A microfluidic biochip detection device and preparation method

Abstract

The invention discloses a microfluidic biological chip detection device and a preparation method. The detection device is mainly composed of a groove plate, a bottom plate, a groove and a notch, and detection points are arranged in the groove. The detection device is simple to prepare and use, is suitable for large-scale production and application, and has stable and reliable detection results.

Description

technical field [0001] The invention relates to a microfluidic biochip detection device and a preparation method. Background technique [0002] At present, in vitro diagnostic product technologies on the market mainly include western blotting and enzyme-linked immunodiagnostic technology (ELISA), which detect the existence of corresponding antigens or antibodies through the interaction between antigens and antibodies, so as to achieve the purpose of diagnosing a certain disease. [0003] Western blotting is a protein identification technique widely used in the field of life sciences. The test requires steps such as electrophoresis, transfer, and detection. The operation steps are complicated, and only one serum can be used at a time to prevent contamination and interfere with the test. As a result, the device is prone to false negative results. [0004] ELISA is the most widely used immunoassay technique, which uses orifice plates as solid phase carriers to measure antigens...

AI Technical Summary

Problems solved by technology

[0006] Patent CN 101526520B discloses a detection method and device for biological samples. Although the detection of various indicators of multiple samples can be realized, the biomolecules are coated on the bottom plate. Whether it is sample preparation or detection, the first plate and the second plate The two plates cannot move relative to each other, otherwise the biomolecules on the second plate will be destroyed. Therefore, the requirements for the experiment are strict, and it is difficult to ensure the quality of the test.

In addition, when the device is used for sample preparation and testing, since the first flat plate has to be in motion, the position needs to be manually moved, which makes it difficult to keep the same position for sample preparation and testing, which makes it difficult to use the instrument to automatically check the test results during testing. for accurate reading

In addition, because the first plate has to be in motion and cannot be fixed, its application is limited, and it is only suitable for the temporary detection of a small number of samples in the laboratory, and cannot be used in industrial mass production; at the same time, because the first plate needs to be in the active state, it is difficult to achieve a sealed combination with the second plate, which makes it difficult to eliminate the cross-effect (contamination) of the sample liquid during sample preparation or detection, which affects the stability of the test results; and because the first plate needs to be in an active state, it is difficult to Avoid external influences on its prepared samples, so the detection conditions are strict, and it is required to be completed quickly in a short period of time from sample preparation to detection, so as to reduce the external influence on the stability of the test results

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