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L-tryptophan refining method

A refining method and tryptophan technology, applied in the refining field of L-tryptophan, can solve the problems of reduced product yield and purity, low tryptophan solubility, large amount of organic solvent, etc. The effect of saving solvent and increasing stability

Inactive Publication Date: 2015-09-23
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage is that the amount of organic solvent added is relatively large, and steam needs to be consumed during recycling. In addition, the addition of organic solvents during the crystallization process may also cause the transformation of the crystal form.
The disadvantage is that the solubility of tryptophan in water is very low (solubility at 25°C is 1.136%, solubility at 75°C is 2.795%), a large amount of steam is consumed when concentrated crystallization is used, and long-term vacuum concentration in aqueous solution will cause L-tryptophan to decompose , resulting in lower product yield and purity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 20g of crude tryptophan (purity 80.21%) was dissolved in 100mL of 75% acetic acid aqueous solution, and the temperature was raised to 95°C to dissolve it. Add 1 g of anhydrous Na to the solution 2 SO 3 , 4M sodium hydroxide solution to adjust the pH to 2.0. According to the proportion of 15% of the mass of the crude product, add 3g of activated carbon and keep stirring to decolorize for 0.5h. The decolorization temperature is 75°C. After rapid suction filtration, cool and crystallize at a cooling rate of 10°C / h within 3h, and then cool down to 4°C for cooling and crystallization within 2h. The crystals were collected by suction filtration and dried. A total of 13.73g of crystals, a purity of 99.6%, and a crystallization yield of 92.2%.

Embodiment 2

[0027] 20g of crude tryptophan (purity 87.4%) was dissolved in 100mL of 80% acetic acid aqueous solution, and the temperature was raised to 60°C to dissolve it. Add 5 g of anhydrous Na to the solution 2 SO 3 , 4M sodium hydroxide solution to adjust the pH to 5.0. According to the proportion of 20% of the mass of the crude product, add 4g of activated carbon and keep stirring to decolorize for 0.5h. The decolorization temperature is 95°C. After rapid suction filtration, cool and crystallize at a cooling rate of 10°C / h within 3h, and then cool down to 20°C at a uniform rate within 2h for cooling and crystallization. The crystals were collected by suction filtration and dried. The crystals totaled 15.07g, the purity was 99.7%, and the crystallization yield was 94.3%.

Embodiment 3

[0029] 20g of crude tryptophan (purity 77.37%) was dissolved in 100mL of 50% acetic acid aqueous solution, and the temperature was raised to 80°C to dissolve it. Add 1 g of anhydrous NaHSO to the solution 3 , 4M sodium hydroxide solution to adjust the pH to 2.5. According to the proportion of 15% of the mass of the crude product, add 3g of activated carbon and keep stirring to decolorize for 0.5h. The decolorization temperature is 65°C. After rapid suction filtration, cool and crystallize at a cooling rate of 10°C / h within 3h, and then cool down to 15°C at a uniform rate within 2h for cooling and crystallization. The crystals were collected by suction filtration and dried. A total of 14.85 g of crystals were obtained, the purity was 99.5%, and the crystallization yield was 95.2%.

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Abstract

The invention provides an L-tryptophan refining method. The method comprises the steps of dissolving crude products in a water and acetic acid mixed solvent; adding an antioxidant, and regulating the pH value; adding activated carbon for decoloration, and then conducting filtration; conducting cooling crystallization, centrifugal collection of crystals and drying to obtain L-tryptophan. The purity of L-tryptophan refined with the method is not lower than 99.5%. Compared with a vacuum concentration recrystallization refining method, the method has the advantages that steam consumption is reduced greatly, and yield each time is increased by over 50%.

Description

Technical field [0001] The invention involves a refined method of a L-chromine, which is a biochemical field. Background technique [0002] L-chromine name β-吲哚lanine, relative molecular weight 204.23.It is white or slightly yellow -like crystals or powder, without odor or slight odor, dissolved in hot pyridine, slightly soluble in ethanol, and insoluble in chloroform and ether.Solk in dilute acid or dilute alkali, and long -term light is colorful.Together with water to produce a small amount, such as in NaOH or CUSO 4 If there is heating down, a large number of crickets are generated.Heating in the dark is unstable.It is more stable in the alkali solution, but it is easy to decompose when other amino acids or sugar. [0003] L-chromine is one of the essential amino acids in human and animal life activities. It plays an important role in the growth and development of human and animals, and is widely used in medicine, food and feed.L-chromine is a relatively lack of amino acids in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D209/20
CPCC07D209/20
Inventor 吴伟斌黄建忠郭小雷黄平吴松刚
Owner FUJIAN NORMAL UNIV
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