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Banana tissue culture propagation method

A banana and tissue culture technology, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of hindering the development of the seedling factory industry, slow turnover of production sites, and increase in plant area, and shorten production and cultivation. Single cycle, improved profit, improved quality effect

Inactive Publication Date: 2015-09-02
杨树东
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] 1. The single cycle of production and cultivation is long, and the general single-cycle cultivation (that is, inoculation to new medium to cultivation) time in banana tissue culture production is more than 30 days
[0004] 2. Due to the long production cycle, the turnover of the production site is slow, and a large number of cultivation racks are occupied, resulting in an increase in the area of ​​the plant
[0005] The above shortcomings are common problems in traditional tissue culture production. In the tropical and subtropical regions of southern China, banana tissue culture seedlings have become a large variety of seedlings, and there is a market demand of more than 100 million plants every year. The incremental increase (labor costs, site rent, etc.) has greatly hindered the development of the seedling industrialization industry, so a new banana tissue culture propagation technology was developed to overcome the shortcomings of the original cultivation method and ensure the growth of the banana seedling industry. Further development

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0031] A method for banana tissue culture propagation, comprising the steps:

[0032] (1) Selection of explants

[0033] Select fruit bearing for 3 years, grow vigorously, and the banana head without disease and insect damage is explant;

[0034] (2) disinfection of explants

[0035] Wash the above-mentioned banana heads with clean water first, then use 70% alcohol to spray and disinfect, and then peel off the banana heads under the condition of a sterile ultra-clean bench, and use 70% alcohol to clean each layer of bracts after peeling off. Spray and disinfect once, stop peeling when the stem tip is exposed, use a sterilized knife to cut a 1cm growth point, place it in 70% alcohol for 5 seconds, and then sterilize twice with 0.2% mercury liter solution, each time 5 Minutes, then rinsed with sterile water for 3 times, cut off the browned part, and set aside;

[0036] (3) Induced callus culture

[0037] Carry out callus culture with the above-mentioned explants to be used, ...

Embodiment 2

[0048] A method for banana tissue culture propagation, comprising the steps:

[0049] (1) Selection of explants

[0050] Select fruit-bearing 4 years, grow vigorously, and the banana head that does not have disease and pest is explant;

[0051] (2) disinfection of explants

[0052] Wash the above-mentioned banana head with clean water first, then use 70% alcohol to spray and disinfect, and then peel off the banana head under the condition of a sterile ultra-clean bench, and use 70% alcohol to clean after each layer of bracts is peeled off. Spray and disinfect once, stop peeling when the stem tip is exposed, use a sterilized knife to cut out a 2cm growth point, place it in 70% alcohol for 10 seconds, and then disinfect it twice with 0.2% mercury liter solution, each time 10 Minutes, then rinsed with sterile water 8 times, cut off the browned part, and set aside;

[0053] (3) Induced callus culture

[0054] Carry out callus culture with the above-mentioned explants to be use...

Embodiment 3

[0065] A method for banana tissue culture propagation, comprising the steps:

[0066] (1) Selection of explants

[0067] Select fruit-bearing 2 years, grow vigorously, and the banana head that does not have disease and pest is explant;

[0068] (2) disinfection of explants

[0069] Wash the above-mentioned banana head with clean water first, then use 70% alcohol to spray and disinfect, and then peel off the banana head under the condition of a sterile ultra-clean bench, and use 70% alcohol to clean after each layer of bracts is peeled off. Spray and disinfect once, stop peeling when the stem tip is exposed, use a sterilized knife to cut out a 1.5cm growth point, place it in 70% alcohol for 6 seconds, and then disinfect it twice with 0.2% mercury liter solution, each time After 6 minutes, rinse 4 times with sterile water, cut off the browned part, and set aside;

[0070] (3) Induced callus culture

[0071] Carry out callus culture with the above-mentioned explants to be use...

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Abstract

The invention discloses a banana tissue culture propagation method which is characterized by comprising the following steps: (1) explant selection; (2) explant disinfection; (3) inducement of callus culture; (4) differentiation culture; (5) strong seedling culture; (6) rooting culture; (7) hardening-seedling and transplanting. The banana tissue culture propagation method provided by the invention not only shortens the productive culture single cycle and the growth cycle and reduces the production occupation area, but also improves the survival rate of seedlings, thereby reducing capital investment, lowering production cost, and increasing profits of industrial production.

Description

technical field [0001] The invention belongs to the technical field of tissue culture production, and relates to a method for tissue culture propagation of bananas. Background technique [0002] Banana (Musa balbisiana Colla) is one of the important tropical fruits in southern my country, and it is also an important source of economic income in many areas, and many farmers get rid of poverty by growing bananas. Most cultivated edible bananas are parthenotriploid AAA, which have a high degree of sterility and mainly rely on asexual reproduction. In production, sucking buds are usually used for reproduction. When the seedlings are insufficient, the underground stems of bananas can also be cut into pieces. Propagate seedlings, but long-term use of this method will accumulate many viruses, such as: bunchy top disease, mosaic disease, etc. cause serious harm. In addition, poor banana seedlings can lead to poor growth, poor fruit, and inconsistent harvests. Banana tissue cultur...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 杨树东
Owner 杨树东
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