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Bifunctional strain capable of degrading cellulose and starch as well as separating and screening method and application of bifunctional strain

A technology for degrading cellulose, separating and screening, applied to a bifunctional bacterial strain for degrading cellulose and starch and its separation, screening and application fields, which can solve the problems of long cycle time and slow degradation of domestic waste, and achieve low cost and resource saving , high cellulose and amylase activity

Inactive Publication Date: 2015-05-13
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problem of slow degradation and long cycle of domestic garbage caused by low temperature restrictions in the northern severe cold region, and the problem of antagonism and mutual inhibition of enzyme activity when two types of functional strains are screened out respectively, and provided A bifunctional strain for separating and screening degrading cellulose and starch and the medium used therefor

Method used

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  • Bifunctional strain capable of degrading cellulose and starch as well as separating and screening method and application of bifunctional strain
  • Bifunctional strain capable of degrading cellulose and starch as well as separating and screening method and application of bifunctional strain

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specific Embodiment approach 1

[0016] Specific embodiment 1: In this embodiment, a cellulose- and starch-degrading bifunctional bacterial strain is isolated and screened, which is Flavobacterium sp. FL-1, and is preserved in the China Common Microorganism Culture Collection Management Center, and the preservation address is Beijing No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, the city, the preservation date is January 4, 2015, and the preservation number is CGMCC No.10272.

[0017] The Flavobacterium sp. FL-1 of this embodiment is Gram-negative, straight rod-shaped, round-ended, does not form endospores, and is strictly aerobic.

[0018] Table 1 shows the physiological and biochemical indicators of Flavobacterium sp. FL-1 in this embodiment.

[0019] Table 1 Physiological and biochemical indicators of Flavobacterium sp. FL-1

[0020]

[0021]

specific Embodiment approach 2

[0022] Specific embodiment two: present embodiment a kind of method for separation and screening of degrading cellulose and starch bifunctional bacterial strain, it is to realize according to the following steps:

[0023] 1. Dilute the collected domestic waste soil samples with sterile water under aerobic conditions;

[0024] 2. Spread the diluted bacterial solution on the surface of the solid medium, and then incubate at a constant temperature of 5-15°C for 3-10 days;

[0025] 3. Pick a single colony in the liquid medium, and then culture it at a constant temperature of 10°C for 5 to 7 days;

[0026] 4. Repeat the operation of step 3 until the pure bacterial strain is isolated to obtain the dual-function bacterial strain for degrading cellulose and starch;

[0027] Wherein, the solid medium is cellulose solid medium and starch solid medium; the liquid medium is cellulose liquid medium and starch liquid medium.

[0028] This embodiment isolates and screens the cellulose-degr...

specific Embodiment approach 3

[0030] Specific embodiment three: the difference between this embodiment and specific embodiment two is: the cellulose liquid culture medium is made of 15.0g sodium carboxymethyl cellulose, 1.4g (NH 4 ) 2 SO 4 , 0.5g of MgSO 4 ·7H 2 O, 2.0 g of K 2 HPO 4 , 0.3g of CaCl 2 , 5mg of FeSO 4 ·7H 2 O, 1.6 mg of MnSO 4 , 1.7ng of ZnCl 2 , 1.7mg of CoCl 2 Made with 1000mL distilled water, adjust the pH to 7.0;

[0031] The starch liquid medium is made of 10g of soluble starch, 3g of beef extract, 10g of peptone, 5g of NaCl and 1000mL of distilled water, and adjusts the pH to 7.0;

[0032] The cellulose solid medium and the starch solid medium are made by adding 20 g of agar respectively on the basis of the above components. Other steps and parameters are the same as in the second embodiment.

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Abstract

The invention discloses a bifunctional strain capable of degrading cellulose and starch as well as a separating and screening method and application of the bifunctional strain and relates to a bifunctional strain as well as a separating and screening method and application thereof, for solving the problem of low degradation speed of household garbage caused by the low temperature limitation. The strain is flavobacterium sp. FL-1, and is collected in China General Microbiological Culture Collection Center (CGMCC) on January 4, 2015 with a collection number of CGMCC No. 10272. The separating method comprises the following steps of firstly, preparing a doubling dilution liquid with different concentrations; secondly, enabling colonies to appear in the plate; thirdly, carrying out low-temperature constant-temperature incubation; and fourthly, separating and purifying until a pure strain is obtained. The bifunctional strain can simultaneously degrade cellulose and amylase and has the characteristic of high degradation efficiency of cellulose and starch. A culture medium used in screening is strong in pertinency. The method has the advantages of short process time, resource conservation, low cost and simple device.

Description

technical field [0001] The invention relates to a bifunctional bacterial strain degrading cellulose and starch, its separation and screening method and application. Background technique [0002] With the improvement of human material living standards, organic domestic waste in rural areas has become a serious social problem. According to relevant statistical data, the average annual growth rate of garbage in my country has reached more than 10%, becoming one of the countries with the most serious garbage siege in the world, especially in severe cold areas, due to low temperature restrictions, the degradation rate of domestic garbage is extremely slow, and effective control is urgently needed The problem of environmental pollution caused by domestic waste under low temperature conditions calls for comprehensive and effective treatment of domestic waste. [0003] Due to the low temperature in autumn and winter in northern my country, the degradation rate of domestic waste is s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/20
CPCC12N1/20C12N1/205C12R2001/20
Inventor 李大鹏李春艳成小松侯宁冯露成毅柳宏原刘科然曹会鸣黄馨凝
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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