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Method for producing ethyl alcohol by simultaneous saccharification and fermentation on miscanthus sinensis

A technology of synchronous saccharification and fermentation and miscanthus, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of optimizing enzymatic hydrolysis efficiency and increasing production costs, and achieve low cost, less toxic substances, and equipment require simple effects

Inactive Publication Date: 2015-04-29
JIANGSU DIYIN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the current production of cellulosic ethanol will have the following problems: (1) acid-base pretreatment of lignocellulose will inevitably produce a large amount of waste liquid that is likely to cause environmental pollution, as well as toxic substances that inhibit subsequent fermentation, (2) cellulase The purchase not only greatly increases the production cost, but also makes it difficult to optimize the efficiency of enzymatic hydrolysis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Preparation of high temperature-resistant Saccharomyces cerevisiae seed liquid: Inoculate high-temperature-resistant Saccharomyces cerevisiae on YEPD plates and culture them in a constant temperature incubator at 35°C for 3 days. After colonies are formed, pick a ring (about 5×10 6 1) in 100 mL of high temperature-resistant Saccharomyces cerevisiae seed medium, cultured in a constant temperature shaker at 35°C with a rotation speed of 180 rpm for 12 hours, and then transferred the cultured primary seed liquid into 2L of secondary Grade seed cultivation tank (filling volume 1L), 35-37 ℃, rotating speed 180rpm, ventilation rate 1vvm, expand cultivation for 12h. Composition of high temperature resistant Saccharomyces cerevisiae seed medium: 5% glucose, 0.4% KH 2 PO 4 , 0.4% (NH 4 ) 2 SO 4 , 0.2% MgSO 4 , 1% yeast powder, 0.02% MnSO 4 , the pH value is 5.0;

[0018] (2) Miscanthus pretreatment: Mix Miscanthus grass with a coarse powder size of 1-2cm and tap water...

Embodiment 2

[0021] (1) Preparation of high temperature-resistant Saccharomyces cerevisiae seed liquid: Inoculate high-temperature-resistant Saccharomyces cerevisiae on YEPD plates and culture them in a constant temperature incubator at 36°C for 3 days. After colonies are formed, pick a ring (about 5×10 6 1) in 100 mL of high temperature-resistant Saccharomyces cerevisiae seed medium, and cultured in a constant temperature shaker at 36°C with a rotation speed of 180 rpm for 12 hours, and then transferred the cultivated primary seed solution into 2L of secondary Grade seed cultivation tank (filling volume 1L), 35-37 ℃, rotating speed 180rpm, ventilation rate 1vvm, expand cultivation for 12h. Composition of high temperature resistant Saccharomyces cerevisiae seed medium: 5% glucose, 0.4% KH 2 PO 4 , 0.4% (NH 4 ) 2 SO 4 , 0.2% MgSO 4 , 1% yeast powder, 0.02% MnSO 4 , the pH value is 5.0;

[0022] (2) Miscanthus pretreatment: Mix miscanthus with a coarse powder size of 1-2cm and tap wat...

Embodiment 3

[0025] (1) Preparation of high temperature-resistant Saccharomyces cerevisiae seed liquid: Inoculate high-temperature-resistant Saccharomyces cerevisiae on YEPD plates and culture them in a constant temperature incubator at 37°C for 3 days. 6 1) in 100 mL of high temperature-resistant Saccharomyces cerevisiae seed medium, and cultured in a constant temperature shaker at 37°C with a rotation speed of 180 rpm for 12 hours, and then transferred the cultivated primary seed solution into 2L of secondary Grade seed cultivation tank (filling volume 1L), 35-37 ℃, rotating speed 180rpm, ventilation rate 1vvm, expand cultivation for 12h. Composition of high temperature resistant Saccharomyces cerevisiae seed medium: 5% glucose, 0.4% KH 2 PO 4 , 0.4% (NH 4 ) 2 SO 4 , 0.2% MgSO 4 , 1% yeast powder, 0.02% MnSO 4 , the pH value is 5.0;

[0026](2) Miscanthus pretreatment: Mix Miscanthus grass with a coarse powder size of 1-2 cm and tap water at a ratio of 1.5:10 (w / v), put it into a ...

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Abstract

The invention discloses a method for producing ethyl alcohol by simultaneous saccharification and fermentation on miscanthus sinensis. The method comprises the following steps: preparing yeast seed liquid; pretreating miscanthus sinensis; carrying out simultaneous saccharification and fermentation. The method has the advantages that in a pollution-free hydrothermal pretreatment method for a miscanthus sinensis material, no compounds such as acid and alkali are added, so that the quantity of toxic substances is small, and the equipment requirement is simple; meanwhile, by cellulase produced by the miscanthus sinensis serving as a substrate, the hydrolysis efficiency is high and the cost is low.

Description

technical field [0001] The invention relates to a method for producing fuel ethanol, in particular to a method for producing ethanol by using Miscanthus synchronous saccharification and fermentation. Background technique [0002] Ethanol is an important industrial raw material, which is widely used in food, chemical industry, medical and health and military fields. The current raw materials for ethanol production are mainly food and economic crops such as starch and sugar, including corn, wheat and sugarcane; in today's As the world faces the severe situation of increasing energy demand and worsening environmental pollution, ethanol has increasingly become a liquid fuel to supplement gasoline. It is obviously unsustainable to use food or cash crops to solve the energy problem. [0003] Cellulosic ethanol uses agricultural waste or second-generation energy crops produced on marginal land as raw materials. It is regarded as an ideal liquid fuel that can partially replace gaso...

Claims

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Application Information

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IPC IPC(8): C12P7/10C12R1/865
CPCC12P7/10Y02E50/10
Inventor 杨文慧牛堃
Owner JIANGSU DIYIN BIOTECH
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