A method of synthesizing palladium nanometer particles by enterococcus faecalis
A technology of palladium nanoparticles and Enterococcus faecalis, applied in the field of wastewater treatment, can solve the problems of high cost and large secondary pollution
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Embodiment 1
[0034] Enterococcus faecalis Z5 cultured to the logarithmic growth phase was collected by centrifugation, washed three times with 20 mM MPOS buffer to remove possible residual medium, and then prepared a bacterial suspension for use. Resuspend the bacteria in MOPS buffer, measure the OD value, in order to determine the dry weight of the cells and the OD of the bacterial solution 600 For correlation between bacteria, take 2ml of bacterial culture solution, centrifuge at 8000rpm, and collect bacterial cells for 5 minutes. Place the bacterial cells in the centrifuge tube (weighed first) in an oven to dry at 60° C. for 5-6 hours. Weigh after drying, remove the initial weight of the centrifuge tube, and calculate the dry cell weight (mg) and unit culture medium (mg / mL culture medium) in the culture medium taken. Cell dry weight and bacterial solution OD 600 the correlation between figure 1 shown.
Embodiment 2
[0036] Take 1mL of the bacterial suspension and add it to a 50mL Erlenmeyer flask, add 3.333mL of 6mM Na 2 PdCl 4 solution, add water to make the system 10mL. The final palladium ion concentration was 2 mM, and the Erlenmeyer flask was placed at 40°C for 6 hours of adsorption, and samples were taken at different time intervals to measure the concentration of remaining palladium ions in the solution. The solution changed from the previous yellow color to white and brown granular precipitates. The characterization results of bacterial adsorption were verified by ultraviolet-visible spectroscopy and transmission electron microscopy as follows: Figure 2-5 shown.
Embodiment 3
[0038] Use 12 g / L dry weight of bacteria (take the volume of the solution and de-centrifuge accordingly), add 20 mM MOPS buffer in 1 mL into a 50 mL Erlenmeyer flask, add 3.333 mL of Na 2 PdCl 4 6mM solution, add water to make the system 10mL, add 0.125mL sodium formate 2M after adsorption at 40°C for 30 minutes. The final palladium ion concentration was 2 mM and the sodium formate concentration was 25 mM. The Erlenmeyer flask was placed at 30° C. for 36 h (natural pH 3.4) to obtain a bionano palladium solution. The solution turned from yellow to black and particles were produced. The formation of biological nano-palladium solution was verified by ultraviolet-visible spectroscopy and transmission electron microscopy. The characterization results are as follows Figure 6-8 shown.
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