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Application of yeast containing active polysaccharides and nucleotides in dairy cow feed

A technology of active polysaccharides and dairy cow feed, applied in the field of biochemistry, can solve the problems of economic loss in aquaculture, increased elimination rate, and affect milk quality, etc., and achieve the effects of improving non-specific immunity, solving environmental pollution, and reducing mortality

Inactive Publication Date: 2015-08-26
NANJING BIOTOGETHER
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Immune function is the basis for maintaining the health of dairy cows. The occurrence of various diseases of dairy cows is related to immune function. If the body is immunosuppressed, it will lead to the occurrence of various diseases. The occurrence of these diseases will reduce the production performance and reproductive performance, affecting The quality of milk will lead to an increase in the elimination rate, which will cause huge economic losses to the breeding industry (Xue Junxin, Zhang Kechun, Factors affecting the immune function of dairy cows [J], Dairy Science and Technology, 2011.3:142-144)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Insert the Saccharomyces cerevisiae (CGMCC No.7522) strain into a 10T fermenter for cultivation, the medium is: glucose 30g / L, (NH 4 ) 2 SO 4 4g / L, H 3 PO 4 1.5ml / L, MgSO 4 1.5g / L, FeSO 4 0.8g / L, adjust the pH of the culture medium to 4.0 with ammonia water, and ferment at 50-300rpm at 25-40°C for 10-30 hours. A decanter separator is used for solid-liquid separation, and the obtained yeast mud is added with water to form a yeast liquid with a dry solid content of 15%. Raise the temperature to 90°C, keep the temperature for 30 minutes, then cool down to 60°C. Add 0.1% g / mL neutral protease (enzyme activity 30000U / ml) and 0.1% v / v nuclease P1 (enzyme activity 2200U / ml) respectively, after 5 hours the reaction ends, and it is sprayed into powder to obtain yeast containing active polysaccharides and nucleotides.

Embodiment 2

[0032] Select 20 milk cows with similar body conditions and close time before delivery to be divided into two groups, and the control group is fed with basal feed (the basal feed formula is: corn 60wt%, soybean meal 26.0wt%, puffed soybean 6.0wt%, wheat bran 5.0wt% , import fish meal 2.0wt%, soybean oil 1.0wt%, the following examples are the same), the test group adds 10wt% yeast (prepared according to the method of Example 1) containing active polysaccharides and nucleotides to replace the equivalent basal feed . Wherein the active polysaccharide content in the yeast is 3wt%, and the nucleotide content is 5wt%. The experiment started from 1 week before delivery until after delivery, and calf colostrum samples and calf blood samples were taken. The calf serum albumin content in the experimental group was 280 mg / dL, and that in the control group was 270 mg / dL. The mutation rate of rotavirus in colostrum was 37% relative to the control group.

Embodiment 3

[0034] Select 20 milk cows with close body condition and close time before delivery and divide them into two groups. The control group is fed with basal feed, and the test group adds 15 wt% yeast containing active polysaccharides and nucleotides (prepared according to the method of Example 1) to replace the same amount of basal feed. Wherein the active polysaccharide content in the yeast is 5wt%, and the nucleotide content is 10wt%. The experiment started from 2 weeks before delivery until after delivery, and calf colostrum samples and calf blood samples were taken. The calf serum albumin content was 370mg / dL, and the control group was 270mg / dL. The mutation rate of rotavirus in colostrum was 52% relative to the control group.

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Abstract

The invention discloses the application of a yeast containing active polysaccharides and nucleotides in a dairy cow feed. The yeast containing active polysaccharides and nucleotides contains such active polysaccharide components as glucan, mannan and mannan oligosaccharide, and the total content of the active polysaccharides is 1-20wt% of that of the yeast; the yeast also contains such nucleotides as adenylate, cytidine monophosphate, guanylic acid and uridylic acid, and the total content of the nucleotides is 1-20wt% of that of the yeast. According to the method, the active feed is added to the dairy cow feed, the serum albumin of a calf can be increased by 140mg / dL by use of the methods of optimizing charging and feeding conditions and the like, and the maximum mutation rate of the rotavirus in the colostrum can be 75%. The application of the yeast containing active polysaccharides and nucleotides in the dairy cow feed has the advantages that 1, the yeast feed is capable of increasing the content of the serum albumin of the calf and boosting the immunity of the calf, and 2, the yeast feed is capable of mutating a large amount of rotavirus in the colostrum and preventing the rotavirus from pathopoiesis.

Description

technical field [0001] The invention belongs to the technical field of biochemistry, in particular to a novel active polysaccharide (dextran, mannan, mannan oligosaccharide) and nucleotide (adenylic acid, cytidylic acid, guanosine) prepared by industrial waste yeast acid, uridine acid) yeast feed. Background technique [0002] Yeast cells are rich in nutrients, in addition to a variety of essential amino acids and essential fatty acids, rich in vitamins, minerals, a variety of digestive enzymes and growth stimulators. It also contains a certain amount of RNA and a large amount of active polysaccharides-β-glucan and mannan oligosaccharides. [0003] Yeast cells are rich in nutrients, but the composition of yeast cell walls is complex, and the nutrients and physiologically active substances in the cells are difficult to be effectively absorbed and utilized by animals, and most of them are excreted as waste from animals. Broken yeast is a product that breaks the cell wall of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/18A23K1/16A23K1/00
Inventor 陈晓春张磊蔡家栋汤亦文周昌祝
Owner NANJING BIOTOGETHER
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