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Method for synthesizing acetoin fatty acid ester in catalyzed mode through lipase

A technology of fatty acid ester and acetoin, applied in the field of biochemical industry, can solve problems such as hidden safety hazards and environmental pollution questions, and achieve the effects of low production cost, environmental friendliness and mild reaction conditions

Inactive Publication Date: 2015-04-08
CHINA UNIV OF PETROLEUM (EAST CHINA)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Raw materials such as acetic anhydride and acid chloride have great safety hazards in the process of preparation, transportation, and use, and they have also been questioned by people in terms of environmental pollution.

Method used

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  • Method for synthesizing acetoin fatty acid ester in catalyzed mode through lipase
  • Method for synthesizing acetoin fatty acid ester in catalyzed mode through lipase
  • Method for synthesizing acetoin fatty acid ester in catalyzed mode through lipase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Add 5g of hexane and 0.1798g of acetoin (ie 3-hydroxy-2-butanone) into a sealed glass screw bottle, add hexanoic acid at a molar ratio of 1:2 (acetoin:fatty acid), and keep the temperature at 50°C for 1h , and then 0.0120 g of lipase Novozyme 435 was added. The enzyme activity of the lipase is not less than 5000U / g. Shake the reaction at a constant temperature of 50°C, and the shaking speed is 150r / min. After 47 hours of reaction, a sample was taken and the concentration of the reaction product measured by gas chromatography was 9.23 g / L. The reaction product was identified as acetoin hexanoate by gas chromatography-mass spectrometry.

Embodiment 2

[0021] Same as Example 1, but replace hexanoic acid with heptanoic acid. After 47 hours of reaction, a sample was taken and the concentration of the reaction product measured by gas chromatography was 11.84 g / L. The reaction product was identified as acetoin heptanoate by gas chromatography-mass spectrometry.

Embodiment 3

[0023] Same as Example 1, but replace hexanoic acid with octanoic acid. After 47 hours of reaction, the sample was taken and the concentration of the reaction product measured by gas chromatography was 13.10 g / L. The reaction product was identified as acetoin caprylate by gas chromatography-mass spectrometry.

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Abstract

The invention discloses a method for synthesizing acetoin fatty acid ester in a catalyzed mode through lipase, and belongs to the technical field of biochemical engineering. According to the method, various acetoin fatty acid ester type compounds are synthesized by making acetoin react with fatty acid such as caproic acid, heptylic acid, octanoic acid, n-nonanoic acid, capric acid and lauric acid under the condition of 50 DEG C with hexane as reaction solvent and lipase Novozyme 435 as catalysts. The method has the advantages that the reaction condition is gentle, raw materials can be easily obtained, steps are simple, the method is environmentally friendly, the product is green and safe, and the production cost is low.

Description

technical field [0001] The invention belongs to the technical field of biochemical industry, and specifically relates to a lipase-catalyzed synthesis of acetoin hexanoate, acetoin heptanoate, acetoin octanoate, acetoin nonanoate and acetoin decanoate , The method of acetoin laurate. Background technique [0002] Acetoin fatty acid ester compounds naturally exist in some special fruits, red wine, vinegar, flowers, etc., endowing them with specific fragrance. For example, papaya contains a series of acetoin ester derivatives such as acetoin acetate, acetoin butyrate, acetoin hexanoate, acetoin octanoate, etc. A class of main aroma substances. Certain varieties of bananas also contain small amounts of acetoin acetate and acetoin butyrate. [0003] The main use of acetoin fatty acid esters is as a spice in the food and beverage industry; in addition, some acetoin esters can also be used as organic solvents for epoxy resins and vinyl resins. Due to the important application v...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/62C12P7/64
CPCC12P7/62C12P7/6436
Inventor 肖梓军侯孝元赵静宜郗丽君李静吕建仁
Owner CHINA UNIV OF PETROLEUM (EAST CHINA)