Multiple PCR detection kit for three bacterial seed-borne diseases and special primer for kit as well as multiple PCR detection method
A bacterial leaf spot and bacterial technology, which is applied in biochemical equipment and methods, microorganism-based methods, and microbial determination/inspection, etc. 3. Detection has limitations and other problems, to achieve the effect of saving detection cost and time, saving detection time and reducing detection cost
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[0080] Embodiment 1 The design of the special primer Xj for the rapid detection of tomato canker of the present invention and the detection of tomato canker by conventional molecular biological methods using the pair of special primers Xj.
[0081] (1) Design and synthesis of special primer Xj for detection of tomato canker
[0082] The special primer Xj for the detection of tomato canker is based on the 16SrDNA gene fragment in the chromosomal genome of tomato canker. DNAMAN6.0 is used for homology analysis to determine the highly conserved region, and then Primer 6.0 primer design software is used to design specific primers, The number is dedicated primer Xj, which is composed of the upstream primer of the dedicated primer Xj and the downstream primer of the dedicated primer Xj, the base sequence of the upstream primer of the dedicated primer Xj is shown in SEQ ID NO: 1, and the downstream primer of the dedicated primer Xj The base sequence of the primer is shown in SEQ ID N...
Example Embodiment
[0100] Example 2 Design of the special primer Dc for rapid detection of tomato bacterial leaf spot according to the present invention, and the detection of tomato bacterial leaf spot by conventional molecular biology method using the pair of special primers Dc.
[0101] (1) Design and synthesis of special primer Dc for detection of tomato bacterial leaf spot
[0102] Special primer Dc for detection of tomato bacterial leaf spot The special primer Dc is based on the 23S rDNA gene fragment in the chromosome genome of tomato bacterial leaf spot. DNAMAN6.0 is used for homology analysis to determine the highly conserved region, and then Primer 6.0 primers are used. Design software, design specific primers and number them as special primer Dc, which is composed of special primer Dc upstream primer and special primer Dc downstream primer, the base sequence of the special primer Dc upstream primer is as SEQ ID NO: 3 As shown, the base sequence of the downstream primer of the special pri...
Example Embodiment
[0106] Embodiment 3 is the design of the special primer At for the rapid and accurate detection of cucurbit bacterial fruit spot disease of the present invention and the detection of cucurbit bacterial fruit leaf spot by conventional molecular biology method with this special primer At.
[0107] (1) Special primer At and its design and synthesis
[0108] The special primer At for the detection of cucurbit bacterial fruit spot is based on the hrpB2 gene fragment in the chromosomal genome of cucurbit bacterial fruit spot, and DNAMAN6.0 is used to conduct homology analysis to determine the highly conserved region, and then use Primer 6.0 primer design software , design a specific primer, and number it as a special primer At, which is composed of a special primer At upstream primer and a special primer At downstream primer, the base sequence of the special primer At upstream primer is shown in SEQ ID NO: 5 , the base sequence of the downstream primer of the special primer At is sh...
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