A method for preparing protein hydrogel microspheres using bovine serum albumin as a base inverse microemulsion

A technology of bovine serum albumin and hydrogel microspheres, which is applied in the field of biological tissue medical material preparation, can solve the problems of organism infection, poor filling surface morphology, and the shape of hydrogel cannot meet the ideal requirements, and achieves the biological phase. Good capacitance, optimized surface morphology of material filling, and the effect of solving poor morphology

Active Publication Date: 2017-04-12
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to provide a method for preparing protein hydrogel microspheres with bovine serum albumin as the base inverse microemulsion, which solves the problem that the shape of the hydrogel cannot be achieved due to its large volume during use. Ideal requirements, and materials in soft tissue filling are easy to cause biological infection, filling surface morphology is not good and other shortcomings; and protein hydrogel microspheres have better biocompatibility, after being prepared into microspheres, they can be used in tissue engineering materials, It has broad application prospects in drug sustained release and cosmetics

Method used

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  • A method for preparing protein hydrogel microspheres using bovine serum albumin as a base inverse microemulsion
  • A method for preparing protein hydrogel microspheres using bovine serum albumin as a base inverse microemulsion
  • A method for preparing protein hydrogel microspheres using bovine serum albumin as a base inverse microemulsion

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Embodiment 1

[0031] During the preparation process, 1 g of hexamethylenediamine was weighed to make 25 ml of aqueous solution, and the pH of the solution was adjusted to 6.5 with concentrated hydrochloric acid. Then slowly add the solution dropwise to 200mg of bovine serum albumin (BSA), add 100mg of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and stir for 10h. Aminated BSA is finally obtained. Then use 40ml dimethyl sulfoxide as solvent to dissolve 2g dextran, 1g p-carboxybenzaldehyde, 4-dimethylaminopyridine to form a solution, and then add 1.2g dicyclohexylcarbodiimide in the solution to start the reaction, 20 Reaction at ℃ for 18h, after post-treatment to obtain multifunctional dextran. Add 5 μl of co-emulsifier, 15 μl of 150 mg / ml Triton X-100 (emulsifier), 15 μl of aminated BSA, 15 μl of 0.05M buffer pH=7.5, 15 μl of difunctionalized dextran into the container, and then add 400 μl After the oil phase was shaken for 30 s, protein hydrogel microspheres were obta...

Embodiment 2

[0033] During the preparation process, 1 g of hexamethylenediamine was weighed to make 25 ml of aqueous solution, and the pH of the solution was adjusted to 6.5 with concentrated hydrochloric acid. Then slowly add the solution dropwise to 200mg of bovine serum albumin (BSA), add 100mg of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and stir for 10h. Aminated BSA is finally obtained. Then use 40ml dimethyl sulfoxide as solvent to dissolve 2g dextran, 1g p-carboxybenzaldehyde, 4-dimethylaminopyridine to form a solution, and then add 1.2g dicyclohexylcarbodiimide in the solution to start the reaction, 20 Reaction at ℃ for 18h, after post-treatment to obtain multifunctional dextran. Add 5 μl of co-emulsifier, 15 μl of 100 mg / ml Triton X-100 (emulsifier), 15 μl of aminated BSA, 15 μl of 0.05M buffer pH=7.5, 15 μl of polyfunctionalized dextran into the container, and then add 400 μl After the oil phase was shaken for 30 s, protein hydrogel microspheres were ob...

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Abstract

The invention discloses a method for preparing protein hydrogel microspheres by taking bovine serum albumin as element reversed-phase microemulsion. The method comprises the following steps: 1, weighing hexamethylene diamine and preparing into a solution, regulating the pH of the solution to the range of 6 to 7.5, next, slowly and dropwise adding the solution to the BSA, adding EDC for reacting, and finally obtaining aminated BSA; 2, taking dimethyl sulfoxide as a solvent for dissolving glucan, carboxybenzaldehyde and 4-dimethylaminopyridine to form a solution, next, adding dicyclohexylcarbodiimide to the solution and then starting reaction, and performing after-treatment to obtain polyfunctional glucan; 3, adding a co-emulsifier, an emulsifier, the aminated BSA, a buffer solution and the polyfunctional glucan to a container, and then adding an oil phase, and oscillating to obtain the protein hydrogel microspheres. The method is simple in synthesis steps and convenient to operate; the microsphere size can be changed by changing the processing conditions, and consequently, the form of the material filled surface can be further optimized; and the method for preparing the protein hydrogel microspheres is very effective.

Description

technical field [0001] The invention belongs to the technical field of preparation of biological tissue medical materials, and relates to a method for obtaining protein hydrogel microspheres by taking bovine serum albumin as a base unit inverse microemulsion. Background technique [0002] Local implantation of tissue engineering biofilling materials is one of the effective methods for plastic and reconstructive surgery to treat related diseases (Ghoniem GM, Elgamasy AN, Elsergany R, et al. Grades of intrinsic spincteric deficiency (ISD) associated with female stress urinary incontinence[J] . Int Urogynecol J, 2002:13(2):99-105.). Filling materials are generally divided into two categories: autologous adipose tissue and biological materials. Although autologous tissue transplantation is the most important treatment method in plastic surgery, the autologous tissue is limited. For this reason, biomedical materials made according to different needs have been playing an importa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/38A61L27/22A61L27/52
Inventor 黄鑫文苹刘小曼吴光瑜周佩
Owner HARBIN INST OF TECH
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