen fertilizer not only increases the production cost of farmers, but also aggravates the eutrophication of water bodies and the emission of greenhouse gases. [0003] For crops, the ability to absorb and assimilate NO 3 － , NH 4 + And different nitrogen forms such as ammoniacal N. NO 3 － When nitrogen source is used, nitrogen assimilation can be divided into three stages: (1) inorganic assimilation of nitrogen (NO 3 － -NO 2 -NH 4 + ); ...

AI Technical Summary

Problems solved by technology

At present, the main method to maintain or increase crop yield is to apply a large amount of fertilizer. However, the excessive application of nitrogen fertilizer not only increases the production cost of farmers, but also aggravates the eutrophication of water bodies and the emission of greenhouse gases.

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