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A three-inlet concentration gradient generator and a method for generating a power function concentration gradient

A concentration gradient and generator technology, applied in the application field, can solve problems such as poor versatility, increased requirements for external instruments, complex equipment structure, etc., to achieve the effect of simplified structure and simple and compact overall structure

Active Publication Date: 2017-09-29
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Relatively speaking, the structure of the convective concentration gradient generator is more complicated, requiring a long straight channel or a micro-mixer
In addition, generally a single concentration gradient generator can only be used to generate a concentration gradient, that is, the versatility of the equipment is poor
In the current structure design of the concentration gradient device, if a variety of concentration gradients are to be realized on a single device, many fluid inlets need to be set up, which makes the device structure very complicated and greatly increases the requirements for external instruments

Method used

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  • A three-inlet concentration gradient generator and a method for generating a power function concentration gradient
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  • A three-inlet concentration gradient generator and a method for generating a power function concentration gradient

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preparation example Construction

[0034] The preparation method of the concentration gradient generator is as follows:

[0035](1) Photolithography: Design and draw the micro-channel pattern, and then output the pattern to the film. Using standard photolithography technology, transfer the micro-channel pattern to the silicon substrate template of SU-8 negative photoresist. forming a male mold patterned by microchannels;

[0036] (2) Pattern transfer: Mix the PDMS prepolymer and cross-linking agent evenly according to the mass ratio of 10:1, pour horizontally onto the SU-8 mold obtained by photolithography, put it in a vacuum box for 15 minutes to remove air bubbles, and then put Heat it in an oven at 70°C for 2 hours until it solidifies and take it out. Cut and peel off the PDMS, then use a hole punch to punch holes at the individual inlet and outlet channels;

[0037] (3) Bonding: The PDMS substrate with microstructure and the cleaned and dried glass cover slip are permanently bonded with a plasma machine t...

Embodiment 1

[0039] Example 1: Generation of a linear concentration gradient

[0040] (1) Preparation: prepare 5 mg / L and 2.5 mg / L fluorescein isothiocyanate (FITC) aqueous solutions respectively. Connect the three inlets to the three syringe pumps, and the outlet channel to the waste liquid pool.

[0041] (2) Operating parameters: inject 5mg / L FITC solution into the first inlet, inject 2.5mg / L FITC solution into the second inlet, and inject deionized water into the third inlet. The flow rate at the second inlet is 20 μm / s. For a concentration gradient generator with a width of 400 μm and a height of 30 μm, the width of the second inlet is 168 μm, and the flow rate of the second inlet can be calculated to be 6.05 nL / min. According to the aforementioned relationship of r=0.95-0.41lna, where a=1, r=0.95 can be obtained, which is approximately 1, that is, the flow rates of the three inlet channels are equal. The widths of inlet 1 and inlet 3 are both 106 μm, and the flow rates of inlet 1 an...

Embodiment 2

[0043] Embodiment 2: y=x 2 Generation of Concentration Gradients

[0044] (1) Preparation: prepare 5 mg / L and 1.25 mg / L fluorescein isothiocyanate (FITC) aqueous solutions respectively. Connect the three inlets to the three syringe pumps, and the outlet channel to the waste liquid pool.

[0045] (2) Operating parameters: inject 5 mg / L FITC solution into the first inlet, inject 1.25 mg / L FITC solution into the second inlet, and inject deionized water into the third inlet. Inlet two flow is still 6.05nL / min. According to the aforementioned relationship of r=0.95-0.41lna, where a=2, r=0.67 can be obtained, that is, the speed of the third entrance is 13.2 μm / s. From this, the flow velocity of inlet one can be obtained as 26.8 μm / s. The widths of the first and third inlets are both 106 μm, and the flow rate of the first inlet is 5.11 nL / min, and the flow rate of the third inlet is 2.52 nL / min.

[0046] (3) Acquisition of results: Place the concentration gradient generator unde...

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Abstract

The invention discloses a three inlet concentration gradient generator and a power function concentration gradient generation method. The concentration gradient generator comprises three inlets, an outlet, a main passage and a series of flow distribution plates parallel to the main channel, and is prepared through a soft lithography method. The area where the flow distribution plates are located is a mixing area with fluid in different concentrations, and comprises three stages, wherein the first stage of the area comprises two flow distribution plates, the second stage of the area comprises four flow distribution plates, and the third stage of the area comprises eight flow distribution plates; the part, at the downstream of the flow distribution plates, of the main passage is the formation area of concentration gradient. Through control of flow and concentration of the three inlets, establishment of concentration gradient of different target power functions is realized. The concentration gradient generator is compact in structure, adjustable in concentration gradient and simple and feasible in adjustment method, and can be widely applied to the fields of cell directed migration, cell culture and the like in biology, pharmacy, medical engineering and the like.

Description

technical field [0001] The invention relates to a microfluidic chip and its application fields such as biology, pharmacy, and medical engineering, and in particular to a microfluidic-based concentration gradient generator and a method for generating a power function concentration gradient. Background technique [0002] The migration of cells, the directional growth of neuron axons, and the chemotaxis of microorganisms are all affected by the concentration gradient of certain chemical substances. Therefore, realizing a controllable concentration gradient has important practical value in cell biology research, drug screening and medical engineering. Microfluidic devices are widely used to generate concentration gradients because of their small size, precise laminar flow, and effective control of fluid mixing. [0003] The current microfluidic concentration gradient generators mainly include two types: diffusion type and convection type. The diffusion type uses the free diffu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00
CPCC12M23/16
Inventor 许忠斌黄兴崔赟
Owner ZHEJIANG UNIV
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