A kind of Paecilomyces lilacinus and its large-scale preparation method

A Paecilomyces lilacinus, large-scale technology, applied in the field of Paecilomyces lilacinus and its large-scale preparation, can solve the problems of low spore production, low fermentation efficiency and long production cycle of Paecilomyces lilacinus strains, and achieve the goal of inoculation And the effect of high fermentation efficiency, simple preparation method and low preparation cost

Active Publication Date: 2018-07-27
湖北龙翔生物制品发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the prior art, for example, the publication number is the patent of CN 10332032, which adopts the solid-liquid two-phase method to ferment, and its spore content is only 10 9 cfu / g, and as the publication number CN 102925405 adopts the mode of liquid state fermentation to carry out the large-scale production of Paecilomyces lilacinus strain, its viable spore amount is also only 10 9 cfu / g, the fermentation efficiency is generally low
In addition, the existing large-scale production method of Paecilomyces lilacinus has a long production cycle. For example, the publication number is: CN 103589649 adopts the method of solid-state culture, and the production cycle is long, reaching 32-37 days, which cannot meet the requirements for the production of Paecilomyces lilacinus. the actual needs of
[0005] In summary, the existing Paecilomyces lilacinus strains have low spore production, lack of suitable large-scale fermentation methods, and cannot meet the needs of large-scale control of root-knot nematodes.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Liquid fermentation: 5g of corn flour and 1000ml of water, mix and boil for 8 minutes, add 10g of sugar to make a liquid fermentation medium, inoculate the bacterium block of Paecilomyces lilacinus with the preservation number of CGMCC NO.5339, in 25 Shake in a shaker at ℃, the shaker speed is 200rpm, and shake for 3 days to obtain liquid seed solution A;

[0028] 2. Sterilization of solid-state fermentation substrates: The solid-state fermentation substrates are mixed according to mass ratio: 500 parts of the mixture of main substrate wheat bran and bean cake, 300 parts of auxiliary substrate corn flour, 200 parts of millet flour, 5 parts of shrimp powder, and 20 parts of ammonium sulfate. Evenly, the solid fermentation substrate is sterilized by injecting 100°C steam into the solid fermentation substrate by intermittent steam, the sterilization time is 30min, and the interval is 18 hours, and repeated 3 times to obtain the sterilized solid fermentation substrate B; ...

Embodiment 2

[0035] 1. Liquid fermentation: 12g of corn flour and 1000ml of water, mix and boil for 15 minutes, add 20g of sugar to make a liquid fermentation medium, inoculate the bacterial block of Paecilomyces lilacinus with the preservation number of CGMCC NO.5339, in 30 Shake in a shaker at ℃, the shaker speed is 180rpm, and shake for 5 days to obtain liquid seed solution A;

[0036]2. Sterilization of solid-state fermentation substrates: The solid-state fermentation substrates are mass-proportioned: 800 parts of the main substrate wheat bran, 450 parts of a mixture of auxiliary substrate corn flour and yeast powder, 50 parts of millet flour, 50 parts of shrimp powder, and 10 parts of ammonium sulfate. Mix well, and use intermittent steam to pass the solid fermentation substrate into 100°C steam for sterilization. The sterilization time is 40 minutes, and the interval is 24 hours. Repeat 4 times. After the sterilization, the sterilized solid fermentation substrate B is obtained;

[00...

Embodiment 3

[0043] 1. Liquid fermentation: Mix 8 g of corn flour and 1000 ml of water, boil for 12 minutes, add 15 g of sugar to make a liquid fermentation medium, and inoculate the bacterium block of Paecilomyces lilacinus with the preservation number of CGMCC NO.5339. Shake in a shaker at ℃, the shaker speed is 160rpm, and shake for 5 days to obtain liquid seed solution A;

[0044] 2. Sterilization of the solid-state fermentation substrate: the solid-state fermentation substrate according to the mass ratio: 650 parts of main substrate bean cake, 375 parts of auxiliary substrate yeast powder, 125 parts of millet flour, 30 parts of shrimp powder, 1 part of ammonium sulfate, mix well, and use intermittent steam The method is to pass the solid fermentation substrate into 100°C steam for sterilization, the sterilization time is 30 minutes, and the interval is 21 hours, repeating 5 times to obtain the sterilized solid fermentation substrate B;

[0045] 3. Solid matrix spray inoculation: Dilut...

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Abstract

The invention discloses paecilomyces lilacinus and a scale preparation method thereof, belongs to the technical field of microbial agricultural pesticide fertilizer, and particularly relates to a bacterial strain of the paecilomyces lilacinus of which the preservation number is CGMCC (China General Microbiological Culture Collection Center) No. 5339. The scale preparation of the paecilomyces lilacinus is realized by adopting a way of combining liquid state fermentation with solid state fermentation for the strain, namely, through four steps of the liquid state fermentation, the sterilization of a solid state fermentation substrate, the spray inoculation of the solid state fermentation substrate, and the culture of the substrate after the inoculation. The method has the advantages of high fermentation efficiency, short preparation period, simple preparation method and low preparation cost, the wide application of the paecilomyces lilacinus to agricultural disease control can be realized, and the actual demands of the high-efficiency and scale control of nematodes can be met.

Description

Technical field: [0001] The invention belongs to the technical field of agricultural medicinal fertilizers for microorganisms, and in particular relates to Paecilomyces lilacinus and a large-scale preparation method thereof. Background technique: [0002] Root-knot nematode is a widely distributed and serious plant parasitic nematode, which causes great economic losses to agricultural and forestry production. Root-knot nematodes can directly damage the epidermal cells of the host to cause mechanical damage to the crops, and can also stab the host through the stylet, secrete toxic substances, form nodular root knots, and affect the growth of crops. [0003] As a high-efficiency microbial fertilizer, Paecilomyces lilacinus can effectively control root-knot nematode damage. However, in practical applications, many of the existing microbial strains are not as effective as chemical nematicides due to their limited spore content. Therefore, how to expand the spore production of P...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/79
CPCC12N1/14
Inventor 陈育金雪菲党国瑞梁兴慧毛爱华杜进平
Owner 湖北龙翔生物制品发展有限公司
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