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Nanometer gold spherical shell photosensitive liposome and preparation method for same

A liposome and nano-gold technology, which is applied in liposome delivery, pharmaceutical formulations, medical preparations of non-active ingredients, etc., can solve the problems that are difficult to act on deep tissue sites, side effects of cells and tissues, and dependence on ultraviolet light irradiation, etc. problems, to achieve the best light-to-heat conversion performance, easy control of the reaction, and the effect of improving tissue penetration ability

Active Publication Date: 2015-09-23
YANSHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] But the shortcoming of above-mentioned photosensitive liposome is: it needs to rely on ultraviolet light to irradiate, just can reach the effect of treatment
The wavelength range of ultraviolet light is 10-380nm, which not only has poor tissue penetration ability, it is difficult to act on deep tissue sites, but also has great side effects on normal cells and tissues of the human body

Method used

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  • Nanometer gold spherical shell photosensitive liposome and preparation method for same
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  • Nanometer gold spherical shell photosensitive liposome and preparation method for same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1 mg wedelolide (purchased from Shanghai Zhanshu Chemical Technology Co., Ltd.), 55 mg soybean lecithin (purchased from Shenyang Tianfeng Biopharmaceutical Co., Ltd.) and 5 mg cholesterol (purchased from Tianjin Damao Chemical Instrument Supply Station) were dissolved in In 4mL absolute ethanol, a homogeneous oil phase, ie lipid ethanol solution, was obtained by magnetic stirring. The liposome ethanol solution was dropped dropwise into 10mL ultrapure water at 37°C, and a magnetic stirrer was used to stir slowly throughout the dropping process to obtain a liposome suspension. By means of rotary evaporation or magnetic stirring, at 25° C., the absolute ethanol in the liposome suspension was removed at a uniform speed and slowly to obtain a wedelide nanoliposome solution.

[0026] The configuration concentration is the cysteine ​​hydrochloride solution of 0.5 mg / mL, and 2 mL of the wedelia lactone nanoliposome solution obtained before is added dropwise in 1 mL of cysteine ...

Embodiment 2

[0031] 1 mg wedelolide (purchased from Shanghai Zhanshu Chemical Technology Co., Ltd.), 60 mg soybean lecithin (purchased from Shenyang Tianfeng Biopharmaceutical Co., Ltd.) and 6 mg cholesterol (purchased from Tianjin Damao Chemical Instrument Supply Station) were dissolved in In 4mL absolute ethanol, a homogeneous oil phase, ie lipid ethanol solution, was obtained by magnetic stirring. Take 2mL lipid ethanol solution and drop it into 10mL ultrapure water at 40°C. During the dropping process, a magnetic stirrer is used to stir slowly throughout the whole process to obtain a liposome suspension. By means of rotary evaporation or magnetic stirring, at 28° C., the absolute ethanol in the liposome suspension was removed at a uniform speed and slowly to obtain a wedelide nanoliposome solution.

[0032] The cysteine ​​hydrochloride solution with a concentration of 1.0 mg / mL is configured, and 2 mL of the previously obtained wedelolactone nanoliposome solution is added dropwise to 1...

Embodiment 3

[0037] 1 mg wedelolide (purchased from Shanghai Zhanshu Chemical Technology Co., Ltd.), 65 mg soybean lecithin (purchased from Shenyang Tianfeng Biopharmaceutical Co., Ltd.) and 6 mg cholesterol (purchased from Tianjin Damao Chemical Instrument Supply Station) were dissolved in In 4mL absolute ethanol, a homogeneous oil phase, ie lipid ethanol solution, was obtained by magnetic stirring. Take 3mL lipid ethanol solution and drop it into 10mL ultrapure water at 43°C. During the dropping process, a magnetic stirrer is used to stir slowly throughout the whole process to obtain a liposome suspension. By means of rotary evaporation or magnetic stirring, the absolute ethanol in the liposome suspension was removed at a uniform speed and slowly at 30° C. to obtain a wedelide nanoliposome solution.

[0038] The cysteine ​​hydrochloride solution with a concentration of 2.0 mg / mL is configured, and 2 mL of the previously obtained wedelolactone nanoliposome solution is added dropwise to 1 ...

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Abstract

The invention discloses nanometer gold spherical shell photosensitive liposome, which consists of a conventional drug liposome and a nanometer gold spherical shell structure. A preparation method for the photosensitive liposome mainly comprises the following steps of first combining cysteine to a liposome surface wrapped with wedelolactone to make the surface rich in sulphydryl groups; then reducing gold chloride to obtain nanometer gold particles by virtue of a reducing agent sodium borohydride; finally mixing sulphydryl-modified liposome and the nanometer gold particles according to a certain proportion, and performing incubation under a specific condition to obtain the nanometer gold spherical shell photosensitive liposome. The preparation method can be implemented under normal temperature, normal pressure and a mild condition, a process is simple, reaction is easy to control, and the obtained liposome is regular in shape, has particle size distribution of 200 to 250nm and high light-heat conversion performance, and can be used for effectively absorbing near infrared light of 700 to 850nm.

Description

[0001] Technical Field The present invention belongs to the field of new materials, in particular to a photosensitive material and a preparation method thereof. Background technique [0002] The birth of nanoliposomes has created a new era for drug delivery, and how to use this platform better and more efficiently has become a new topic in the field of scientific research. The emergence of photosensitive materials has brought new opportunities for the development of new liposome drug dosage forms. It combines traditional chemotherapy technology with emerging hyperthermia technology, providing a new means for the treatment of tumor diseases. [0003] As a photosensitive material, gold nanoparticles have good biocompatibility and good photothermal conversion ability, and are widely used in medical diagnosis. Studies have shown that monodisperse gold nanoparticles have strong surface plasmon resonance (SPR) absorption in the ultraviolet spectral region. However, by reducing the...

Claims

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Application Information

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IPC IPC(8): A61K9/127A61K41/00A61K31/37A61K47/24A61K47/28A61P35/00
Inventor 高大威李楠张旭武刘艳平边艳红籍冰朔
Owner YANSHAN UNIV
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