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A kind of preparation method of microbial spore dry suspension agent

A dry suspending agent and microorganism technology, applied in the preservation of microorganisms and other directions, can solve the problems of the death of microbial spores, the reduction of the overall activity of the product, the influence of mixing uniformity, etc., and achieve the effect of saving drying time, saving preparation time and improving production efficiency.

Active Publication Date: 2018-11-27
NINGBO JIANGDONG HUIZE BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] For example, the Chinese invention patent application with application publication number CN102027999A "A Bacillus thuringiensis wettable powder containing pullulan polysaccharide and its preparation method" (application number: 201010549039.0) discloses a preparation method of a spore wettable powder, Since the drying temperature is 50-70°C, the temperature is high, which can easily reduce the activity of living bacteria in the product and affect the use effect.
[0005] Moreover, due to the need for secondary treatment when preparing the dry suspension of microbial spores in the prior art, not only the preparation method is complicated and the yield is low, but also the product after the primary processing and the additives added in the secondary processing The uniformity of mixing, carriers, etc. is affected, which in turn leads to a decrease in the overall activity of the product
At the same time, in the existing processing technology, microbial spores inevitably need to undergo high-temperature dehydration, strong airflow impact and other processes. During drying, the inlet air temperature is as high as 175°C, and the outlet air temperature reaches 60°C. A large number of microbial spores die, and the death rate of effective living bacteria reaches 20-30%, resulting in increased product costs and reduced product yields
However, if the drying temperature is lowered, the moisture content in the product will exceed the standard.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] This embodiment takes the preparation of the dry suspension of Bacillus licheniformis (CICC10266) as an example to illustrate the preparation method of the dry suspension of microbial spores.

[0032] The preparation steps are:

[0033] (1) In the disc centrifuge, the fermentation liquid of Bacillus licheniformis (CICC10266) is adjusted to be 500L / h by the flow meter of the feed and discharge flow, and the clear liquid is discharged by the pressure regulating valve of the clear liquid outlet of the centrifuge. Adjust the outlet pressure to 0.4MPa, set the slag discharge cycle to 5min, and obtain concentrated microbial spore liquid A at the slag discharge port of the centrifuge after the fermentation broth is processed;

[0034] (2) Preparation of the carrier: by weight, take 87 parts of diatomite, 7 parts of polyvinyl alcohol, 4 parts of sodium lauryl sulfate and 0.1 part of potassium dihydrogen phosphate and mix them, and the mixture is crushed by airflow to obtain the...

Embodiment 2

[0038] This embodiment takes the preparation of the dry suspension of Bacillus licheniformis (CICC10266) as an example to illustrate the preparation method of the dry suspension of microbial spores.

[0039] The preparation steps are:

[0040] (1) In the disc centrifuge, the fermentation liquid of Bacillus licheniformis (CICC10266) is adjusted to be 650L / h by the flow meter of the feed and discharge flow, and the clear liquid is discharged by the pressure regulating valve of the clear liquid outlet of the centrifuge. Adjust the outlet pressure to 0.41MPa, set the slag discharge cycle to 4min, and obtain concentrated microbial spore liquid A at the slag discharge port of the centrifuge after the fermentation broth is processed;

[0041] (2) Preparation of carrier: by weight, take 80 parts of diatomaceous earth, 5 parts of dispersant MorwetD-425, 2 parts of sodium lauryl sulfate and 0.05 part of sorbitol and mix, and the mixture is jet-pulverized to obtain the carrier ;

[004...

Embodiment 3

[0045] This embodiment takes the preparation of the dry suspension of Bacillus licheniformis (CICC10266) as an example to illustrate the preparation method of the dry suspension of microbial spores.

[0046] The preparation steps are:

[0047] (1) In the disc centrifuge, the fermentation liquid of Bacillus licheniformis (CICC10266) is adjusted to be 1000L / h by the flow meter of the feed and discharge flow, and the clear liquid is discharged by the pressure regulating valve of the clear liquid outlet of the centrifuge. Adjust the outlet pressure to 0.42MPa, set the slag discharge period to 3min, and obtain concentrated microbial spore liquid A at the slag discharge port of the centrifuge after the fermentation broth is processed;

[0048] (2) Preparation of carrier: by weight, take 90 parts of diatomite, 10 parts of dispersant TERSPENSE2700, 6 parts of pull-off powder and 0.2 part of trehalose and mix, and the mixture is jet-pulverized to obtain the carrier;

[0049] (3) Add t...

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PUM

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Abstract

The invention relates to a preparation method of a microbial brood-gemma dry suspending agent. According to the method, when a carrier is mixed with a concentrated microbial brood-gemma liquid, the concentrated microbial brood-gemma liquid is directly sprayed onto the carrier at a low-temperature of 30-35DEG C, so that the concentrated microbial brood-gemma liquid can be fully and uniformly mixed with the carrier, and the water content of the mixture of the concentrated microbial brood-gemma liquid and the carrier is low. Compared with the prior art, the method has the advantages as follows: water in the mixture to be dried is reduced by about 90% so that the drying time is greatly saved; the mixture can be directly dried to meet a standard by air at 30-40DEG C during a granulation process, and since the temperature is close to a microbial fermentation temperature, the mortality of living bacteria is greatly reduced during the drying process and the product is enabled to keep high activity; primary processing and secondary processing in the prior art are combined into one, so that the method is simple and can be used for effectively improving the production efficiency.

Description

technical field [0001] The invention relates to the technical field of post-treatment methods for microbial spores, in particular to a method for preparing a dry suspension of microbial spores. Background technique [0002] The microbial spores produced by liquid fermentation are post-processed to prepare dry suspensions, which is convenient for the preservation and application of microbial spores. [0003] At present, in the post-treatment method of microbial spores produced by liquid fermentation and the processing methods of related products, the liquid fermentation microbial spores are generally concentrated and centrifuged in a centrifuge to obtain concentrated microbial spores, and then the concentrated microbial spores are passed through Primary processing and secondary processing are used to prepare dry suspensions. Among them, the primary processing refers to mixing the concentrated microbial spore liquid with the carrier, and making the original microbial spore po...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/04
CPCC12N1/04
Inventor 任锡亮韩飞潘龙琳
Owner NINGBO JIANGDONG HUIZE BIOLOGICAL TECH CO LTD
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