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Composition and application for culturing oocytes and skin adult stem cells

A technology of adult stem cells and compositions, applied in the direction of non-embryonic pluripotent stem cells, germ cells, animal cells, etc., which can solve the problems of difficult application

Active Publication Date: 2018-07-03
LANNUO BIOTECH WUXI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0015] The above applications all involve the in vitro expansion and culture of skin adult stem cells. However, skin adult stem cells are easily differentiated into fibroblasts in vitro, making the application of autologous skin adult stem cells and artificial skin constructed from autologous skin adult stem cells clinically difficult. Realize, the application of above-mentioned composition can effectively solve this problem

Method used

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  • Composition and application for culturing oocytes and skin adult stem cells
  • Composition and application for culturing oocytes and skin adult stem cells
  • Composition and application for culturing oocytes and skin adult stem cells

Examples

Experimental program
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Effect test

Embodiment 1

[0072] This example provides a composition for culturing oocytes and skin stem cells, labeled LN01, which is composed of cysteamine and LIF, and the ratio of cysteamine to LIF is 100mmol:10 3 IU.

[0073] This example provides a composition for culturing oocytes and skin stem cells, labeled LN011, which is composed of cysteamine and LIF, and the ratio of cysteamine to LIF is 1 mmol: 1000 IU.

[0074] This embodiment provides a composition for culturing oocytes and skin stem cells, labeled LN012, which is composed of cysteamine and LIF, and the ratio of cysteamine and LIF is 50.5 mmol: 505 IU.

[0075] This example provides a composition for culturing oocytes and skin stem cells, labeled LN014, which is composed of cysteamine and LIF, and the ratio of cysteamine and LIF is 100mmol: 10IU.

Embodiment 2

[0077]This example provides a composition for culturing oocytes and skin stem cells, labeled LN02, which is composed of cysteamine, LIF and Y27632, and the ratio of cysteamine, LIF and Y27632 is 100mmol: 10 3 IU: 10mmol.

[0078] This example provides a composition for culturing oocytes and skin stem cells, labeled LN021, which is composed of cysteamine, LIF and Y27632, and the ratio of cysteamine, LIF and Y27632 is 1 mmol: 10 IU: 10 mmol .

[0079] This example provides a composition for oocyte and skin stem cell culture, labeled LN022, which is composed of cysteamine, LIF and Y27632, and the ratio of cysteamine, LIF and Y27632 is 50.5mmol: 100 IU : 0.1 mmol.

[0080] This example provides a composition for oocyte and skin stem cell culture, labeled LN023, which is composed of cysteamine, LIF and Y27632, and the ratio of cysteamine, LIF and Y27632 is 100mmol: 505IU: 5mmol .

[0081] This example provides a composition for culturing oocytes and skin stem cells, labeled LN...

Embodiment 3

[0084] This example provides a culture solution for the culture of goat oocytes, IVF fertilized eggs or IVF embryos. The cystamine concentration was 50 mM, the LIF concentration was 500 IU / mL, and the Y27632 concentration was 5 mM.

[0085] This example provides a culture medium for the culture of goat oocytes, IVF fertilized eggs or IVF embryos. The cystamine concentration was 100 mM, the LIF concentration was 10 3 IU / mL, Y27632 concentration is 10 mM.

[0086] This example provides a culture solution for the culture of goat oocytes, IVF fertilized eggs or IVF embryos, labeled as LNCSG03, added with a composition composed of cysteamine, leukemia inhibitory factor and Rho kinase inhibitor, half The cystamine concentration was 200 mM and the LIF concentration was 2 × 10 3 IU / mL, Y27632 concentration is 20mM.

[0087] This example provides a culture solution for the culture of goat oocytes, IVF fertilized eggs or IVF embryos, labeled as LNCSG04, added with a composition com...

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Abstract

The invention provides a composition and application for culturing oocytes and skin adult stem cells. The composition can increase the in vitro maturation rate of oocytes, increase the division rate of eggs, and the growth rate of embryos from developing to mulberries and blastocysts. Ratio, thereby greatly promoting the development of fertilized eggs from in vitro fertilization to embryos. At the same time, the composition can effectively inhibit the differentiation of skin adult stem cells into fibroblasts, or maintain the self-renewal and regeneration of skin adult stem cells. The composition includes cysteamine, leukemia inhibitory factor and Rho kinase inhibitor Y27632.

Description

technical field [0001] The invention relates to a composition and application for culturing oocytes and skin adult stem cells. Background technique [0002] Oocytes are produced on the mother's ovary. Follicles on the ovary include oocytes and follicular cells. During the growth of the follicle, the primary oocyte (primary oocyte) stops in the prophase of the first mature division of meiosis. When puberty begins, under the action of follicle stimulating hormone (FSH), the progenitor primary oocyte The cell volume increases and forms a zona pellucida around the periphery of the oocyte. The follicle cells grow into multiple layers of cells, surround the oocyte, and form a follicle cavity in the follicle, so it is called a secondary follicle. . In the mature follicle, the primary oocyte completes the first meiosis under the action of the hormone LH and other factors, forms a secondary oocyte, or a mature oocyte, and discharges the first polar body, polar body The body is lo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/075C12N5/074A61K45/06A61P17/02A61K8/98
Inventor 杜福良杨澜安礼友薛非
Owner LANNUO BIOTECH WUXI
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