Process for preparing liquid protein feed by utilizing waste alcoholic fermentation liquor
A technology for fermenting waste mash and liquid protein with alcohol is applied in the field of preparing liquid protein feed, which can solve the problems of large sewage and serious energy consumption, and achieve the effects of reducing environmental pollution, reducing production costs and promoting growth and development.
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Embodiment 1
[0017] A process for preparing liquid protein feed using alcohol fermentation waste mash, the specific preparation steps are as follows:
[0018] (1) Activation of Aspergillus: Take fresh bran, use a 60-mesh sieve to remove fine powder, add water according to the ratio of bran: water = 1: 1.0-1.3, and mix well until there is no dry powder and no agglomeration. After mixing well, divide into 2000mL conical flasks, each 2000mL conical flask contains about 100g of wet bran, sterilize at 0.1MPa gauge pressure for 40-60min, cool to 35°C, put 1-2 Ring the activated spores of Aspergillus oryzae cv-8 and Aspergillus niger sp-1, and control the medium temperature at (35±1)℃. Shake the flask once every 12-24h, and stop preparing the spore suspension after the spores grow out;
[0019] (2) Activation of Candida utilis: Connect Candida utilis to YPD medium, shake the flask at 30°C and 180r / min for 24 hours; then transfer the seed solution to YPD at a ratio of 1:100 In the culture medium...
Embodiment 2
[0027] A process for preparing liquid protein feed using alcohol fermentation waste mash, the specific preparation steps are as follows:
[0028] (1) Activation of Aspergillus: Take fresh bran, use a 60-mesh sieve to remove fine powder, add water according to the ratio of bran: water = 1: 1.0-1.3, and mix well until there is no dry powder and no agglomeration. After mixing well, divide into 2000mL conical flasks, each 2000mL conical flask contains about 100g of wet bran, sterilize at 0.1MPa gauge pressure for 40-60min, cool to 35°C, put 1-2 Ring the activated spores of Aspergillus oryzae cv-8 and Aspergillus niger sp-1, and control the medium temperature at (35±1)℃. Shake the flask once every 12-24h, and stop preparing the spore suspension after the spores grow out;
[0029] (2) Activation of Candida utilis: Connect Candida utilis to YPD medium, shake the flask at 30°C and 180r / min for 24 hours; then transfer the seed solution to YPD at a ratio of 1:100 In the culture medium...
Embodiment 3
[0037] A process for preparing liquid protein feed using alcohol fermentation waste mash, the specific preparation steps are as follows:
[0038](1) Activation of Aspergillus: Take fresh bran, use a 60-mesh sieve to remove fine powder, add water according to the ratio of bran: water = 1: 1.0-1.3, and mix well until there is no dry powder and no agglomeration. After mixing well, divide into 2000mL conical flasks, each 2000mL conical flask contains about 100g of wet bran, sterilize at 0.1MPa gauge pressure for 40-60min, cool to 35°C, put 1-2 Ring the activated spores of Aspergillus oryzae cv-8 and Aspergillus niger sp-1, and control the medium temperature at (35±1)℃. Shake the flask once every 12-24h, and stop preparing the spore suspension after the spores grow out;
[0039] (2) Activation of Candida utilis: Connect Candida utilis to YPD medium, shake the flask at 30°C and 180r / min for 24 hours; then transfer the seed solution to YPD at a ratio of 1:100 In the culture medium,...
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