Mixed preparation of bacillus pumilus and immunopotentiator
A technology of Bacillus pumilus and an immune enhancer, which is applied in the field of microorganisms to achieve the effects of enhancing immunity, improving infection ability, increasing weight gain rate and disease resistance
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0018] Embodiment 1 Bacillus pumilus liquid fermentation
[0019] The bacillus pumilus strain is cultivated in the order of slant, liquid seed, and liquid fermentation, the slant medium is a conventional nutrient agar medium, and the liquid seed medium is a conventional nutrient broth liquid medium; liquid fermentation medium formula (W / V ): Glucose 2%, bean cake powder 8%, corn steep liquor 1%, MgSO 4 ·7H 2 O0.02%, NaCl 0.4%, KH 2 PO 4 0.1%, MnCl 2 0.04%, the fermentation temperature is 30°C, and the ventilation is cultivated for 24-48h. Obtain a high-concentration fermented broth, in which the number of viable bacteria is 10 9 -10 11 cfu / g.
[0020] It is also possible to centrifuge the fermented bacteria liquid to remove the fermented liquid to obtain wet bacteria sludge, add a heat-resistant protective agent, and then spray dry to obtain solid bacterial powder with a viable count of 10 9 -10 11 cfu / g.
Embodiment 2
[0021] Example 2 Solid state fermentation of Bacillus pumilus
[0022] The bacillus pumilus strain is cultivated in the order of slant, liquid seed and solid-state fermentation, the slant medium is conventional nutrient agar medium, and the liquid seed medium is conventional nutrient broth liquid medium. Inoculate the seed culture liquid with 5-10% inoculation amount into the solid-state fermentation medium for solid-state fermentation. The fermentation time is 24-48 hours, the fermentation temperature is 30°C, and the fermentation product is dried and pulverized to obtain the microbial agent of Bacillus pumilus . Among them, the number of live bacteria is 10 9 -10 11 cfu / g.
[0023] Wherein the solid-state fermentation medium is made up of material and water, and described material comprises rice bran 10%, bran 25%, soybean meal 32%, corn flour 30%, yeast extract 2%, NaCl 1 %, the ratio of material to water is 1:1.
Embodiment 3
[0024] The preparation of embodiment 3 mixed preparation
[0025] The described Bacillus pumilus is made into microbial inoculum through solid-state fermentation, and the number of live bacteria of Bacillus pumilus in the described microbial inoculum is 10 9 -10 11 cfu / g, 16 parts of Bacillus pumilus inoculum, 12 parts of vitamin C, 8 parts of Astragalus, 5 parts of Eucommia, 4 parts of Codonopsis, 5 parts of Angelica, 8 parts of honeysuckle, 10 parts of Bupleurum, 5 parts of Ume, 6 parts of pomegranate peel, 10 parts of schisandra. After being pulverized and passed through a 100-mesh sieve, the mixture is uniformly mixed according to the proportion by weight to obtain the mixed preparation.
PUM
| Property | Measurement | Unit |
|---|---|---|
| Viable count | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More