Culture collection method for fungi mycelium

Abstract

The invention discloses a culture collection method for fungi mycelium. The culture collection method comprises the following steps: tiling glass paper on a culture medium of fungi growth, inoculating a fungal strain block on the glass paper in a sterile manner, and collecting fungi mycelium when the mycelia are grown in the culture medium. An applicant accidently finds that during the culture of fungi mycelium, the glass paper is taken as an isolating film, so that the fungal strain block is absorbed to the culture medium to nutritively and normally grow due to the gas permeability of the glass paper, the transparency of the glass paper is convenient for observing the appearances of the mycelia, and the mycelia and the culture medium are physically isolated to conveniently collect the mycelia without impurities. According to lots of experiments, the mycelia obtained by the culture collection method are suitable for extraction of total DNA, RNA or proteins and the like. The culture collection method is simple to operate, short in period and low in contamination rate; the obtained mycelia are pure and impurity-free; the glass paper is strong in easy decomposability, low in cost, free of environmental pollution and worthy of being popularized and used on a large scale.

Description

technical field [0001] The invention belongs to the field of microorganisms and relates to a method for cultivating and collecting fungal mycelia. Background technique [0002] In the research of extracting total DNA, RNA and protein of fungal mycelium or fungal fermentation formula and parameter exploration, researchers need to culture and proliferate mycelium. At present, the method of cultivating and proliferating mycelia usually adopts liquid fermentation culture and then separates the mycelia from the fermentation broth, or directly uses a solid medium such as a test tube slope or a flat plate to cultivate bacteria and then scrapes the surface mycelium. [0003] At present, for the mycelia cultured in liquid, high-speed centrifugation and filtration are mostly used to separate the mycelium from the fermentation broth. However, both separation methods have certain problems. For the high-speed centrifugation method, on the one hand, because the mycelium of some fungal s...

AI Technical Summary

Problems solved by technology

However, both separation methods have certain problems

For the high-speed centrifugation method, on the one hand, because the mycelium of some fungal strains in liquid culture is relatively viscous and in the state of large particles of suspension, it is difficult to completely compact the mycelium even if a high centrifugal speed is used. Settling to the bottom of the centrifuge tube, the separation effect is poor; on the other hand, the water inside the mycelium ball particles is also difficult to remove by centrifugation, and there are also problems such as high cost of the centrifuge, cumbersome operation and time-consuming

For the filtration method, currently include the method of vacuum filtration using filter paper and the ordinary gauze filtration method. For the filter paper vacuum filtration method, on the one hand, there are also high and viscous fermented liquids of certain fungal strains, which are difficult to obtain. The problem of drier mycelium, on the other hand, for such a fermentation liquid, the micropores of the filter paper are easily blocked by the fermentation liquid, so it is difficult to filter in the future, and the filter paper must be replaced continuously, and the filter paper is easy to be scratched when it is wet , so the mycelium adsorbed on the filter paper is also difficult to remove completely; for the gauze filtration method, since the gauze is easy to stain the mycelium, it is easy to cause cross-contamination of the mycelium between different samples in the case of a large number of samples. Repeated use, thereby causing unnecessary waste, and when using gauze to filter, the mycelia will easily leak out if the number of gauze layers is small, and the mycelia will easily remain between each layer of gauze and in the holes of the gauze if the number of layers is large, which will easily cause waste and waste of samples. data error

[0004] For the method of scraping the surface mycelium after culturing mycelium on a test tube slope or flat plate, on the one hand, the amount of mycelium is too small to meet the experimental requirements; It is not only cumbersome to operate, but also affects the development of subsequent molecular biology experiments and the accuracy of experimental results.

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