Bacteroides fragilis selective culture medium

Abstract

The invention discloses a bacteroides fragilis culture medium, and belongs to the field of inspection microbe cultivation. The bacteroides fragilis culture medium is characterized by comprising casein tryptone, glucose, yeast powder, sodium chloride, agar, cimigenyl xyloside, pig gallbladder powder, valine, and the balance of distilled water for per 1000 ml. Compared with the prior art, the bacteroides fragilis culture medium has the advantages that the inspection result is reliable, and the separation rate of bacteroides fragilis is high.

Description

technical field [0001] The invention relates to the field of microbiology testing, in particular to a culture medium for Bacteroides fragilis. Background technique [0002] Bacteroides Fragilis (BF) is a Gram-negative short bacillus with capsules, no spores, some pili, obligate anaerobic. BF is an integral part of the normal flora of the human body, and is also an important clinically important conditional pathogen. It can cause infections in many parts of the human body. The symptoms of infection can be found in various clinical departments. The detection rate is high, accounting for 25% of clinical anaerobic isolates. Bacteroides isolates accounted for 50%, and the BF isolation rate in patients with diarrhea was 9.2%-26.8%. [0003] The clinical determination of BF is mainly based on PCR and cell culture. The sensitivity of PCR is high, and the result can be obtained within 5 hours. It has the characteristics of rapidity and sensitivity, but it has the following defects: ...

AI Technical Summary

Problems solved by technology

[0003] The clinical determination of BF is mainly based on PCR and cell culture. The sensitivity of PCR is high, and the result can be obtained within 5 hours. It has the characteristics of rapidity and sensitivity, but it has the following defects: ① Fecal samples contain PCR inhibitors, which can easily cause false negative results ; ② complex experimental conditions are required, and the cost is high

However, the BBE medium has the following defects: ① cannot inhibit the growth of Fusobacterium mutans and Fusobacterium dead; ② has no killing effect on aerobic bacteria and facultative anaerobic bacteria, so gentamicin 100mg / L should be added , but gentamicin also has a weak inhibitory effect on BF, resulting in slow growth; ③If gentamicin is not added, a strict anaerobic environment is required. Since most laboratory departments of primary hospitals lack absolute anaerobic testing equipment, Therefore, the "anaerobic tank culture method" is mostly used. The so-called anaerobic tank is a drying tank. The oxygen in the tank is exhausted through physical and chemical methods, resulting in an anaerobic environment.

Oxygen depletion in the anaerobic cylinder method takes time, affecting growth and isolation of obligate anaerobic bacteria BF