Preparation method of gel scaffold for repairing articular cartilage damage

A technology for cartilage damage and joint repair, applied in medical science, prosthesis, etc., can solve the problems of growth factor immunogenicity that cannot be used in vivo, safety tumorigenicity of gene transfection technology, and unfavorable differentiation of mesenchymal stem cells. , to achieve the effect of being beneficial to clinical application, beneficial to the repair of cartilage damage, and shortening of light intensity and light time.

Active Publication Date: 2016-05-04
NANJING ZHENQUAN MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods have various limitations: growth factors cannot be used in vivo due to immunogenicity, safety and tumorigenicity problems exist in gene transfection technology, and it is difficult to integrate tissue-engineered cartilage implants with original tissues, etc.
[0005] At present, most of the scaffold materials used in research and application are PLGA, PGA, collagen, fibrin, etc. These scaffold materials are characterized by being able to induce cells to produce a large amount of type I collagen instead of type II collagen, which is not conducive to mesenchymal Stem cells differentiate into chondrocytes
In the research field of drug sustained-release stents, most of the drugs used for sustained release are growth factors, which are expensive and immunogenic, and their application in vivo may bring the risk of animal-derived diseases

Method used

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  • Preparation method of gel scaffold for repairing articular cartilage damage
  • Preparation method of gel scaffold for repairing articular cartilage damage
  • Preparation method of gel scaffold for repairing articular cartilage damage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] This embodiment is a preparation method of a gel scaffold for repairing articular cartilage damage, which specifically includes the following steps:

[0072] (1) Modification of sodium hyaluronate;

[0073] (2) Dissolve KGN PLGA nanoparticles in step (1) modified sodium hyaluronate at a concentration of 10um / L, then add photoinitiator Irgacure 2595 to obtain sustained-release small molecule compound-sodium hyaluronate gel In the composite system, the amount of photoinitiator added is 1 / 9 of the small molecule compound by weight ratio;

[0074] (3) Inject / fill the sustained-release small molecule compound-sodium hyaluronate gel complex system into the joint cartilage damage to be repaired and irradiated by a UV point light source, 20μW / cm 2 The UV point light source is irradiated for 20S to obtain a gel scaffold for repairing articular cartilage damage.

[0075] The preparation method of the KGN PLGA nanoparticles includes the following steps:

[0076] (1) Dissolve 45 mg of PLGA ...

Embodiment 2

[0081] This embodiment is a preparation method of a gel scaffold for repairing articular cartilage damage, which specifically includes the following steps:

[0082] (1) Modification of sodium hyaluronate;

[0083] (2) Dissolve KGN PLGA nanoparticles in step (1) modified sodium hyaluronate at a concentration of 2um / L, and then add photoinitiator Irgacure 2595 to obtain a sustained-release small molecule compound-sodium hyaluronate gel In the composite system, the amount of photoinitiator added is 1 / 10 of the small molecule compound by weight ratio;

[0084] (3) Injecting / filling the sustained-release small molecule compound-sodium hyaluronate gel complex system into the joint cartilage damage to be repaired and irradiated by a UV point light source, 10μW / cm 2 The UV point light source is irradiated for 40S to obtain a gel scaffold for repairing articular cartilage damage.

[0085] The preparation method of the KGN PLGA nanoparticles includes the following steps:

[0086] (1) Dissolve PL...

Embodiment 3

[0091] This embodiment is a preparation method of a gel scaffold for repairing articular cartilage damage, which specifically includes the following steps:

[0092] (1) Modification of sodium hyaluronate;

[0093] (2) Dissolve KGN PLGA nanoparticles in step (1) modified sodium hyaluronate at a concentration of 60um / L, and then add photoinitiator Irgacure 2595 to obtain a sustained-release small molecule compound-sodium hyaluronate gel For the composite system, according to the weight ratio, the amount of photoinitiator added is 1 / 8 of the small molecule compound;

[0094] (3) Injecting / filling the sustained-release small molecule compound-sodium hyaluronate gel complex system into the joint cartilage damage to be repaired and irradiated by a UV point light source, 6μW / cm 2 The UV point light source is irradiated for 1 min to obtain a gel scaffold for repairing articular cartilage damage.

[0095] The preparation method of the KGN PLGA nanoparticles includes the following steps:

[0096...

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Abstract

The invention relates to the fields of medicines and medical instruments, and particularly relates to a preparation method for a gel scaffold for repairing articular cartilage injuries. The preparation method specifically comprises the following steps: (1) modifying sodium hyaluronate; (2) dissolving PLGA nano-particles of a controlled-release small molecular compound in the sodium hyaluronate modified in the step (1), and then adding with a photoinitiator to obtain a controlled-release small molecular compound-sodium hyaluronate gel compound system; and (3) injecting / filling the controlled-release small molecular compound-sodium hyaluronate gel compound system to the articular cartilage injuries to be repaired for being irradiated by an ultraviolet point light source, so as to obtain the gel scaffold for repairing articular cartilage injuries. The gel scaffold provided by the invention is high in plasticity, and capable of being randomly shaped according to the shapes of cartilage defects; harmful substances are not introduced in the whole process, and the gel scaffold can be used in vivo.

Description

Technical field [0001] The invention relates to the field of medicines and medical devices (interventional medical devices), in particular to a preparation method of a gel stent for repairing articular cartilage damage. Background technique [0002] As our country enters an aging society, more and more patients have knee cartilage injuries. There is currently no good treatment for such diseases. At present, the commonly used subchondral bone perforation, osteochondral transplantation and auto-chondrocyte transplantation can only partially relieve symptoms and cannot achieve the purpose of reconstructing cartilage defects. The main component of repair tissue obtained by using these methods is type I collagen, and Compared with the original type II collagen of hyaline cartilage, the biological characteristics are far different. [0003] The basic principle of regenerating and repairing the cartilage defect and reconstructing the cartilage defect is: recruiting oneself or introducin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/52A61L27/20A61L27/18A61L27/54
Inventor 顾臻史冬泉
Owner NANJING ZHENQUAN MEDICAL TECH CO LTD
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