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A kind of biological macromolecular microcapsule product and preparation method thereof

A technology of biological macromolecules and microcapsules, which is applied in the direction of microcapsules, capsule delivery, medical preparations of non-active ingredients, etc., and can solve the problems of oral bioavailability lower than 1%, low specific toxicity of polypeptide protein drugs, etc. , to achieve the effect of reducing enzymatic and acid degradation, reducing the release rate and increasing the pore size

Inactive Publication Date: 2017-10-17
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Polypeptide protein drugs have the characteristics of high activity, strong specificity, and low toxicity, and can effectively treat difficult diseases such as cancer, autoimmune diseases, and hypertension, but their oral bioavailability is usually lower than 1%. Injection-based

Method used

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  • A kind of biological macromolecular microcapsule product and preparation method thereof
  • A kind of biological macromolecular microcapsule product and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] As mentioned before, the yeast cells are cultured in a shake flask, and then the yeast is collected and washed.

[0058] Tween-80 of 0.1 to 5% by weight is added, shake treatment is performed at a temperature of 40 to 60°C for 24 to 48 hours, and then the temperature is raised to 85°C for enzymatic inactivation treatment for 5 to 20 minutes. After centrifugation, washing, freeze drying.

[0059] 1 g of yeast cells and 1-20 mL of 1% BSA aqueous solution were subjected to high frequency contact at 20-40°C for 24 to 48 hours, then taken out, centrifuged, washed with water to remove unembedded BSA on the surface, and freeze-dried.

[0060] Finally, the content of BSA in the microcapsules was determined by high performance liquid chromatography. The embedding degree of BSA in the obtained microcapsules is 2.54% to 3.02%.

Embodiment 2

[0062] The steps of culturing, processing yeast and embedding are the same as in Example 1, except that the added chemical reagent is TritonX-100 with a weight of 0.1 to 5%. The embedding degree of BSA in the obtained microcapsules is 2.47% to 2.98%.

Embodiment 3

[0064] The steps of culturing, treating yeast and embedding are the same as in Example 1, except that the chemical reagent added is 0.1-5% by weight cetyltrimethylammonium bromide. The embedding degree of BSA in the obtained microcapsules is 1.32% to 2.33%.

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Abstract

The invention provides a method for preparing biomacromolecule microcapsules, which relates to methods for culturing and treating yeast cells, preparing microcapsules and sealing holes. Yeast cells cultured in shake flasks under appropriate conditions are treated with chemical reagents such as Tween-80 or TritonX-100, and then the temperature is raised to inactivate the yeast cells. Centrifuged, washed, and further treated with sodium hydroxide solution. After centrifugation, washing, and freeze-drying, the yeast cell microcapsule wall material is obtained. After the yeast cell wall material is in high-frequency contact with the biomacromolecule solution to complete the encapsulation, the pores are sealed with a cationic polymer. After centrifugation, unencapsulated biomacromolecules and excess cationic polymers on the surface are washed away, freeze-dried and ground to obtain biomacromolecular yeast cell microcapsule products with uniform size, non-cohesive, and slow-release properties.

Description

Technical field [0001] The present invention provides a method for preparing biomacromolecule microcapsules, which relates to methods for culturing, processing, microcapsule preparation and sealing of yeast cells, and further relates to biomicrocapsule wall materials and biomacromolecules prepared by the method Microcapsule products. Background technique [0002] As a new technology that can improve the performance of functional substances, microcapsules have broad application prospects. Yeast cells are uniform in size, easy to cultivate, and inexpensive. The double-layered cyst structure composed of cell walls and cell membranes is a good material for embedding materials. French patent FR2179528 reported that industrial yeast was treated with a plasma wall separating agent and embedded with neodymium chloride, magnesium chloride and onion extract; US patent USA 4001480 introduced a kind of fat cultured on a medium with low nitrogen and high carbon sources. Method for embedding...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/50A61K47/46A61K38/00
Inventor 石国荣
Owner HUNAN AGRICULTURAL UNIV