Method for extracting salicin from white willow bark

A technology for salicin and extract, which is applied in the field of extracting salicin from white willow bark, can solve the problems of unfavorable powder control by quicklime method, high extraction efficiency, simple process and high cost performance, achieves solvent recycling, reduces environmental pollution, The effect of easy industrialization

Active Publication Date: 2015-08-26
内蒙古昶辉生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above-mentioned methods have their own advantages and disadvantages. The quicklime method is not conducive to the powder control in the production workshop bu

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1: a kind of method extracting salicin from white willow bark, comprises the steps:

[0040] (1) Take the raw materials, cut or crush them into 1-2cm pieces, and irradiate them with ultraviolet rays for 40 minutes;

[0041] (2) Add 8 times of water to the raw material after deactivating the enzyme, add 5% by weight of salt, soak at room temperature for 1.5 hours, and filter to obtain the soaked raw material;

[0042] (3) Add water 12 times the weight of the raw material to the soaked raw material, add cellulase: alkaline protease compound enzyme (1:2), the amount of the compound enzyme is 1% by weight of the raw material, at 20-40°C Enzymatic hydrolysis for 3 hours, ultrasonic treatment of the enzymatic hydrolysis solution for 15 minutes every 15 minutes, the ultrasonic frequency is 60KHz, and the ultrasonic power is 250W, to obtain the enzymatic hydrolysis solution;

[0043] (4) After the enzymolysis solution is concentrated, it passes through a polyamide c...

Embodiment 2

[0045] Embodiment 2: a kind of method extracting salicin from white willow bark, comprises the steps:

[0046] (1) Take the raw materials, cut or crush them into 1-2cm pieces, and irradiate them with ultraviolet rays for 30 minutes;

[0047](2) Add 5 times of water to the enzyme-inactivated raw material, add 3% by weight of salt, soak at room temperature for 2 hours, and filter to obtain the infiltrated raw material;

[0048] (3) Add water 1.5 times the weight of the raw material to the soaked raw material, add cellulase: alkaline protease compound enzyme (1:2), the amount of the compound enzyme is 2% by weight of the raw material, at 20-40°C Enzymolysis for 2 hours, ultrasonic treatment of the enzymatic solution for 15 minutes every 15 minutes, the ultrasonic frequency is 40KHz, the ultrasonic power is 250W, and the enzymatic solution is obtained;

[0049] (4) After the enzymolysis solution is concentrated, it passes through a polyamide chromatography column, the ratio of sa...

Embodiment 3

[0051] Embodiment 3: a kind of method extracting salicin from white willow bark, comprises the steps:

[0052] (1) Take the raw materials, cut or crush them into 1-2cm pieces, and irradiate them with ultraviolet rays for 50 minutes;

[0053] (2) Add 10 times of water to the enzyme-inactivated raw material, add 3% by weight of salt, soak at room temperature for 1.5 hours, and filter to obtain the infiltrated raw material;

[0054] (3) Add water twice the weight of the raw material to the infiltrated raw material, add cellulase: alkaline protease compound enzyme (1:2), the compound enzyme dosage is 2% by weight of the raw material, at 20-40°C Enzymolysis for 1 hour, ultrasonic treatment of the enzymatic solution for 15 minutes every 15 minutes, the ultrasonic frequency is 80KHz, and the ultrasonic power is 250W, to obtain the enzymatic solution;

[0055] (4) After the enzymolysis solution is concentrated, it passes through a polyamide chromatography column, the ratio of sample ...

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PUM

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Abstract

The invention relates to a method for extracting salicin from white willow bark. According to the method, a salicin-rich product is obtained through a process including sterilization, infiltration, enzymolysis extraction, separation and drying. The method creatively adopts the process in which enzyme deactivation and salt solution soaking are performed before enzymolysis, the kind of enzyme in enzymolysis reaction is determined by system screening, and a white willow bark extract containing more than 95 percent of salicin is obtained through twice column chromatography separation and microwave drying. Quicklime is not needed to be used in the whole method, all materials used in the method are conventional materials, the cost is low, the yield is high, a solvent can be used repeatedly, environmental pollution is reduced, and industrialization is facilitated.

Description

technical field [0001] The invention relates to a preparation method for extracting high-purity salicin from white willow bark. Background technique [0002] White willow bark is the bark of Salix alba L., a plant of Salix family Salicaceae. It has a long history of use and is mainly used for anti-inflammatory, analgesic and fever-reducing. After scientific research, salicin contained in white willow bark is the source of its anti-inflammatory and pain-relieving effects. Therefore, salicin has entered everyone's field of vision. Salicin, also known as salicin, has the molecular formula C 13 h 18 o 7 , molecular weight 286.28, white crystal, soluble in water, easily soluble in boiling water, insoluble in ethanol, insoluble in ether or chloroform, but soluble in alkali solution, pyridine or glacial acetic acid, has antipyretic, analgesic, anti Rheumatism, anti-inflammatory effect. The latest research also found that salicin, as an oxidase inhibitor, can promote skin metab...

Claims

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Application Information

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IPC IPC(8): C07H15/203C07H1/08
CPCC07H1/08C07H15/203
Inventor 高瑞白易白莲婷赵永强张成亮
Owner 内蒙古昶辉生物科技股份有限公司
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