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New gloiopeltis tenax polysaccharide

A technology for polysaccharide and staghorn seaweed, which is applied in the directions of drug combination and antitumor drug, can solve the problems of complex polysaccharide advanced structure, lack of scientific basis and conclusion, and insufficient depth, etc., and achieves good application prospect and good antitumor activity. , good inhibitory effect

Inactive Publication Date: 2015-09-16
SHANTOU UNIV MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In recent years, the research on polysaccharides has made great progress, but compared with the research on proteins and nucleic acids, there is still a big gap, and it is not deep enough. The main problems are as follows: the structure-activity relationship of polysaccharides is not clear, and now It has been preliminarily believed that the biological activity of polysaccharides is related to its primary structure, advanced structure, molecular weight, solubility, etc., but there is a lack of regular scientific basis and conclusions, and most of them are speculation and analysis, while the solution conformation and crystal research of polysaccharides are almost blank.
[0005] The main reason is that the preparation, structure determination and synthesis of polysaccharides are relatively difficult. Since polysaccharides are not a single substance but a mixture with a certain molecular weight distribution, it is difficult to obtain their crystals; the primary structure of polysaccharides varies with the type of monosaccharide, Glycoside types, conformations, branching degrees, and substituents vary greatly, making it difficult to determine the primary structure, and the higher-level structure of polysaccharides is more complicated; these factors make polysaccharides more complex when they play a role Conformation studies, synthesis of polysaccharides and other aspects have caused great difficulties

Method used

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  • New gloiopeltis tenax polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Example 1 Extraction of Antler Antler Polysaccharide

[0064] 1. Extraction of crude polysaccharide (CP) from antler seaweed

[0065] Weigh 10g of Antler seaweed dry powder, treat it with about 20 times the volume of 80v / v% ethanol for 4h to degrease and remove the pigment, and centrifuge at 3000rpm for 10min in an oil bath at a temperature of 80°C and a solid-liquid ratio of 1:60 for 10min. Concentrate the supernatant at 55°C to 1 / 6 of the original volume, slowly add 3 times the volume of absolute ethanol while stirring, and overnight at 4°C; centrifuge at 3000rpm for 10min, take the precipitate, and wash with absolute ethanol, acetone, and anhydrous ether in sequence Washing; drying at 55°C until there is no ether smell, to obtain crude polysaccharide (CP) from Antler antler (CP), about 2.361g.

[0066] 2. Extraction of antler seaweed extract polysaccharide (RP)

[0067] Take 1g of antler seaweed crude polysaccharide (CP) and dissolve it in 100mL of distilled wate...

Embodiment 2

[0076] Embodiment 2 polysaccharide and protein content determination

[0077] 1. Determination of polysaccharide content of CP and RP by sulfuric acid-phenol method

[0078] 1) Make glucose standard curve: Accurately prepare 100μg / mL glucose standard solution, absorb 0.0, 0.1, 0.2, 0.4, 0.6, 0.8, 1.0, 1.2mL respectively, make up to 2mL with distilled water, then add 1mL of 6% phenol and 5mL of concentrated sulfuric acid, stand still for 10min, shake well, measure the absorbance at 490nm after standing at room temperature for 20min, 2mL of water is used as a blank according to the same color development operation, and operate in parallel three times. curve, as attached figure 1 shown.

[0079] 2) Determination of the polysaccharide content of the sample: draw 1.0mL of the sample solution, operate according to the above steps, measure the absorbance, and calculate the polysaccharide content with the standard curve. The results of polysaccharide content determination of CP a...

Embodiment 3

[0088] Example 3 Identification of the purity of the pure polysaccharide (PP) of antler seaweed

[0089] 1. The molecular weight of polymers can be counted by the following average molecular weights: number-average molecular weight (Mn), weight-average molecular weight (Mw), Z-average molecular weight (Mz), and viscosity-average molecular weight (M?). The relationship between weight, Z amount and the average molecular weight measured by the dilute solution viscosity method is (Mz) ≥ (Mw) ≥ (M?) ≥ (Mn), generally mainly depends on the weight average molecular weight (Mw).

[0090] As shown in Table 2, the polysaccharide molecular weight Mw calculated according to the linear equation LogM=9.7229-0.4316Te in the present invention is 7.72E+04.

[0091] Table 2 Relative average molecular weight of polysaccharide samples

[0092] mn mw Mz Mw / Mn 1.35E+04 7.72E+04 4.65E+05 5.74E+00

[0093] Note: * Generally, it mainly depends on Mw (weight average molec...

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Abstract

The invention discloses a new gloiopeltis tenax polysaccharide as well as a preparation method and application thereof. The preparation method disclosed by the invention comprises the following steps: firstly obtaining crude gloiopeltis tenax polysaccharide (CP) through a water-extraction and alcohol-precipitation method, performing enzymolysis and concentration so as to obtain fine gloiopeltis tenax polysaccharide (RP), and finally performing separation and purification through DEAE Sephrose CL-6B and Sephadex G-100 so as to obtain gloiopeltis tenax holosaccharide (PP). The gloiopeltis tenax polysaccharide disclosed by the invention mainly consists of glucose (Glc) and galactose (Gal), wherein the molar ratio of the glucose to the galactose is 1:1.86, the weight-average molecular weight is 7.72E+04 relative to the average molecular weight, and the specific structure consists of repetition structures of glucose-6-sulphuric acid base-beta-D-galactose (1->4)-3,6-inner ether-alpha-L-galactose (1->3). The gloiopeltis tenax polysaccharide disclosed by the invention has good antineoplastic activity, and has good application prospects in the respect of preparing antitumor drugs.

Description

technical field [0001] The invention belongs to the field of pharmacy. More specifically, it relates to an antler antler polysaccharide and its preparation method and application. Background technique [0002] Seaweed polysaccharide is a kind of mixture, which is the general name of various high molecular carbohydrates contained in the intercellular and intracellular of seaweed. It is rich in seaweed, accounting for about 20-70% of the dry weight. Depending on the source of seaweed, it can be divided into fucoidan, red algae polysaccharide, and chlorella polysaccharide. More and more studies have proved that polysaccharides have complex and multifaceted biological activities and functions, especially the effect on the immune function of the body. [0003] Gloiopeltis furcata, Rhodophyta, Florideophyceae, Cryptonemiales, Endocladiaceae, Gloipeltis J.Agardh, commonly known as red cabbage, is Annual red algae. Researchers at home and abroad have successively found that carn...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P35/00
Inventor 石刚刚陈一村江泽斌郑娇娇
Owner SHANTOU UNIV MEDICAL COLLEGE