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Method for culturing salt-tolerant aerobic activated sludge

A technology of activated sludge and oxygen activity, applied in chemical instruments and methods, water/sludge/sewage treatment, biological water/sewage treatment, etc.

Active Publication Date: 2015-05-13
BLUESTAR LEHIGH ENG INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The purpose of the invention is to propose a simple and easy-to-implement method for cultivating salt-tolerant aerobic activated sludge for the defects and limitations of existing high-salt wastewater biochemical treatment methods, and the cultivated salt-tolerant aerobic activated sludge not only It has good salt tolerance, resistance to salinity impact and organic matter degradation ability, and can stably and efficiently degrade organic matter in high-salt wastewater without being inhibited by salt concentration in a high-salt environment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1, a kind of method for cultivating salt-tolerant aerobic activated sludge comprises two stages of enrichment cultivation and stuffy exposure, wherein:

[0054] (1) Enrichment culture takes salt-tolerant microorganisms in high-salt environment samples as enrichment culture objects to obtain a mixture containing salt-tolerant microorganisms;

[0055] (2) Suffocation is to continue to cultivate the mixed solution obtained from enrichment culture under aeration conditions, so as to promote the formation of bacterial micelles of salt-tolerant microorganisms, and finally cultivate activated sludge that can separate mud and water, that is, salt-tolerant aerobic activity sludge;

[0056] (3) The whole culture process is a mixed culture of salt-tolerant microorganisms, the concentration of inorganic salt in the culture system is 1-25%, and the culture is carried out in an open environment.

[0057] The sources of the high-salt environment samples are but not limited...

Embodiment 2

[0065] Embodiment 2, a method for cultivating salt-tolerant aerobic activated sludge, including two stages of enrichment culture and vexed exposure, wherein:

[0066] The high-salt environment samples were taken from offshore seabed mud and stored in a refrigerator at 4°C after retrieval.

[0067] Nutrient elements: (NH 4 ) 2 SO 4 0.1~1.2g / L, K 2 HPO 4 0.2~1.4g / L, KH 2 PO 4 0.05~0.6g / L, MgSO 4 0.05~0.15g / L, CaCl 2 0.05~0.2g / L.

[0068] Trace elements: MnCl 2 0.000001~0.00004g / L,H 3 BO 3 0.00001~0.0004g / L, CoCl 2 0.00001~0.0002g / L, CuCl 2 0.000005~0.0002g / L, NiCl 2 0.000005~0.0002g / L, Na 2 MoO4 0.00001~0.0003g / L, ZnCl 2 0.000005~0.0002g / L, FeSO 4 0.0001~0.0002g / L, EDTA 0.003~0.006g / L.

[0069] Organic carbon sources: methanol, phenol. Bio-extracted nutrients: beef extract. Enrichment medium: nutrients, trace elements, methanol, phenol, beef extract, 3-6% NaCl or Na 2 SO 4 , distilled water preparation, pH 6.5 ~ 8.5. Inorganic salt medium: nutrie...

Embodiment 3

[0075] Embodiment 3, a kind of method for cultivating salt-tolerant aerobic activated sludge, comprises two stages of enrichment cultivation and stuffy exposure, wherein:

[0076] The high-salt environment samples were taken from offshore seabed mud and stored in a refrigerator at 4°C after retrieval.

[0077] Nutrient elements: (NH 4 ) 2 SO 4 0.1~1.2g / L, K 2 HPO 4 0.2~1.4g / L, KH 2 PO 4 0.05~0.6g / L, MgSO 4 0.05~0.15g / L, CaCl 2 0.05~0.20g / L.

[0078] Organic carbon sources: glucose, phenol. Bio-extracted nutrients: peptone. Enrichment medium: nutrient elements, methanol, phenol, peptone, 13-17% NaCl, prepared with tap water, pH 6.5-8.5. Inorganic salt medium: nutrients, methanol, phenol, 13-17% NaCl, prepared from tap water, pH 6.5-8.5.

[0079] Training steps:

[0080] (1) Enrichment culture: In a 250mL Erlenmeyer flask, add 50mL enrichment medium, including glucose 50-100mg / L, phenol 5-20mg / L, beef extract 100-500mg / L, peptone 100-500mg / L, and then Add abou...

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Abstract

The invention relates to a method for culturing salt-tolerant aerobic activated sludge. The method comprises the steps of enrichment culture and wastewater entrance-free aeration; the enrichment culture is characterized in that a salt tolerant microbiota in a high salinity environment sample is cultured as an enrichment culture object to obtain a salt tolerant microbiota-containing mixed solution; wastewater entrance-free aeration is characterized in that the mixed solution obtained through the enrichment culture is continuously cultured under aeration conditions to promote the salt tolerant microbiota form a zoogloea, and the zoogloea is cultured to obtain sludge-water separation activated sludge which is the salt-tolerant aerobic activated sludge; and the whole culture process is salt tolerant microbiota mixed culture, the concentration of inorganic salts in the above culture system is 1-25%, and culture is carried out in open environment. The salt-tolerant aerobic activated sludge cultured through the method has wide salt tolerance range and strong salinity impact resistance, and can be used in the biochemical treatment of high salinity organic wastewater with the total salinity of 1-25%.

Description

technical field [0001] The invention belongs to the technical field of wastewater biochemical treatment, and in particular relates to a method for cultivating salt-tolerant aerobic activated sludge required for biochemical treatment of high-salt organic wastewater. Background technique [0002] High-salt wastewater refers to wastewater with a mass fraction of inorganic salts greater than 1%. Its sources include: (1) seawater utilization: wastewater generated from seawater processing, mariculture, and seawater substitute discharge; (2) industrial wastewater: food, petroleum, chemical industry , printing and dyeing, papermaking, leather, pharmaceutical, fermentation and other industries widely produce high-salt and high-concentration organic wastewater; (3) Others: ship ballast water, salt water generated by minimizing waste water (garbage leachate, reverse osmosis concentrated drainage), Domestic sewage from large ships. The high-salt wastewater has a large amount and a wide...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/12
CPCC02F3/121C02F2103/36Y02W10/10
Inventor 徐军王开春董自斌李学字云干杨志林刘志奎李坤张彬彬赵焰
Owner BLUESTAR LEHIGH ENG INST CO LTD
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