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A kind of biological compound preparation containing m-methoxybenzoic acid and amino oligosaccharin and application thereof

A technology of methoxybenzoic acid and amino oligosaccharin, which is applied in the field of biological compound preparations, to achieve the effects of reduced use cost, good control effect, and improved activity

Inactive Publication Date: 2017-06-06
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These two biopesticides have good control effects on plant bacterial diseases, but in terms of production practice, it is still expected to have higher control effects and lower cost and less dosage.

Method used

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  • A kind of biological compound preparation containing m-methoxybenzoic acid and amino oligosaccharin and application thereof
  • A kind of biological compound preparation containing m-methoxybenzoic acid and amino oligosaccharin and application thereof
  • A kind of biological compound preparation containing m-methoxybenzoic acid and amino oligosaccharin and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1: The present invention is to the inhibitory effect test of bacterial wilt pathogen Laueria.

[0015] Step 1: Preparation of liquid medicine

[0016] Preparation of mother solution: m-methoxybenzoic acid and 3% oligosaccharin parent drug were diluted with 1% DMSO and 0.1% Tween-80 aqueous solution respectively, and were prepared into a single-dose mother solution of 1000mg / L respectively, and then 7 groups of compound mother liquors were compounded at volume ratios of 5:1, 3:1, 2:1, 1:1, 1:2, 1:3, and 1:5, respectively, and set aside.

[0017] Concentration setting: According to the activity of the drug, the single agent mother solution of m-methoxybenzoic acid and amino oligosaccharin, and the seven groups of compound agent mother solutions were respectively configured to 500mg / L, 250mg / L, 125mg / L, 62.5mg / L L, 31.25mg / L 5 serial concentrations of liquid medicine, each concentration of liquid medicine set 3 repeated tests.

[0018] Step 2: The test target ...

Embodiment 2

[0032] Embodiment 2: The present invention is tested on the inhibitory effect of melon fruit spot disease pathogen Acidovorax citrulli.

[0033] The test procedure is basically the same as that of Example 1, except that the test target is the melon fruit blotch pathogen: Acidophilus genus watermelon species.

[0034] In step 4, according to the measurement results, the bactericidal effect and toxicity regression equation of the test agent were calculated, and the results are shown in Table 2.

[0035] Table 2 biological compound preparation of the present invention is to the indoor toxicity determination result of melon fruit spot disease pathogen Acidophilus genus watermelon species

[0036]

[0037] As can be seen from Table 2, the volume ratio of m-methoxybenzoic acid and amino oligosaccharin is between 5: 1 and 1: 5. The co-toxicity coefficient is 56.86 to 178.16, wherein the ratio is 2: 1, 1: 1, The synergistic effect on acidophilus watermelon species was obvious at 1...

Embodiment 3

[0038]Example 3: Test of the inhibitory effect of the present invention on bacterial angular leaf spot pathogen Pseudomonas syringa epv.tomato.

[0039] The test procedure is basically the same as that of Example 1, except that the test target is the pathogen of bacterial angular leaf spot: Pseudomonas syringae pv. tomato.

[0040] In step 4, according to the measurement results, the bactericidal effect and toxicity regression equation of the test agent were calculated, and the results are shown in Table 3.

[0041] Table 3 biological compound preparation of the present invention is to the indoor toxicity test result of bacterial angular leaf spot pathogen Pseudomonas syringae pathogenic variety of tomato

[0042]

[0043]

[0044] It can be seen from Table 3 that the volume ratio of m-methoxybenzoic acid and amino oligosaccharin is between 5: 1 and 1: 5, and the co-toxicity coefficient is 84.74 to 185.83, when the ratio is 1: 1 and 3: 1 The synergistic effect on the pa...

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Abstract

The invention relates to a biological compound preparation containing m-methoxybenzoic acid and amino-oligosaccharin and application of the biological compound preparation and aims to provide a preparation capable of exerting the efficacies of m-methoxybenzoic acid and amino-oligosaccharin to the maximum extent and generating an obvious synergic effect on preventing and treating plant bacterial diseases, so that the use amount is reduced and the using cost is lowered. The preparation is compounded by a 1000mg / L m-methoxybenzoic acid mother liquor and a 1000mg / L amino-oligosaccharin mother liquor in a volume ratio of (1 to 5) to (5 to 1), preferably 1 to 1.

Description

technical field [0001] The invention relates to a biological compound preparation and its application, in particular to a biological compound preparation containing m-methoxybenzoic acid and amino oligosaccharin for preventing and controlling plant pathogenic bacteria and its application. Background technique [0002] Mikania micrantha belongs to the genus Eupatorium of the Asteraceae family. It is a perennial creeping weed with extremely fast growth. It has spread all over my country's Yunnan, Guangdong, Hainan, Guangxi, Hong Kong, Taiwan and other provinces, seriously destroying the biodiversity and ecology of the invaded areas. environment and cause huge economic losses. However, in its place of origin, Mikania micrantha is used as an antibiotic and anti-inflammatory agent for treating various skin diseases such as wound infection, psoriasis, athlete's foot and snake venom. The lactone active substances extracted from Mikania micrantha have been reported in many literatur...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/16A01P1/00A01N37/40
Inventor 李君曹坳程毛连纲李园王秋霞颜冬冬郭美霞欧阳灿彬
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI