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Method for efficiently extracting polysaccharide of lycium barbarum leaves

A technology of wolfberry leaves and polysaccharides, which is applied in the field of polysaccharide extraction, can solve the problems of long extraction time, limited extraction range, and high operating costs, and achieves benefits for modern industrial production and continuous production, saving raw materials and time, and low operating costs Effect

Active Publication Date: 2015-05-06
森淼科技集团股份有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, many of these mainstream extraction methods have obvious disadvantages, such as: when extracting polysaccharides by water extraction, the polysaccharide yield is often lower than 5%, and the extraction takes a long time, and the impurities contained in the extract are relatively large. This limits the application of this method in practical applications, especially in large-scale production
Similarly, the acid extraction method will cause the breakage of glycosidic bonds, and the alkaline extraction method will cause the flavor and color of the extract to change. Although the supercritical fluid extraction method can improve the yield and efficiency of polysaccharides, due to its relatively complicated equipment and operating costs High, limited extraction range and other shortcomings, still can not be used on a large scale

Method used

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  • Method for efficiently extracting polysaccharide of lycium barbarum leaves
  • Method for efficiently extracting polysaccharide of lycium barbarum leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Wash wolfberry leaves (Foliis medlar), dry at 50°C for 7 hours, grind and pass through a 60-mesh sieve.

[0028] 2. Accurately weigh 50g of the sieved powder into a beaker, add 300ml of ether, stir for 20min and then suction filter.

[0029] 3. Move the filter cake into a beaker, add 2.5L of distilled water, put it in a sterilizing pot, treat it under the conditions of 121°C and 0.25MPa for 60 minutes, and filter it with suction. Extract the filter cake twice under the same conditions, and combine the filtrates.

[0030] 4. Concentrate the filtrate to 1 / 10 of the original volume in a vacuum distillation device, add 2 times the volume of savage solution (chloroform:n-butanol=4:1, volume ratio), shake it for 20 minutes and then place it in a centrifuge at 1500r / Centrifuge for 20 minutes, remove the intermediate protein layer, add absolute ethanol until the volume fraction of ethanol reaches 80%, and let stand for 6 hours to precipitate.

[0031] 5. The filter cake ob...

Embodiment 2

[0033] 1. Wash wolfberry leaves (Foliis medlar), dry at 40-60°C for 6-8 hours, grind and pass through a 60-mesh sieve.

[0034] 2. Accurately weigh 50g of the sieved powder into a beaker, add 300ml of ether and stir for 20min, then suction filter.

[0035] 3. Transfer the filter cake into a beaker, add 2.0L of distilled water, put it in a sterilizing pot, treat it at 121°C and 0.25 MPa for 60 minutes, and filter it with suction. Extract the filter cake twice under the same conditions, and combine the filtrates.

[0036] 4. Concentrate the filtrate to 1 / 10 of the original volume in a vacuum distillation device, add 2 times the volume of savage (chloroform: n-butanol = 4:1, volume ratio) solution, shake (no special requirements) for 20 minutes and then in Centrifuge at 1500r / min for 20min in a centrifuge, remove the intermediate protein layer, add absolute ethanol until the volume fraction of ethanol reaches 80%, and let stand for 4-8h to precipitate.

[0037] 5. The filter cak...

Embodiment 3

[0039] 1. Wash wolfberry leaves (Foliis medlar), dry at 40-60°C for 6-8 hours, grind and pass through a 60-mesh sieve.

[0040] 2. Accurately weigh 50g of the sieved powder into a beaker, add 300ml of diethyl ether and stir for 20min, then suction filter.

[0041] 3. Move the filter cake into a beaker, add 1.5L of distilled water, put it in a sterilizing pot, treat it at 121°C and 0.25 MPa for 60 minutes, and filter it with suction. Extract the filter cake twice under the same conditions, and combine the filtrates.

[0042]4. Concentrate the filtrate to 1 / 10 of the original volume in a vacuum distillation device, add 2 times the volume of savage solution (chloroform: n-butanol = 4:1, volume ratio), shake (no special requirements) for 20 minutes and then in Centrifuge at 1500r / min for 20min in a centrifuge, remove the intermediate protein layer, add absolute ethanol until the volume fraction of ethanol reaches 80%, and let stand for 4-8h to precipitate.

[0043] 5. The filter ...

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Abstract

The invention relates to a method for extracting polysaccharide from lycium barbarum leaves and particularly relates to a method for efficiently extracting polysaccharide of lycium barbarum leaves. The method is characterized by comprising the following steps: (1) treating raw materials: drying lycium barbarum leaves and then crushing and sieving by a 40-80-mesh sieve; (2) adding petroleum ether into powder obtained, vibrating for 20-30 minutes, carrying out suction filtration, and drying for 1-3 hours in a drying box at a reduced box at 40-60 DEG C so as to obtain lycium barbarum leaf powder without fat; and (3) adding distilled water into the obtained lycium barbarum leaf powder, controlling the material-water ratio at 1g / 30-50ml, extracting, after suction filtration, extracting filter residues twice by virtue of the method, and mixing the extraction liquids obtained three times. The invention provides the method for efficiently extracting polysaccharide of lycium barbarum leaves. Based on a water extraction method in addition to a high-temperature high-pressure condition, the dissolution speed and the dissolution rate of polysaccharide of cells of the lycium barbarum leaves are increased. The yield is over 2 times that of other methods, and the raw materials are saved and the time is shortened.

Description

technical field [0001] The invention relates to a method for extracting polysaccharides from wolfberry leaves, in particular to a method for efficiently extracting polysaccharides from wolfberry leaves. Background technique [0002] Polysaccharides, as a natural product with various health effects such as anti-virus, anti-cancer, hypoglycemic, immune system regulation, and help regeneration and repair of body tissue structure, play an important role in the research and development of modern health food. Among the existing polysaccharide extraction processes, the more common methods include water extraction and alcohol precipitation, acid extraction, alkali extraction, supercritical fluid extraction, enzymatic hydrolysis, and physical assisted extraction. [0003] However, many of these mainstream extraction methods have obvious disadvantages, such as: when extracting polysaccharides by water extraction, the polysaccharide yield is often lower than 5%, and the extraction take...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 王立英范俊峰徐士梅金明王储
Owner 森淼科技集团股份有限公司
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