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Polysaccharides of Acanthopanax polysaccharides, extraction and purification methods, composition analysis methods and uses

A technology of Radix Rhizoma and extraction method, applied in the field of natural plant active ingredients, extraction and purification method, Radix Rhizoma Polysaccharide, extraction and purification, composition analysis field, and can solve the problem of inability to obtain Radix Rhizoma Polysaccharide, extraction method Single, low extraction yield, etc.

Active Publication Date: 2016-09-28
WEIFANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] As mentioned above, the prior art has disclosed a variety of extraction methods, composition analysis, and application research of Acanthopanax polysaccharides, but these methods have certain defects, such as low extraction yield, single extraction method, etc., and The molecular weight of the polysaccharides obtained from Acanthus fragrans is within a certain range, and it is impossible to obtain polysaccharides from Acanthopanax polysaccharides in other molecular weight ranges, and the composition analysis method still needs to be expanded and improved, etc.

Method used

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  • Polysaccharides of Acanthopanax polysaccharides, extraction and purification methods, composition analysis methods and uses
  • Polysaccharides of Acanthopanax polysaccharides, extraction and purification methods, composition analysis methods and uses
  • Polysaccharides of Acanthopanax polysaccharides, extraction and purification methods, composition analysis methods and uses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0101] A. Raw material pretreatment

[0102] Grinding the stem bark of the dried five-five-scented root, crossing a 100-mesh sieve to obtain the five-scented five-stem powder; soaking the powder with acetone (the mass-to-volume ratio of the two is 1:6g / ml) to make it fully swell, and then Filter to obtain powder after swelling; join the powder after the swelling in the Soxhlet extractor, use the mixed solvent of ethyl acetate and chloroform (the volume ratio of ethyl acetate and chloroform is 1:2.5, the powder and mixed solvent after swelling The mass volume ratio is 1:6.5g / ml) reflux extraction for 90 minutes, filtered while hot, to obtain the slag; fully dry the slag completely, to obtain the dry residue.

[0103] B. Step-by-step enzymatic hydrolysis

[0104] B1: At room temperature, add the dry residue of step A to deionized water (the mass ratio of dry residue to deionized water is 1:7), and then add the mixed enzyme of cellulase and xylanase (cellulase and wood The mass...

Embodiment 2-12

[0114] Embodiment 2-12: the investigation of different enzymolysis temperature

[0115] Except that only the temperature T1 in the step B1 is different from the temperature T2 in the step B2 (others are the same), the same method as that of Example 1 was used to obtain Example 2-12, based on 100g of dry Radix Acanthopanax stem bark , the amount of the obtained crude polysaccharide and the polysaccharide content are shown in the following table 1, for the convenience of comparison, the results of Example 1 are listed together.

[0116] Table 1: The influence of two enzymatic hydrolysis temperatures

[0117]

[0118]

[0119] It can be seen from the above table 1 that the temperature in the two enzymatic hydrolysis has a significant impact on the final extraction results, only when T1 is 35-45°C and T2 is 60-75°C, and the difference between T2 and T1 is 21-29 It has a better effect when T2-T1=25°C, and has the best extraction effect when T2-T1=25°C.

Embodiment 13-16

[0120] Embodiment 13-16: the investigation of enzymolysis step

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Abstract

The invention relates to an extraction method, a purification method and a composition analysis method of acanthopanox giraldilharms polysaccharide as well as purified acanthopanox giraldilharms polysaccharide and a use thereof. The extraction method comprises the following steps: A, preprocessing raw materials; B, carrying out enzymolysis in different steps; C, carrying out liquid-liquid extraction; and D, carrying out alcohol precipitation, thereby obtaining the acanthopanox giraldilharms polysaccharide. According to the method, the acanthopanox giraldilharms polysaccharide with high yield and high purity can be obtained by virtue of a combination / cooperative effect of technical characteristics of unique raw material preprocessing and step-by-step enzymolysis; on the basis of the obtained crude polysaccharide, the purified polysaccharide with high purity is obtained by virtue of ion column chromatography and gel column chromatography, components of the polysaccharide are analyzed by virtue of unique composition analysis method, the components of the polysaccharide are clarified, and the purified polysaccharide can be used for adjusting a T cell subset and / or improving a IgM expression level and can be applied to the field of medicine.

Description

technical field [0001] The present invention relates to a natural plant active ingredient, an extraction and purification method, a composition analysis method and its use, more particularly to a five-five-scented polysaccharide, an extraction and purification method, a composition analysis method and its use, belonging to plant active ingredients and Its extraction, analysis and application areas. Background technique [0002] The traditional Chinese medicine Acanthopanax giraldii Hams is the stem bark of Acanthopanax giraldii Hams in the Araliaceae family. It is a famous traditional medicinal material in my country and has been widely used in the field of traditional Chinese medicine. [0003] Cortex Acanthopanax is warm in nature and pungent in taste, enters the liver and kidney meridians, and has a variety of medicinal functions, such as dispelling rheumatism, clearing joints, and strengthening bones and muscles. Weak feet and knees, skin rheumatism, impotence and sac d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00G01N30/02G01N30/06A61K31/715A61P37/02A61P43/00
Inventor 陈永梁淑娟
Owner WEIFANG MEDICAL UNIV