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Method for improving polyploid poplar gamete fertility

A polyploid and gamete technology, applied in the field of plant genetics and breeding, can solve the problems of high gamete abortion rate, unfavorable sexual hybridization to produce offspring, low fertility, etc., so as to improve gamete fertility and increase new poplar germplasm creation and breeding. Efficiency, gamete fertility improvement effect

Active Publication Date: 2015-04-22
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a method for significantly improving the gamete fertility of existing polyploid plants in view of the technical problems of low gamete fertility and high gamete abortion rate in existing polyploid plants, which are unfavorable for sexual hybridization to produce offspring , to realize the sexual utilization of existing polyploid plants, especially polyploid poplar varieties, and serve for the creation and breeding of new polyploid germplasm, and the method of the present invention has a strong effect in improving the gamete fertility of polyploid poplar immediacy, effectiveness, reliability

Method used

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  • Method for improving polyploid poplar gamete fertility
  • Method for improving polyploid poplar gamete fertility
  • Method for improving polyploid poplar gamete fertility

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] 1. Hydroponic treatment of flower branches

[0037] The triploid 'Yinzhongyang' male flower branches were hydrocultured in the greenhouse, and the temperature of the greenhouse was kept at 15-25°C; the water was changed every 3 days during the hydroponics process, and the metabolites during the cultivation process were washed away under the tap water pipe . From flower branch hydroponic culture, gather male flower bud every 2-3 hours, fix at 4 ℃ with Farmer's fixative solution (95% ethanol: acetic acid=3:1), after fixing for 30 minutes, remove anther from flower bud with tweezers Take it off, place it on a glass slide, stain with 2% acetic acid magenta staining solution and pinch the anthers to release the pollen mother cells, remove large impurities, cover with a cover glass, and observe under a bright field microscope.

[0038] 2. Pollen mutagenesis treatment

[0039] When the meiosis of the pollen mother cells of 'Yinzhongyang' is observed to progress to the pachyt...

Embodiment 2

[0052] 1. Hydroponic treatment of flower branches

[0053] Same as Example 1.

[0054] 2. Pollen induction

[0055] When it was observed that the meiosis of the pollen mother cells of 'Yinzhongyang' pollen mother cells had progressed to the final change stage (bivalents were highly concentrated and dispersed to the edge of the nucleus, and the nucleolus became smaller or even disappeared), the hydroponic flower branches were immediately transferred to 5- In an environment of 10°C, to prevent it from developing too fast, a micro-syringe was used to apply 0.5% colchicine solution to the flower buds for mutagenesis treatment. The number of injections was 5 times, and the time interval between two adjacent injections was 4 hours. After the mutagenesis treatment, the flower branches were moved back to the greenhouse, and the hydroponic culture was continued until the male inflorescence matured, and the mutagenesis treatment pollen was collected.

[0056] The normally developed po...

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Abstract

The invention discloses a method for improving polyploid poplar gamete fertility, belonging to the field of plant genetics and breeding. The method comprises the following steps: observing flower bud development situations of polyploid poplars, applying a certain concentration of chemical agents for performing induced mutation treatment when observing that spore mother cells of the polyploid poplars develop to a certain period, collecting pollen after flower buds continuously develop to reach a pollen maturation period, performing pollen germination, observing the pollen germination rate, and judging the fertility level. The method disclosed by the invention can be used for providing homologous chromosomes for each chromosome of polyploidy plants so as to help completing pairing of each chromosome, the problem of unbalanced separation of the chromosomes caused in a reduction mitosis process of a polyploidy material can be solved, the gamete fertility can be significantly improved, the fertility utilization of polyploid poplar varieties can be achieved, and the breeding efficiency of new germplasms of the poplars can be improved.

Description

technical field [0001] The invention relates to a method for utilizing existing polyploid plant resources, in particular to a method for improving gamete fertility of polyploid plants, belonging to the field of plant genetics and breeding. Background technique [0002] Using polyploid materials as parents is the most direct means for plant chromosome engineering breeding and creating new polyploid germplasm. However, due to the irregular synapsis and segregation of homologous chromosomes during meiosis in polyploid plants, synaptic polyvalents are irregularly arranged in metaphase I, and chromosomes in anaphase I are unequally segregated, resulting in a large number of non-integral chromosomes. When there is a lack of a functional chromosome in the gamete, or when the coordination between genes is affected even though the functional chromosome is not missing, the gamete will inevitably be aborted, resulting in an extremely low probability of forming a normal fertile gamete. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/08
Inventor 王君田菊李代丽
Owner BEIJING FORESTRY UNIVERSITY
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