A kind of preparation method of microbial spore dry suspension agent
A dry suspending agent and microorganism technology, applied in the preservation of microorganisms and other directions, can solve the problems of the death of microbial spores, the reduction of the overall activity of the product, the influence of mixing uniformity, etc., and achieve the effect of saving drying time, saving preparation time and improving production efficiency.
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Embodiment 1
[0031] This embodiment takes the preparation of the dry suspension of Bacillus licheniformis (CICC10266) as an example to illustrate the preparation method of the dry suspension of microbial spores.
[0032] The preparation steps are:
[0033] (1) In the disc centrifuge, the fermentation liquid of Bacillus licheniformis (CICC10266) is adjusted to be 500L / h by the flow meter of the feed and discharge flow, and the clear liquid is discharged by the pressure regulating valve of the clear liquid outlet of the centrifuge. Adjust the outlet pressure to 0.4MPa, set the slag discharge cycle to 5min, and obtain concentrated microbial spore liquid A at the slag discharge port of the centrifuge after the fermentation broth is processed;
[0034] (2) Preparation of the carrier: by weight, take 87 parts of diatomite, 7 parts of polyvinyl alcohol, 4 parts of sodium lauryl sulfate and 0.1 part of potassium dihydrogen phosphate and mix them, and the mixture is crushed by airflow to obtain the...
Embodiment 2
[0038] This embodiment takes the preparation of the dry suspension of Bacillus licheniformis (CICC10266) as an example to illustrate the preparation method of the dry suspension of microbial spores.
[0039] The preparation steps are:
[0040] (1) In the disc centrifuge, the fermentation liquid of Bacillus licheniformis (CICC10266) is adjusted to be 650L / h by the flow meter of the feed and discharge flow, and the clear liquid is discharged by the pressure regulating valve of the clear liquid outlet of the centrifuge. Adjust the outlet pressure to 0.41MPa, set the slag discharge cycle to 4min, and obtain concentrated microbial spore liquid A at the slag discharge port of the centrifuge after the fermentation broth is processed;
[0041] (2) Preparation of carrier: by weight, take 80 parts of diatomaceous earth, 5 parts of dispersant MorwetD-425, 2 parts of sodium lauryl sulfate and 0.05 part of sorbitol and mix, and the mixture is jet-pulverized to obtain the carrier ;
[004...
Embodiment 3
[0045] This embodiment takes the preparation of the dry suspension of Bacillus licheniformis (CICC10266) as an example to illustrate the preparation method of the dry suspension of microbial spores.
[0046] The preparation steps are:
[0047] (1) In the disc centrifuge, the fermentation liquid of Bacillus licheniformis (CICC10266) is adjusted to be 1000L / h by the flow meter of the feed and discharge flow, and the clear liquid is discharged by the pressure regulating valve of the clear liquid outlet of the centrifuge. Adjust the outlet pressure to 0.42MPa, set the slag discharge period to 3min, and obtain concentrated microbial spore liquid A at the slag discharge port of the centrifuge after the fermentation broth is processed;
[0048] (2) Preparation of carrier: by weight, take 90 parts of diatomite, 10 parts of dispersant TERSPENSE2700, 6 parts of pull-off powder and 0.2 part of trehalose and mix, and the mixture is jet-pulverized to obtain the carrier;
[0049] (3) Add t...
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