A kind of preparation method of yoghurt
A technology of yogurt and fermentation method, which is applied in the direction of milk preparations, dairy products, applications, etc. It can solve the problems of unremarkable typical flavor of yogurt, unbalanced ratio of bacilli and cocci, and poor coagulation state of yogurt, so as to achieve suitable and refreshing sour taste and acid production High volume, the effect of solving poor solidification state
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Embodiment 1
[0036] The concrete breeding process of described plantarum lactobacillus is as follows:
[0037] 1. Diethyl Sulfate (DES) Mutation Breeding
[0038] (1) Take a ring of Lactobacillus plantarum on the inclined surface of the test tube on the ultra-clean bench, insert it into a 250mL Erlenmeyer flask containing 50mL of liquid MRS xylan medium, and culture it at 200rpm at 40°C for about 12h, so that the bacteria are in the right condition. Early growth period.
[0039] (2) Take 5 mL of the bacterial liquid, centrifuge at 5000 rpm for 10 minutes to collect the bacterial cells, and wash twice with normal saline.
[0040] (3) Dilute to 10 with pH7.0 phosphate buffer 7 individual / mL bacterial suspension.
[0041] (4) Take 32mL of pH7.0 potassium phosphate buffer, 8mL of bacterial suspension, and 0.4mL of DES in a 150mL Erlenmeyer flask pre-placed in the rotor and mix thoroughly so that the final concentration of DES is 1% (v / v).
[0042] (5) React in a shaker at 30°C at 150rpm fo...
Embodiment 2
[0058] A method for preparing yogurt, comprising the steps of:
[0059] (1) Acceptance of raw milk: choose milky white or slightly yellow, acidity 15°T, temperature 4°C, and the number of miscellaneous bacteria is not higher than 500,000 CFUmL -1 , with a total dry matter content of 13.5%, of which non-fat dry matter is 8.6%, fresh milk without residual antibiotics, bactericides, preservatives, and adulteration, for standby;
[0060] (2) Sterilization storage: use a sterilizer, high temperature pasteurization, temperature 93°C, time 5min.
[0061] (3) Standardization and ingredients: standardize the fat and protein of the raw milk before batching, pour the raw milk into the batching tank and heat it in a cycle until the temperature reaches 45°C, add 7% milk powder and 4.5% sweetener , 0.3% essence, at the same time turn on the stirring and high-speed emulsification pump to make the material, the cycle time is 30 minutes;
[0062] The sweetener is xylitol;
[0063] (4) Degas...
Embodiment 3
[0073] A kind of preparation method of yoghurt, concrete steps are as follows:
[0074] (1) Acceptance of raw milk: choose milky white or slightly yellow, acidity at 16°T, temperature at 7°C, and the number of bacteria not higher than 500,000 CFU mL -1 , the total dry matter content is 12.5%, of which the non-fat dry matter is 8.6%, fresh milk without residual antibiotics, bactericides, preservatives, and adulteration, for standby;
[0075] (2) Sterilization storage: use a sterilizer, high temperature pasteurization, temperature 95°C, time 5min.
[0076] (3) Standardization and batching: after standardizing the fat and protein of raw milk, batching is carried out, and the raw milk is put into the batching tank for circulation heating. Sweetener, 0.1% essence, simultaneously turn on the stirring and high-speed emulsification pump to chemicalize the material, and the cycle time is 30 minutes;
[0077] The sweetener is acesulfame K and aspartame, and the mixing ratio of the ace...
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