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Carrier-free immobilization aspergillus niger production method of isomaltose hypgather

A carrier-free immobilization and isomaltose oligosaccharide technology, applied in the field of oligosaccharide production, can solve the problems such as the inability to give full play to the advantages of immobilized cells, achieve less consumption of auxiliary materials, easy operation, and high α-glucosidase activity Effect

Active Publication Date: 2015-09-02
GUANGXI NANNING ZHITIAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the reported microbial-derived α-glucosidases are extracellular enzymes, and after the bacterial cells are immobilized, the secreted enzymes will still exist in the form of free enzymes, which cannot give full play to the advantages of immobilized cells.

Method used

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  • Carrier-free immobilization aspergillus niger production method of isomaltose hypgather

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: Method 1 of carrier-free immobilized Aspergillus niger producing isomaltooligosaccharide

[0020] 1. Utilize Aspergillus niger to prepare mycelia: use Aspergillus niger ( Aspergillus niger ) M1 is a fermentation strain. After culturing at 32°C for 36 hours, the fermentation broth was collected by vacuum filtration.

[0021] 2. Preparation of carrier-free immobilized cell packed column: the above-mentioned Aspergillus niger ( Aspergillus niger ) M1 bacterial cells and filter aid diatomaceous earth were mixed at a mass ratio of 1:1, stirred evenly, and then filled into the column according to 50% of the column volume to obtain a carrier-free immobilized cell-packed column.

[0022] 3. Production of isomaltooligosaccharide: Adjust the pH of 20% maltose syrup with a solid content of 20%, and add it to the above-mentioned carrier-free immobilized cell-packed column in a circular and continuous flow. The reaction temperature is 55°C, and the maltose syrup in ...

Embodiment 2

[0023] Embodiment 2: Method 2 of carrier-free immobilized Aspergillus niger producing isomaltooligosaccharide

[0024] 1. Utilize Aspergillus niger to prepare mycelia: use Aspergillus niger ( Aspergillus niger ) M1 is a fermentation strain. After culturing at 35°C for 36 hours, the fermentation broth was filtered through a plate frame to collect the bacteria.

[0025] 2. Preparation of carrier-free immobilized cell packed column: the above-mentioned Aspergillus niger ( Aspergillus niger ) M1 bacterial cells and filter aid diatomaceous earth were mixed at a mass ratio of 1:1, stirred evenly, and then filled into the column according to 50% of the column volume to obtain a carrier-free immobilized cell-packed column.

[0026] 3. Production of isomaltooligosaccharide: Adjust the pH of 30% maltose syrup with a solid content of 30%, and add it to the above-mentioned carrier-free immobilized cell-packed column in a circular and continuous flow. The reaction temperature is 60°C, a...

Embodiment 3

[0027] Embodiment 3: Method 3 of carrier-free immobilized Aspergillus niger producing isomaltooligosaccharide

[0028] 1. Utilize Aspergillus niger to prepare mycelia: use Aspergillus niger ( Aspergillus niger ) M1 is a fermentation strain. After culturing at 33°C for 40 hours, the fermentation broth was collected by centrifugation.

[0029] 2. Preparation of carrier-free immobilized cell packed column: the above-mentioned Aspergillus niger ( Aspergillus niger ) M1 bacterial cells are not mixed with any filter aid, and are directly loaded into the column according to 50% of the column volume to obtain a carrier-free immobilized cell-packed column.

[0030] 3. Production of isomaltooligosaccharide: adjust the pH of 10% maltose syrup with a solid content of 10%, and add it to the above-mentioned carrier-free immobilized cell-packed column in a circular and continuous flow. The reaction temperature is 50°C, and the maltose syrup in the packed column: The volume ratio of carri...

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Abstract

The invention discloses a carrier-free immobilization aspergillus niger production method of isomaltose hypgather. The method comprises the following steps: culturing aspergillus niger M1 and collecting mycelium, mixing the mycelium with a filter aid according to a proper weight ratio, uniformly stirring, filling a packed column reactor with the mixture to prepare a carrier-free immobilization cell packed column, and producing isomaltose hypgather having 35-70% of active trisaccharide (IG+P+IG3) by a fed-batch mode. By the use of aspergillus niger M1, produced alpha-glucosidase is endoenzyme. Thus, somatic cell is a natural carrier of enzyme, and there is no need to use other traditional organic or inorganic carriers for immobilization of thalline. In comparison with a traditional carrier immobilization cell technology, the carrier-free immobilization technology has advantages of simple technological operation, low auxiliary material consumption, no use of special equipment, long service life of somatic cell, high production intensity and the like.

Description

technical field [0001] The invention belongs to the technical field of oligosaccharide production, and in particular relates to a method for producing isomaltose oligosaccharide by carrier-free immobilized Aspergillus niger. Background technique [0002] Isomaltooligosaccharides (IMO) is a mixture of a series of glucans with a degree of polymerization of 2-10, all of which have at least one α-(1,6) glycosidic bond in their molecules. More components are isomaltose (Isomaltose, IG), panose (Panose, P) and isomaltotriose (Isomaltotriose, IG 3 ), these three components are called effective trisaccharides, and their content is the main index to evaluate the product quality of isomaltooligosaccharides, and is also a key factor affecting the product application range and market price. IMO is currently the most widely used and most demanded oligosaccharide functional sweetener. It has special physiological functions such as low calorie, anti-caries, and promotes the proliferation ...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12P19/12C12R1/685
Inventor 梁智群陈桂光李玮曾伟
Owner GUANGXI NANNING ZHITIAN BIOTECH
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