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Kit for detecting glycosylated hemoglobin and detection method thereof

A technology of glycosylated hemoglobin and a detection method, which is applied to a kit for detecting glycosylated hemoglobin and the detection field thereof, can solve the problems of being unsuitable for community and grass-roots hospitals, high cost of reagents and instruments, low accuracy, etc., so as to improve the screening speed, Simple operation and quick detection of the effect

Active Publication Date: 2015-09-09
GUANGDONG UNITY BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] To sum up, the several methods mentioned above are either expensive for reagents and instruments, or complicated to operate, or have low accuracy and poor stability, and are not suitable for communities and primary hospitals.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] A test kit for detecting glycosylated hemoglobin, said test kit comprising:

[0084] a first reagent comprising a borate derivative for lysing red blood cells, releasing glycosylated hemoglobin, and precipitating total hemoglobin;

[0085] a second reagent comprising a buffer for washing away unbound borate derivatives of glycated hemoglobin;

[0086] and a chromatograph including a reactive membrane for retaining hemoglobin precipitates.

[0087] The first reagent per liter comprises the following components:

[0088] Borate derivatives 0.4g

[0089] Magnesium chloride 1.9g

[0090] Potassium chloride 22g

[0091] Barium chloride 6.2g

[0092] Glycinamide hydrochloride 6.6g

[0093] Formamide 25g

[0094] Potassium azide 8g

[0095] Nonylphenol ethoxylate 15g

[0096] water balance.

[0097] The structural formula of the borate derivative is:

[0098] ,

[0099] Among them, R 1 is azinyl, R 2 is an alkyl group having 1 carbon atom.

[0100] The second ...

Embodiment 2

[0133] A test kit for detecting glycosylated hemoglobin, said test kit comprising:

[0134] a first reagent comprising a borate derivative for lysing red blood cells, releasing glycosylated hemoglobin, and precipitating total hemoglobin;

[0135] a second reagent comprising a buffer for washing away unbound borate derivatives of glycated hemoglobin;

[0136] and a chromatograph including a reactive membrane for retaining hemoglobin precipitates.

[0137] The first reagent per liter comprises the following components:

[0138] Borate derivatives 0.45g

[0139] Magnesium chloride 2.2g

[0140] Potassium chloride 23g

[0141] Barium chloride 6.7g

[0142] Glycinamide hydrochloride 7.2g

[0143] Formamide 28g

[0144] Potassium azide 9g

[0145] Nonylphenol ethoxylates 18g

[0146] water balance.

[0147] The structural formula of the borate derivative is:

[0148] ,

[0149] Among them, R 1 is azinyl, R 2 is an alkyl group having 2 carbon atoms.

[0150] The seco...

Embodiment 3

[0183] A test kit for detecting glycosylated hemoglobin, said test kit comprising:

[0184] a first reagent comprising a borate derivative for lysing red blood cells, releasing glycosylated hemoglobin, and precipitating total hemoglobin;

[0185] a second reagent comprising a buffer for washing away unbound borate derivatives of glycated hemoglobin;

[0186] and a chromatograph including a reactive membrane for retaining hemoglobin precipitates.

[0187] The first reagent per liter comprises the following components:

[0188] Borate derivatives 0.5g

[0189] Magnesium chloride 2.4g

[0190] Potassium chloride 24g

[0191] Barium chloride 7.2g

[0192] Glycinamide hydrochloride 7.6g

[0193] Formamide 30g

[0194] Potassium azide 10g

[0195] Nonylphenol ethoxylate 20g

[0196] water balance.

[0197] The structural formula of the borate derivative is:

[0198] ,

[0199] Among them, R 1 is azinyl, R 2 is an alkyl group having 3 carbon atoms.

[0200] The secon...

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Abstract

The invention relates to the technical field of detection of glycosylated hemoglobin and in particular relates to a kit for detecting glycosylated hemoglobin and a detection method thereof. The kit comprises a first reagent, a second reagent and a chromatograph, wherein the first reagent comprises a borate derivative used for splitting red cells, releasing the glycosylated hemoglobin and precipitating total hemoglobin; the second reagent comprises a buffer solution used for washing the borate derivative which is not combined with the glycosylated hemoglobin; and the chromatograph comprises a reaction film used for remaining hemoglobin precipitation. According to the kit disclosed by the invention, only trace whole blood live peripheral blood sample is needed, namely the content of the glycosylated hemoglobin can be quantitatively detected within 2-3 minutes, the screening speed is greatly improved, and the kit has the advantages of high sensitivity, high specificity, simplicity and convenience in operation and the like; and moreover, the preparation method is simple, and large-scale production is easily realized.

Description

technical field [0001] The invention relates to the technical field of glycosylated hemoglobin detection, in particular to a kit for detecting glycosylated hemoglobin and a detection method thereof. Background technique [0002] Diabetes mellitus is a group of endocrine and metabolic diseases whose etiology and pathogenesis are not yet fully understood, and its incidence rate is second only to cardiovascular diseases and tumors. In recent years, the incidence of diabetes has been on the rise, and it is a worldwide public health problem that seriously threatens human health. Traditional diabetes diagnosis and treatment monitoring uses fasting blood glucose, postprandial blood glucose and oral glucose tolerance test, etc., but blood glucose parameters only represent the instantaneous blood glucose level when blood is drawn. Recently, the detection of glycosylated hemoglobin (HbA1C) has been paid more and more attention in clinic. Glycosylated hemoglobin (HbA1C) refers to the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/72
CPCG01N33/723G01N33/726G01N2333/805G01N2800/042
Inventor 赖华李慧华梁伟业刘志文
Owner GUANGDONG UNITY BIOTECH
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