Condyloma acuminata animal model for photodynamic medicine screening

A technology of condyloma acuminata and animal models, applied in preparations for in vivo experiments, medical science, pharmaceutical formulas, etc., can solve the problems of high cost, low success rate, long modeling time, etc., and achieve high pathogenicity, The effect of fewer operations and shorter experiment time

Inactive Publication Date: 2015-09-09
陈志龙
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is that in order to overcome the poor controllability, instability, low success rate, long modeling time, complicated operation and high cost of establishing an animal model of hu

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 50 4-week-old SCID mice, weighing 11g±1-2g. The fresh wart tissue excised by outpatient surgery was placed in an ice box containing UW organ preservation solution and sent to the laboratory. After rinsing slightly with normal saline containing 100 U / mL of penicillin and streptomycin, the subcutaneous fat tissue of the wart tissue was completely removed. Steel ruler and vernier caliper measurement. No. 10 scalpel blade making 0.05cm 3 Several large and small tissue pieces were stuffed into the needle holes of the inoculation needle with tweezers, and inoculated subcutaneously on the back of the mouse. The whole experimental process was operated in strict accordance with the principle of aseptic surgery and was completed quickly. Always keep the wart tissue moist during the operation. Feed for two weeks. The mouse skin wounds gradually healed, and the wart tissue gradually bonded firmly with the skin tissue. Due to the influence of the blood supply, the moist and vi...

Embodiment 2

[0026] Two weeks after the inoculation, select 12 mice whose warts were all about 1 cm in diameter, and form a group of 6. The first group was the control group without treatment, and the second group was the treatment group. Apply photosensitizer 5-ALA emulsion on the surface of the affected area and within 1cm around it, and then evade light in a dark environment for 1 hour, and then irradiate with laser. A laser with a specific wavelength of 405nm is selected for irradiation. The irradiation area is determined according to the size of the wart, the irradiation time is about 30 minutes, and the irradiation dose is 150J / cm 2 . When the volume of the wart is large, the irradiation time should be extended appropriately and the irradiation points should be increased. After the PDT treatment by laser irradiation, the body weight of the mice all decreased within the normal range, and the mice appeared healthy. In the treatment group, various degrees of skin lesions, subcutaneou...

Embodiment 3

[0028] Two weeks after the inoculation, select 12 mice whose warts were all about 1 cm in diameter, and form a group of 6. The first group was the control group without treatment, and the second group was the treatment group. Apply 236 mg / ml of photosensitizer 5-ALA methyl ester suspension on the surface of the affected area and within 1 cm around it, and then evade light in a dark environment for 1 hour before laser irradiation. A laser with a specific wavelength of 405nm is selected for irradiation. The irradiation area is determined according to the size of the wart, the irradiation time is about 30 minutes, and the irradiation dose is 150J / cm 2 . When the volume of the wart is large, the irradiation time should be extended appropriately and the irradiation points should be increased. After the PDT treatment by laser irradiation, the body weight of the mice all decreased within the normal range, and the mice appeared healthy. In the treatment group, various degrees of sk...

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PUM

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Abstract

The invention discloses a condyloma acuminata animal model for photodynamic medicine screening and a building method of the condyloma acuminata animal model for photodynamic medicine screening. The building method includes the following steps: (1) fresh condyloma body tissues are sent to a laboratory, subcutaneous fatty tissues are thoroughly removed, and tissue blocks are manufactured, wherein the tissue blocks are measured through a steel ruler or a vernier caliper and are manufactured through a No.10 surgical knife blade, and the size of the tissue blocks ranges from 0.05 cm<3> to 0.1 cm<3>; (2) a male SCID mice which is four weeks old serves as a laboratory animal, and the single tissue blocks manufactured in the step (1) are plugged into a needle hole of an inoculating needle through tweezers so as to be inoculated under the skin of the back of the SCID mice; (3) the SCID mice inoculated in the step (2) is fed; and (4) the animal model fed in the step (3) is used for photodynamic medicine screening. The building method is short in laboratory time, high in HPV infection pathogenicity rate and low in modeling fatality rate; and the model can be applied to photodynamic treatment medicine screening for condyloma acuminate caused by human papilloma viruses.

Description

technical field [0001] The invention relates to the fields of microbiology, experimental zoology and photodynamic therapy, in particular to a condyloma acuminatum animal model used for photodynamic drug screening and a method for establishing the same. Background technique [0002] Human papillomavirus (HPV) is a double-stranded DNA virus belonging to the genus Papillomavirus. HPV infection or warts are common diseases in dermatology clinics. The infection site can spread all over the body, and both skin and mucous membranes can be involved. Genital warts are warty lesions on the genital area caused by HPV and are associated with genital cancer. Epidemiological studies have shown that the population carrier rate (including transient infection) can be as high as 50% to 60%. Its transmission is mainly through direct contact, can cause condyloma acuminata, etc., and is closely related to the occurrence of human tumors, especially cervical cancer and penile cancer. Therefore,...

Claims

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Application Information

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IPC IPC(8): A61K49/00A01K67/027A61D7/00
Inventor 陈志龙严懿嘉叶颖陈聃烨高迎华陈珍琰
Owner 陈志龙
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