Functional markers related to mutations in the coding region of Chinese cabbage cca1 gene and their application

Abstract

The invention discloses a functional marker relevant to the mutation of a coding region of a Chinese cabbage CCA1 gene. The functional marker comprises functional marker bodies respectively named as 5-InDel-W150 and 5-InDel-m156 relevant to insertion of 6bp for distinguishing 5'end and functional marker bodies respectively named as 3-InDel-W133 and 3-InDel-m139 relevant to insertion of 6bp for distinguishing 3'end, wherein the fragment size of the 5-InDel-W150 is 150bp, and the 5-InDel-W150 is as shown in SEQ ID No.1; the fragment size of the 5-InDel-m156 is 156bp, and the 5-InDel-m156 is as shown in SEQ ID No.2; the fragment size of the 3-InDel-W133 is 133bp, and the 3-InDel-W133 is as shown in SEQ ID No.3; and the fragment size of the 3-InDel-m139 is 139bp, and the 3-InDel-m139 is as shown in SEQ ID No.4. The functional marker provides an auxiliary selecting tool for culturing bolting-resistant Chinese cabbages and can also be used for identifying genetic resources of Chinese cabbages. Due to the application of the functional marker, the screening means can be greatly simplified, the transform breeding time can be shortened, and the selecting blindness in the conventional breeding method is avoided.

Description

technical field [0001] The invention belongs to the fields of vegetable breeding and molecular genetics, and specifically relates to two functional molecular markers related to the mutation of the CCA1 coding region, a key gene for photoperiod regulation in Chinese cabbage, and applications thereof. Background technique [0002] Chinese cabbage (Brassica rapa L. ssp pekinensis) is an important vegetable crop of the cruciferous family. It is native to China and is widely planted all over the world, especially in Southeast Asia. At present, new varieties of Chinese cabbage suitable for cultivation in different seasons of spring, summer and autumn have been bred, which has enabled the annual supply of Chinese cabbage, which has played a positive role in ensuring the supply of vegetables in the market and stabilizing prices. [0003] Among the Chinese cabbage varieties cultivated in different seasons, spring cabbage has the highest requirement for bolting resistance, mainly beca...

AI Technical Summary

Problems solved by technology

The detection method of InDel labeling depends on the size of the insert fragment. Large insert fragments (greater than 50bp) are generally separated by agarose gel, which is easy to operate, while small insert fragments (less than 30bp) are generally separated by polyacrylamide gel. Gel electrophoresis detection, the former is simple to operate, and the latter is cumbersome to operate

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