Method for Inducing Callus Formation from Phyllostachys pubescens Seed Embryo

Abstract

A phyllostachys pubescens embryo induced callus formation method comprises the following steps: (1) screening out phyllostachys pubescens seeds with the vitality higher than 75%; (2) taking out embryos of the screened phyllostachys pubescens seeds, cutting to remove 50% or more than 50% of endosperm, conducting treatment by use of running water, ethanol, bacteria-free water, a sodium hypochlorite solution, bacteria-free water, mercury bichloride added with Tween-80 in sequence, and then removing surface moisture for later use; (3) inoculating disinfected embryos onto an induction medium, conducting dark culture for 7-10 days to form calluses, then separating the calluses out, inoculating the separated calluses onto a propagation medium for propagation culture for 20-30 days. According to the phyllostachys pubescens embryo induced callus formation method, as the embryos of the phyllostachys pubescens seeds are selected as explants, dirt, clay, bacteria and other pollutants, hidden in gaps between the testa and internal embryos of the phyllostachys pubescens seeds can be directly removed, and accordingly, the pollution rate is effectively reduced. The phyllostachys pubescens embryo induced callus formation method has the advantages that the callus inductivity can reach 64.3%, the pollution rate can be controlled at 20% or below, and the calluses are favorable in condition and excellent in growth situation.

Description

technical field

[0001] The invention relates to the fields of tree biotechnology and tissue culture, in particular to a method for inducing callus formation by moso bamboo seed embryos. Background technique

[0002] Moso bamboo (Phyllostachys Pubescens), also known as Nanzhu, Mengzong bamboo, Jiangnan bamboo, belongs to Gramineae (Gramineae), Bambusoideae (Bambusoideae), Phyllostachys (Phyllostachys) plants, perennial evergreen scattered bamboo plants, is a traditional Chinese Bamboo species are sold, which can be used for wood, food, ornamental and many other purposes. It also has the function of fixing soil and wind. It is widely used in building materials, construction, green food, medical care, furniture and agricultural tools, daily necessities, tourism handicrafts, cultural, sports and cultural equipment and the environment. Landscaping and other fields.

[0003] The national moso bamboo forest is about 3 million ha 2 , accounting for about 65% of the total area of ​...

Images