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Method for acquiring concentration of antibiotics to restrain or kill target microorganisms

A technology of microorganisms and antibiotics, applied in biochemical equipment and methods, measurement/testing of microorganisms, enzymology/microbiology devices, etc. The formation is clearly visible, improving screening throughput and facilitating comparison

Inactive Publication Date: 2015-09-02
SUZHOU GENOARRAY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the dilution method can grow the tested bacteria in a pure culture state, the results are reliable, but whether it is the broth dilution method or the agar dilution method, it is time-consuming and laborious to operate, and is not suitable for large-scale, rapid and efficient drug screening.

Method used

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  • Method for acquiring concentration of antibiotics to restrain or kill target microorganisms
  • Method for acquiring concentration of antibiotics to restrain or kill target microorganisms
  • Method for acquiring concentration of antibiotics to restrain or kill target microorganisms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1, the making of microchip

[0047] 1. Mask making

[0048] Use AotoCAD drawing software to design microchip graphics, convert the design graphics into graphics files, and use a high-resolution printer (≥1200dip) to print the graphics files on the film (Suzhou Meijing Micro Optoelectronics Co., Ltd. product), this film is the mask film, mask schematic diagram see figure 1 , 12 circular areas to print graphic files.

[0049] Compared with hard masks such as chromium plates commonly used in the microelectronics processing industry, the processing cost of film film is greatly reduced, and film film can meet the resolution requirements for structures above microns.

[0050] 2. Photolithography

[0051] Photolithography is the process of patterning silicon wafers or glass using light imaging and photosensitive adhesives.

[0052] Use acetone and isopropanol to thoroughly clean the silicon wafer (product of Jiangsu Intelsense Technology Co., Ltd.), dry it with ...

Embodiment 2

[0060] Embodiment 2, application of microchip to obtain the concentration of antimicrobials to be tested to inhibit or kill target microorganisms

[0061] 1. Preparation of medium and preparation of solid plates

[0062] LB liquid medium: solute and its concentration in the medium are: tryptone (Tryptone) 10g / L, yeast extract (Yeast extract) 5g / L, sodium chloride (NaCl) 10g / L; solvent is distilled water; The pH is 7.4±0.2. Sterilize at 121°C for 20 minutes with high pressure and damp heat.

[0063] LB solid medium: solute and its concentration are: tryptone (Tryptone) 10g / L, yeast extract (Yeast extract) 5g / L, sodium chloride (NaCl) 10g / L, agar powder (agar) 15g / L; The solvent is distilled water; PH is 7.4±0.2. Sterilize at 121°C for 20 minutes with high pressure and humidity.

[0064] LB solid plate: 10mL LB solid medium was sterilized at 121℃ for 20min by autoclaving and then lowered to 55℃, then poured into a sterile petri dish with a diameter of 90mm, then irradiated wit...

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Abstract

The invention provides a method for acquiring concentration of to-be-measured antibiotics to restrain or kill target microorganisms. The method comprises the following steps: B1, covering a solid medium with a microchip, wherein the microchip is a film made of polydimethylsiloxane; B2, after the B1 is completed, adding liquid phases of to-be-measured antibiotics with the same volume and different concentrations in different through holes, and airing; B3, after the B2 is completed, adding the liquid phases containing the target microorganisms in the through holes, and culturing and observing the growth situation of the microorganisms so as to acquire the concentration of to-be-measured antibiotics to restrain or kill target microorganisms. The method has a very broad application prospect in evaluating the effect of the to-be-measured antibiotics to the target microorganisms or the study of screening antibiotics having an effect on the target microorganisms.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a method for obtaining the concentration of antibiotics to inhibit or kill target microorganisms and a special device thereof, as well as the application of the method in evaluating the effect of the antibiotic to be tested on the target microorganisms and the application of the method in Application in screening for antibiotics effective against target microorganisms. Background technique [0002] With the increase of bacterial resistance and the emergence of various new bacteria, people are constantly looking for new bacterial antibiotics. To judge whether a new compound or an isolated and extracted active ingredient has antibacterial effect, in vitro experiments are generally used, which need to be proved by pharmacological experiments, that is, to observe whether the tested substance can inhibit or kill bacteria. Drugs affect bacterial metabolism by reducing bacterial...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18C12M1/00
Inventor 张函槊李娟庄峰锋
Owner SUZHOU GENOARRAY CO LTD
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