Preparation method of chebulagic acid

A technology of Helile ellagic acid and ultrasound, which is applied in the field of preparation of Helile ellagic acid, can solve the problems of low extraction rate of plant active ingredients, unsuitability for industrial production, low product purity, etc., and achieve good regeneration performance , long service life and high separation efficiency

Inactive Publication Date: 2015-09-30
NANJING ZELANG MEDICAL TECH
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Problems solved by technology

The product obtained by this type of method has low purity, and the extraction rate of plant active ingredients is not high, so it is not suitable for industrial production

Method used

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  • Preparation method of chebulagic acid

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Experimental program
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Effect test

Embodiment 1

[0021] The leaves of Myrobalan are dried at 60°C, crushed, and passed through a 20-mesh sieve; the coarse powder of the medicinal material is put into a dilute acid solution with a pH value of 4 and a solid-to-liquid ratio of 1:0.5, and cellulose with a weight of 0.1% of the coarse powder is added to it. Enzyme and 0.1% pectinase were used for enzymolysis at 45°C for 1 hour; the obtained enzymolysis mixture was placed in an ultrasonic extractor, and a solid-liquid ratio of 1:2, 50% ethanol aqueous solution was added, and the frequency Ultrasonic extraction under the condition of 20KHz is carried out once, each time for 1 hour, to obtain the crude extract of ellagic acid from Chrysalis; The ceramic membrane separates the protein, polysaccharide and other substances in the crude extract; then put the obtained extract on the X-5 macroporous adsorption resin column, first elute with distilled water of 2 times the column volume, and then use 2 times the column volume , 40% ethanol ...

Embodiment 2

[0023] Take the leaves of myrobalan and dry them at 70°C, crush them, and pass through a 60-mesh sieve; put the above-mentioned coarse powder into a dilute acid solution with a pH value of 5 and a solid-to-liquid ratio of 1:1, and add 0.2% cellulose by weight of the coarse powder to it. Enzyme and 0.2% pectinase were used for enzymolysis at a temperature of 50°C for 2 hours; the obtained enzymolysis mixture was placed in an ultrasonic extractor, and the ratio of solid to liquid was 1:4, and 60% ethanol aqueous solution was added. 30KHz ultrasonic extraction twice, each time for 1.5 hours, to obtain the crude extract of ellagic acid; the crude extract of ellagic acid was first separated with a 0.4 micron ceramic membrane at a temperature of 30°C Proteins, polysaccharides and other substances in the crude extract; then put the obtained extract on the LSA-21 macroporous adsorption resin column, first elute with 3 times the column volume of distilled water, and then use 3 times the...

Embodiment 3

[0025] Take the leaves of myrobalan and dry them at 80°C, crush them, and pass through a 80-mesh sieve; put the above-mentioned coarse powder into a dilute acid solution with a pH value of 5 and a solid-to-liquid ratio of 1:1, and add 0.3% cellulose by weight of the coarse powder to it. Enzyme and 0.2% pectinase were used for enzymolysis at a temperature of 50°C for 2 hours; the obtained enzymolysis mixture was placed in an ultrasonic extractor, and a solid-liquid ratio of 1:5 and 70% ethanol aqueous solution were added. Ultrasonic extraction at 40KHz for 3 times, each time for 2 hours, to obtain the crude extract of ellagic acid; the crude extract of ellagic acid was first separated with a 0.4 micron ceramic membrane at a temperature of 40°C Remove the protein, polysaccharide and other substances in the crude extract; then put the obtained extract on the WLD macroporous adsorption resin column, first elute with 3 times the column volume of distilled water, and then use 4 times...

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Abstract

The invention discloses a preparation method of chebulagic acid, particularly a method for extracting chebulagic acid by ultrasonic-extraction-assisted bioenzyme enzymolysis. The method comprises the following steps: raw material treatment, enzymolysis, ultrasonic extraction, purification, vacuum concentration and freeze-drying to obtain the chebulagic acid. The method has the advantages of simple technique, mild reaction conditions, high selectivity, high chebulagic acid purity and the like.

Description

technical field [0001] The invention discloses a method for preparing ellagic acid from challis ellagic acid, which belongs to the field of biotechnology. Background technique [0002] Chebula ellagic acid, English name: Chebulagic acid, molecular formula C 41 h 30 o 27 , the molecular weight is 954.672, CAS registration number: 23094-71-5, mainly exists in various plants of Hematoxylinaceae, Gentlemaniaceae and Sapindaceae. Among them, the content of the leaves of the Junziceae plant Myrobalan is abundant. Its structural formula is as follows: [0003] [0004] Modern studies have proved that ellagic acid has strong antiviral activity, and it can also be used as a raw material for the synthesis of other active derivatives. [0005] It is recorded in "Compendium of Materia Medica" that the leaf of the Chinese medicine Myrobalan has the effects of lowering Qi and eliminating phlegm, quenching thirst and diarrhea. [0006] Ceramic membranes, also known as inorganic c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H13/08C07H1/08
Inventor 刘东锋杨成东
Owner NANJING ZELANG MEDICAL TECH
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