Method for extracting coenzyme Q10 from fermentation mycelia

An extraction method and a technology of fermenting bacteria, which are applied in the field of extracting coenzyme Q10 in fermented bacteria, can solve the problems of low yield of organic solvent extraction, large investment in extraction equipment, and decline in product quality, and meet the requirements of large solvent diffusion coefficient and equipment requirements. Low, less solvent loss effect

Active Publication Date: 2015-05-06
INNER MONGOLIA KINGDOMWAY PHARMA LTD +1
View PDF4 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] For the problems existing in the above-mentioned prior art, the purpose of the present invention is to provide a kind of method utilizing subcritical fluid to extract coenzyme Q10, overc

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for extracting coenzyme Q10 from fermentation mycelia
  • Method for extracting coenzyme Q10 from fermentation mycelia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1. utilize subcritical propane fluid to extract coenzyme Q10

[0032]Weigh 500g of coenzyme Q10 bacterial sample (content 24.3mg / g), pulverize it with a pulverizer, the particle size is controlled between 10 and 120 mesh, put it in a closed extraction tank, close the feed valve, pump to vacuum, press the bacterial cell Weight (g) / solvent volume (ml) = 1 / 3 Inject propane solvent, stir and mix and extract 3 times under the conditions of temperature 20-25°C and pressure 0.85-0.88MPa, each time 30min; after the extraction is completed, transfer out For the extract in the extraction tank, open the outlet valve of the extraction tank for desolvation, heat the extraction tank with hot water, and stir, the heating temperature is 45-50 ° C, the stirring speed is 30 rpm, and the pressure of the extraction tank is reduced to 0 MPa , Start the vacuum pump for negative pressure evaporation until the pressure drops below -0.085MPa, stop the precipitation, open the nitrogen...

Embodiment 2

[0033] Embodiment 2. Utilize subcritical butane fluid to extract coenzyme Q10

[0034] Weigh 500g of coenzyme Q10 bacterial sample (content 24.3mg / g), pulverize it with a pulverizer, the particle size is controlled between 10 and 120 mesh, put it in a closed extraction tank, close the feed valve, pump to vacuum, press the bacterial cell Weight (g) / solvent volume (ml)=1 / 3 Inject the solvent, stir and mix and extract 3 times at a temperature of 25-30°C and a pressure of 0.30-0.35MPa, each time 20min; The method in 1 removes the solvent, takes out the sample, collects and obtains 25.30 g of coenzyme Q10 extract, the content of coenzyme Q10 in the extract is 47.2%, and the extraction yield is 98.3%.

Embodiment 3

[0035] Example 3. Utilize subcritical dimethyl ether fluid to extract coenzyme Q10

[0036] Weigh 500g of coenzyme Q10 bacterial sample (content 24.3mg / g), pulverize it with a pulverizer, the particle size is controlled between 10 and 120 mesh, put it in a closed extraction tank, close the feed valve, pump to vacuum, press the bacterial cell Weight (g) / solvent volume (ml)=1 / 3 Inject dimethyl ether solvent, stir and mix and extract 3 times under the conditions of temperature 25-30°C and pressure 0.48-0.52MPa, each time 20min; after the extraction, Remove the solvent according to the method in Example 1, take out the sample, collect and obtain 25.28 g of coenzyme Q10 extract, the content of coenzyme Q10 in the extract is 46.8%, and the extraction yield is 97.4%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a method for extracting a coenzyme Q10 from fermentation mycelia. The method comprises the following steps: filtrating and drying coenzyme Q10 fermented liquid so as to obtain mycelia, granulating the mycelia into 10-120-mesh granules, putting the granules into an extracting tank, carrying out subcritical extraction on the bacteria by taking subcritical fluid as an extraction solvent, then filtering so as to obtain extraction liquid and fungi residues, feeding the extraction liquid into an evaporation system, desolventizing the extraction liquid under a reduced pressure condition so as to obtain an coenzyme Q10 extract, and carrying out subsequent purification treatment on the coenzyme Q10 extract so as to obtain an coenzyme Q10 finished product. Because the subcritical fluid is adopted for extracting coenzymes Q10, the method disclosed by the invention is high in extraction yield, low in solvent loss, good in quality of products, low in operation cost, relatively short in production cycle, and large in relative yield, therefore, the method is a high-efficiency, low-consumption, environment-friendly and clean production technology of coenzymes Q10.

Description

technical field [0001] The invention relates to a method for extracting coenzyme Q10 in fermented bacteria, and relates to the field of microorganism extraction. Background technique [0002] Coenzyme Q10, English name Coenzyme Q10, referred to as CoQ10. It is a vitamin-like substance widely distributed in the human body, especially in the heart with the highest content. It is the only natural nutrient on the market that has the functions of activating cellular respiration and anti-free radical oxidation. It is a powerful antioxidant when used alone or in combination with vitamin E. As a metabolic activator, it exists in the mitochondrial inner membrane of cells and generates cellular power through specific cellular metabolism. At the same time, it is also a vitamin-like nutrient substance. After consumption, it can activate cellular respiration, accelerate the production of high-energy ATP, strengthen myocardial metabolism, and improve heart beating efficiency. It can i...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07C50/28C07C46/10
CPCC07C46/10C07C50/28
Inventor 王炳荣蒋四富张斌焉世杰塔娜沈初亮
Owner INNER MONGOLIA KINGDOMWAY PHARMA LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap