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A kind of method for producing isomaltose oligosaccharide by immobilizing Aspergillus niger without carrier

A carrier-free immobilization and isomaltose oligosaccharide technology, applied in the field of oligosaccharide production, can solve the problems such as the inability to give full play to the advantages of immobilized cells, and achieve the effects of less auxiliary material consumption, high bacterial concentration and easy operation.

Active Publication Date: 2018-02-13
GUANGXI NANNING ZHITIAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the reported microbial-derived α-glucosidases are extracellular enzymes, and after the bacterial cells are immobilized, the secreted enzymes will still exist in the form of free enzymes, which cannot give full play to the advantages of immobilized cells.

Method used

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  • A kind of method for producing isomaltose oligosaccharide by immobilizing Aspergillus niger without carrier

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: Method 1 of carrier-free immobilized Aspergillus niger producing isomaltooligosaccharide

[0020] 1. Utilize Aspergillus niger to prepare mycelia: use Aspergillus niger ( Aspergillus niger ) M1 is a fermentation strain. After culturing at 32°C for 36 hours, the fermentation broth was collected by vacuum filtration.

[0021] 2. Preparation of carrier-free immobilized cell packed column: the above-mentioned Aspergillus niger ( Aspergillus niger ) M1 bacterial cells and filter aid diatomaceous earth were mixed at a mass ratio of 1:1, stirred evenly, and then filled into the column according to 50% of the column volume to obtain a carrier-free immobilized cell-packed column.

[0022] 3. Production of isomaltooligosaccharide: Adjust the pH of 20% maltose syrup with a solid content of 20%, and add it to the above-mentioned carrier-free immobilized cell-packed column in a circular and continuous flow. The reaction temperature is 55°C, and the maltose syrup in th...

Embodiment 2

[0023] Embodiment 2: Method 2 of carrier-free immobilized Aspergillus niger producing isomaltooligosaccharide

[0024] 1. Utilize Aspergillus niger to prepare mycelia: use Aspergillus niger ( Aspergillus niger ) M1 is a fermentation strain. After culturing at 35°C for 36 hours, the fermentation broth was filtered through a plate frame to collect the bacteria.

[0025] 2. Preparation of carrier-free immobilized cell packed column: the above-mentioned Aspergillus niger ( Aspergillus niger ) M1 bacterial cells and filter aid diatomaceous earth were mixed at a mass ratio of 1:1, stirred evenly, and then filled into the column according to 50% of the column volume to obtain a carrier-free immobilized cell-packed column.

[0026] 3. Production of isomaltooligosaccharide: Adjust the pH of 30% maltose syrup with a solid content of 30%, and add it to the above-mentioned carrier-free immobilized cell-packed column in a circular and continuous flow. The reaction temperature is 60°C, and...

Embodiment 3

[0027] Embodiment 3: Method 3 of carrier-free immobilized Aspergillus niger producing isomaltooligosaccharide

[0028] 1. Utilize Aspergillus niger to prepare mycelia: use Aspergillus niger ( Aspergillus niger ) M1 is a fermentation strain. After culturing at 33°C for 40 hours, the fermentation broth was collected by centrifugation.

[0029] 2. Preparation of carrier-free immobilized cell packed column: the above-mentioned Aspergillus niger ( Aspergillus niger ) M1 bacterial cells are not mixed with any filter aid, and are directly loaded into the column according to 50% of the column volume to obtain a carrier-free immobilized cell-packed column.

[0030] 3. Production of isomaltooligosaccharide: adjust the pH of 10% maltose syrup with a solid content of 10%, and add it to the above-mentioned carrier-free immobilized cell-packed column in a circular and continuous flow. The reaction temperature is 50°C, and the maltose syrup in the packed column: The volume ratio of carrier...

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Abstract

The invention discloses a method for producing isomaltooligosaccharide by immobilizing Aspergillus niger without carrier. After collecting mycelium by cultivating Aspergillus niger M1, mixing it with filter aid in a suitable weight ratio, stirring evenly and loading Put it into a packed column reactor to make a carrier-free immobilized cell packed column, and produce isomaltooligosaccharide with an effective trisaccharide (IG+P+IG3) content of 35-70% by fed-batch method. Aspergillus niger (Aspergillus niger) M1 used in the present invention produces α-glucosidase as an intracellular enzyme, so the bacterial cell is the natural carrier of the enzyme, and there is no need to use other traditional organic or inorganic carriers to fix the bacterial cell change. Compared with the traditional carrier-immobilized cell technology, this carrier-free immobilization technology has the advantages of simple process operation, less consumption of auxiliary materials, no need for special equipment, long service life of bacterial cells, and high production intensity.

Description

technical field [0001] The invention belongs to the technical field of oligosaccharide production, and in particular relates to a method for producing isomaltose oligosaccharide by carrier-free immobilized Aspergillus niger. Background technique [0002] Isomaltooligosaccharides (IMO) is a mixture of a series of glucans with a degree of polymerization of 2-10, all of which have at least one α-(1,6) glycosidic bond in their molecules. More components are isomaltose (Isomaltose, IG), panose (Panose, P) and isomaltotriose (Isomaltotriose, IG 3 ), these three components are called effective trisaccharides, and their content is the main index to evaluate the product quality of isomaltooligosaccharides, and is also a key factor affecting the product application range and market price. IMO is currently the most widely used and most demanded oligosaccharide functional sweetener. It has special physiological functions such as low calorie, anti-caries, and promotes the proliferation ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14C12P19/12C12R1/685
Inventor 梁智群陈桂光李玮曾伟
Owner GUANGXI NANNING ZHITIAN BIOTECH