Method for acquiring concentration of antibiotics to restrain or kill target microorganisms

A technology of microorganisms and antibiotics, applied in biochemical equipment and methods, measurement/testing of microorganisms, enzymology/microbiology devices, etc. The formation is clearly visible, improving screening throughput and facilitating comparison
CN104878073AInactive Publication Date: 2015-09-02SUZHOU GENOARRAY CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SUZHOU GENOARRAY CO LTD
Publication Date
2015-09-02
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a method for acquiring concentration of to-be-measured antibiotics to restrain or kill target microorganisms. The method comprises the following steps: B1, covering a solid medium with a microchip, wherein the microchip is a film made of polydimethylsiloxane; B2, after the B1 is completed, adding liquid phases of to-be-measured antibiotics with the same volume and different concentrations in different through holes, and airing; B3, after the B2 is completed, adding the liquid phases containing the target microorganisms in the through holes, and culturing and observing the growth situation of the microorganisms so as to acquire the concentration of to-be-measured antibiotics to restrain or kill target microorganisms. The method has a very broad application prospect in evaluating the effect of the to-be-measured antibiotics to the target microorganisms or the study of screening antibiotics having an effect on the target microorganisms.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and specifically relates to a method for obtaining the concentration of antibiotics to inhibit or kill target microorganisms and a special device thereof, as well as the application of the method in evaluating the effect of the antibiotic to be tested on the target microorganisms and the application of the method in Application in screening for antibiotics effective against target microorganisms. Background technique

[0002] With the increase of bacterial resistance and the emergence of various new bacteria, people are constantly looking for new bacterial antibiotics. To judge whether a new compound or an isolated and extracted active ingredient has antibacterial effect, in vitro experiments are generally used, which need to be proved by pharmacological experiments, that is, to observe whether the tested substance can inhibit or kill bacteria. Drugs affect bacterial metabolism by reducing bacterial...

Claims

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