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Beauveria bassiana with high pathogenicity to anoplophora chinensis larva and applications thereof

A technology of Beauveria bassiana and Beauveria beauveria, which is applied in the application, pesticides, fungi and other directions, can solve the problems of serious harm, withering and loss of forestry production, and achieve strong secondary infection. ability, reducing the number of populations, and solving the effect of environmental pollution

Inactive Publication Date: 2015-05-20
FUJIAN ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Anoplophora chinensis Forster belongs to the family Cerambycidae in the order Coleoptera. It has a wide range of hosts, including 26 families, 40 genera and more than 100 species of plants. It can not only harm broad-leaved trees, but also Cryptomeria japonica Coniferous trees such as conifers eat the trunk or taproot near the ground with larvae, destroying the transportation of nutrients and water in the tree body, so that the tree vigor is weak, and the whole plant dies in severe cases. It is an important tree borer pest; As for the greening tree species, the star beetle is very harmful and has brought huge losses to forestry production
At the same time, the star beetle is also the main borer pest of Casuarina spp., the main tree species in the southeast coastal shelterbelts. In the forests where the occurrence is serious, the rate of insects reaches more than 50%, which seriously affects the protective benefits of coastal shelterbelts.
[0003] Star beetle larvae are bored into the xylem of the trunk, living in seclusion and difficult to control
It is difficult for traditional chemical agents to reach the harmful parts, resulting in unsatisfactory control effects, and the impact on the environment caused by the extensive use of chemical pesticides has attracted increasing attention

Method used

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  • Beauveria bassiana with high pathogenicity to anoplophora chinensis larva and applications thereof
  • Beauveria bassiana with high pathogenicity to anoplophora chinensis larva and applications thereof
  • Beauveria bassiana with high pathogenicity to anoplophora chinensis larva and applications thereof

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Experimental program
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Effect test

Embodiment 1

[0024] Identification of Beauveria bassiana FJBb201023

[0025] Morphological identification:

[0026] Cultivate the isolated and purified Beauveria bassiana strain on a flat PPDA medium with a diameter of 90mm at 25±1°C. After 4-5 days, pick a white hyphae preparation and observe the hyphae and distribution of the strain under a microscope. Morphology of sporozoites; 10-12 days later, pick mature conidia and make slices to observe the shape and size of conidia. Observe and record the morphological characteristics of the colony for about 15 days. The colony of this strain is white to milky white, and it is fluffy or cottony at first, and later forms a milk powder-like spore layer. The spore layer is thick and uniform, and the center is raised. Branched hyphae, regular, colorless and smooth, sometimes growing in clusters; sporogenous cells (bottle stems) are densely clustered on short and enlarged stalk cells, bottle-shaped, the upper part of the sporogenous cells is zigzag, ...

Embodiment 2

[0036] Biological Characteristics of Beauveria bassiana FJBb201023

[0037] The Beauveria bassiana strain FJBb201023 was inoculated in the center of the PPDA plate medium by the spot method, cultured in an incubator at 25±1°C, and the diameter of the colony was measured once a day by the cross method, and 3 colonies were set for each treatment Repeat for a total of 15 days of observation. At the same time, record the start time of sporulation. On the 15th day (sporulation is basically over), use a puncher with a diameter of 1.5 cm to punch a small plate from the center of the colony to the edge 1 / 2, take out the plate and place it in a 0.3 ‰ Tween-80 sterile water in the Erlenmeyer flask, shake to fully disperse the spores and properly dilute, measure the concentration of spores with a hemocytometer and convert it into the amount of sporulation per unit area. Three wells were drilled on each plate to measure the spore content, and the average value was taken as the number of ...

Embodiment 3

[0042] Determination of Pathogenicity of Beauveria bassiana FJBb201023 to Beauveria larvae

[0043] A certain amount of Beauveria bassiana (Beauveria bassiana) FJBb201023 spores were scraped from the PPDA plate and placed in sterilized 0.3‰ Tween-80 solution to prepare 10 7 Spores / mL of spore suspension (the suspension is Beauveria bassiana FJBb201023 inoculum); immerse healthy 3-instar longhorn beetle larvae in the spore suspension for 10 seconds, then put them back into the empty insect culture tube, and inoculate 30 long beetle larvae, repeated 3 times; treated with 0.3‰ Tween-80 sterile aqueous solution as a control. After the treatment, place the insect culture box in a dark 25±1°C constant temperature incubator, observe and record the death of the beetle larvae every day, and observe the growth of mycelia and sporulation of the dead insects in moisturizing culture (visible to the naked eye grows on the dead insects). hyphae and spores), and pick the slices under a micro...

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Abstract

The invention discloses beauveria bassiana with high pathogenicity to anoplophora chinensis larva and applications thereof. The beauveria bassiana strain provided by the invention is named as Beauveria bassiana FJBb201023 and is preserved in China General Microbiological Culture Collection Center (CGMCC) on October 27, 2014, and the preservation number is CGMCC No.9851. The strain is rapid in growth speed and strong in spore production capability, and has high pathogenicity to anoplophora chinensis larva, and after being inoculated with spore suspension (107 spores / mL), the lethal time of 50% (LT50) of the strain to anoplophora chinensis larva is 5.38 days. A fungal preparation produced by using the strain has a good prophylaxis and treatment effect on anoplophora macularia, and can effectively reduce the woodland population density of anoplophora macularia, therefore, the strain is a biocontrol strain having a potential value in the prevention and control of anoplophora macularia, and has a positive significance to the reduction of damages caused by anoplophora macularia.

Description

technical field [0001] The invention belongs to the field of pest biological control, and relates to a strain of Beauveria bassiana and its application, in particular to a strain of Beauveria bassiana with high pathogenicity to beetle beetle larvae and its application. Background technique [0002] Anoplophora chinensis Forster belongs to the family Cerambycidae in the order Coleoptera. It has a wide range of hosts, including 26 families, 40 genera and more than 100 species of plants. It can not only harm broad-leaved trees, but also Cryptomeria japonica Coniferous trees such as conifers eat the trunk or taproot near the ground with larvae, destroying the transportation of nutrients and water in the tree body, so that the tree vigor is weak, and the whole plant dies in severe cases. It is an important tree borer pest; As for the greening tree species, the star beetle is very harmful, which has brought huge losses to forestry production. At the same time, the star beetle is ...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P7/04C12R1/645
CPCA01N63/30C12N1/14C12N1/145C12R2001/645
Inventor 蔡守平何学友黄金水曾丽琼
Owner FUJIAN ACAD OF FORESTRY
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