Method for curing pit mud

A technology for pit skin mud and seeds, which is applied in the preparation of alcoholic beverages, etc., can solve the problems of lack of maintenance of pit skin mud, insufficient number of functional bacteria caproic acid bacteria, and failure to consider the nutrition and metabolism of functional microorganisms, so as to avoid quality degradation, The effect of improving the quality and moisturizing of pit skin mud

Inactive Publication Date: 2015-04-15
ANHUI YINGJIA TRIBUTE WINE
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Problems solved by technology

At present, the maintenance of pit mud is mainly concentrated on the bottom mud and wall mud of the pit, but there is no effective maintenance of the pit mud, and the pit mud is only used as a seal, without considering the nutrition and metabolism of functional microorganisms. The composition and function of pit mud are simple, especially the number of caproic acid bacteria, the main functional bacteria, is insufficient

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  • Method for curing pit mud
  • Method for curing pit mud

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Embodiment 1

[0031] (1) Expanded cultivation of caproic acid bacteria seeds: ① first-level seed cultivation: After the liquid bacterial strains stored in the refrigerator were treated at 80°C for 10 minutes, they were inserted into a 100mL triangular flask with a liquid volume of 100mL according to 10% of the inoculum, and placed at 35 Cultivate at ℃ for 7 days to obtain first-grade seeds for use; ②Second-level seed cultivation: transfer the first-grade seed liquid into a 1L Erlenmeyer flask with a liquid volume of 1L according to the inoculation amount of 10%, and cultivate at 35°C for 7 days to obtain second-grade seeds for use; ③Tertiary seed cultivation: Put the secondary seed liquid into a fermenter with a liquid volume of 10L according to the inoculation amount of 10%, and cultivate it at 35°C for 7 days to obtain the third-grade seeds for use; ④Fourth-grade seed cultivation: Put the third-grade seeds According to the inoculation amount of 10%, it was put into a fermenter with a liqui...

Embodiment 2

[0046] The process flow chart of this embodiment is as figure 1 shown

[0047] A maintenance method for pit-sealed skin mud, comprising the following steps:

[0048] (1) After the five-stage expanded cultivation of caproic acid bacteria strains, the five-stage seed liquid of caproic acid bacteria is obtained;

[0049] The cultivation method of described caproic acid bacteria five-grade seed liquid is as follows:

[0050] ① After the refrigerator-preserved liquid bacteria were treated at 85°C for 10 minutes, 10% of the inoculation amount was transferred into a 100mL Erlenmeyer flask with a liquid volume of 100mL, and cultured at 37°C for 7 days to obtain a first-grade seed liquid for use;

[0051] ② Put the primary seed liquid into a 1L Erlenmeyer flask with a liquid volume of 1L according to the inoculum amount of 10%, and cultivate it at 30°C for 7 days to obtain the secondary seed liquid for use;

[0052] ③Put the secondary seed liquid into a fermenter with a liquid volum...

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Abstract

The invention discloses a method for curing pit mud. The method comprises the following steps: preparing a caproic acid bacteria five stage seed liquid; preparing a material according to a formula of caproic acid bacteria culture medium, sterilizing, cooling to 35 DEGC, adding 1-5% ethanol, and theninoculating caproic acid bacteria five stage seed liquid according to the proportion of about 10%, stirring, and conducting static cultivation at 30-37 DEG C for 5-11 d, so as to obtain a caproate bacteria culture fluid; collecting pit mud, adding a certain amount of the caproic acid bacteria culture medium, hard liquor powder, soybean powder, wheat bran, distillation tails, alcohol and water to obtain a pit mud curing mixture, uniformly stirring the mixture, and controlling the moisture content of the mixture t at 30-50%, so as to obtain treated pit mud; transferring the treated pit mud to a cement pool, sealing with film and cultivating under natural conditions for 10-40 d. The obtained curing pit mud has moist, delicate and smooth handle feel, no rough sense for rubbing, obvious caproic acid flavor; and microscopic examination shows abundant and strong Bacillus and clostridium.

Description

technical field [0001] The invention belongs to the technical field of liquor brewing, and in particular relates to a method for maintaining skin mud for cellar sealing. Background technique [0002] The production of Luzhou-flavor liquor adopts the traditional solid-state fermentation process, which strongly depends on the cellar pond, and the quality of the pit mud in the pit pond plays a very important role in the quality of Luzhou-flavor liquor. A wide variety of microbial communities with different functions that are beneficial to winemaking grow in the mud. Among them, caproic acid bacteria is a kind of Clostridium, which is a kind of cell mud functional bacteria that plays a decisive role in the fermentation of Luzhou-flavor Daqu liquor. Its quantity and function directly affect the fermentation of Luzhou-flavor Daqu liquor. Quality, because its metabolite caproic acid and fermentation product alcohol produce ethyl caproate, which is the main aroma component of Luzho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12G3/02
CPCC12G3/02
Inventor 倪永培叶玉琼李玉顶项兴本广家权叶元虎朱正祥
Owner ANHUI YINGJIA TRIBUTE WINE
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