Small peptide with antioxidant activity, preparation method and application thereof
An antioxidant activity and linear technology, applied in the biological field, can solve the problems of artificial antioxidant application limitation, influence on enzyme activity, damage, etc., and achieve the effects of low price, improved stability and simple method.
Active Publication Date: 2018-08-10
NANJING UNIV OF FINANCE & ECONOMICS
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Problems solved by technology
However, long-term use will damage the liver, spleen, and lungs of the human body, have cumulative carcinogenic effects, and affect the activity of enzymes related to breathing. Therefore, the application of artificial antioxidants is limited.
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Embodiment 1
[0028] Example 1 Preparation of small peptides with antioxidant activity
[0029] (1) Extraction of wheat germ albumin: Accurately weigh 100 g of defatted wheat germ powder, add 1000 ml of deionized water, mix well, stir and extract in a water bath at 40 ° C for 1 h; centrifuge at 8000 rpm for 30 min, take the supernatant and slowly add ammonium sulfate to The saturation is 75%, stirred at 4° C. for 2 h; centrifuged at 12,000 rpm for 20 min, the supernatant is discarded, and the precipitate is collected, which is wheat germ albumin. For subsequent purification, the wheat germ albumin was dialyzed to desalt and freeze-dried to obtain the wheat germ albumin powder.
[0030] (2) Trypsin hydrolysis: Dissolve wheat germ albumin powder with pH 7.6, 0.1M phosphate buffer solution, add 7000U trypsin per gram of protein (purchased from Shanghai Sangon Bioengineering Co., Ltd., 2500000U / g) , Enzymolysis at 37°C for 6h to obtain albumin hydrolyzate Ⅰ. Phosphate buffer solution with a p...
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Abstract
The invention discloses a small peptide with antioxidant activity as well as a preparation method and application thereof and belongs to the biological technical field. The amino acid sequence of the small peptide is AREGETVVPG. The preparation method of the small peptide comprises the following steps: performing trypsin enzymolysis on a wheat germ albumin to obtain an albumin hydrolysate I; getting a compound in the albumin hydrolysate I with the molecular weight of less than 10 KDa, performing gel filtration chromatography, and taking the component with antioxidant activity as an albumin hydrolysate II; performing alkaline protease enzymolysis on the albumin hydrolysate II to obtain an albumin hydrolysate III; and sequentially performing gel filtration chromatography and reversed-phase high-performance liquid chromatography on the albumin hydrolysate III to obtain the small peptide. The small peptide provided by the invention has strong antioxidant activity, is derived from the wheat germ albumin, has the characteristics of safe, effective, simple preparation method and good repeatability, and can be applied to increase the food stability and the storage period or prepare an antioxidant activity healthcare food.
Description
technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a small peptide with antioxidant activity, a preparation method and an application thereof. Background technique [0002] After the theory of free radicals was proposed, many research results have shown that free radicals are related to the occurrence of various human diseases and aging, such as atherosclerosis, thrombosis, myocardial ischemia-reperfusion injury, diabetes, cancer and neurodegenerative diseases. Reactive oxygen species (ROS) are free radicals widely present in the human body, produced by mitochondria, including superoxide anion radicals, hydroxyl radicals, etc., which can damage mitochondrial DNA, enzymes and membrane lipids, thereby causing various diseases happened. Antioxidants have the ability to capture and neutralize free radicals. When the free radicals in the body are too much and exceed the scope of scavenging ability, supplementing antioxidants ...
Claims
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IPC IPC(8): C07K7/06C12P21/06C07K1/16C07K1/20A23L33/18A23L3/3544
Inventor 沈新春陈思远刘永祥曹小舟苏安详俞凌
Owner NANJING UNIV OF FINANCE & ECONOMICS