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Method for separating and purifying alpha-solanine and alpha-chaconine in potato flower

A technology for separation and purification of potatoes, applied in the field of phytochemistry, can solve the problems of difficult separation at normal pressure, no separation technology of high-purity α-solanine, and high similarity of chemical structure, and achieves low production cost, good separation effect and simple process. Effect

Inactive Publication Date: 2015-09-30
YUNNAN AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Due to the high chemical structure similarity between α-solanine (α-solanine) and α-chaconine (α-chaconine) (the hydrogen atom in the hydroxyl group on the third carbon position of solanidine in α-solanine is respectively replaced by D-glucose , L-rhamnose, L-rhamnose, the hydrogen atoms in the hydroxyl group on the third carbon position of solanidine in α-chaconine are replaced by D-glucose, L-rhamnose, D-glucose respectively) , Atmospheric pressure separation is not easy, so far there is no high-purity α-solanine (α-solanine) and α-chaconine (α-chaconine) separation technology

Method used

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  • Method for separating and purifying alpha-solanine and alpha-chaconine in potato flower
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  • Method for separating and purifying alpha-solanine and alpha-chaconine in potato flower

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Embodiment 1

[0041] A method for separating and purifying α-solanine and α-chaconine from potato flowers, the three steps of extraction, separation and purification are as follows:

[0042] 1) extract

[0043] Wash the collected potato flowers, dry them in a constant temperature oven at 60°C to constant weight, crush the dried samples into 40-100 mesh powder, and store them in a refrigerator at 4°C;

[0044]Accurately weigh 100.0g of potato flower powder and put it into a 1000ml Erlenmeyer flask, conduct ultrasonic extraction with ethanol-acetic acid with a volume ratio of 8:1, the ratio of solid to liquid is 1:8, the ultrasonic frequency is 55Hz, and the extraction time is 50min / time , after each extraction, replace the extraction with a new extract, repeat three times, after the extraction is completed, combine the extracts for suction filtration, take the filtrate, put the filtrate in a rotary evaporator to concentrate to 1 / 10 of the original volume, add concentrated ammonia water Adju...

Embodiment 2

[0053] A method for separating and purifying α-solanine and α-chaconine from potato flowers, the three steps of extraction, separation and purification are as follows:

[0054] 1) extract

[0055] Wash the collected potato flowers, dry them in a constant temperature oven at 60°C to constant weight, crush the dried samples into 40-100 mesh powder, and store them in a refrigerator at 4°C;

[0056] Accurately weigh 100.0g of potato flower powder and put it into a 1000ml conical flask, conduct ultrasonic extraction with ethanol-acetic acid with a volume ratio of 12:1, the ratio of solid to liquid is 1:12, the ultrasonic frequency is 55Hz, and the extraction time is 50min / time , after each extraction, replace the extraction with a new extract, repeat three times, after the extraction is complete, combine the extracts for suction filtration, take the filtrate, put the filtrate in a rotary evaporator to concentrate to 1 / 6 of the original volume, add concentrated ammonia water Adjust...

Embodiment 3

[0065] A method for separating and purifying α-solanine and α-chaconine from potato flowers, the three steps of extraction, separation and purification are as follows:

[0066] 1) extract

[0067] Wash the collected potato flowers, dry them in a constant temperature oven at 60°C to constant weight, crush the dried samples into 40-100 mesh powder, and store them in a refrigerator at 4°C;

[0068] Accurately weigh 100.0g of potato flower powder and put it into a 1000ml Erlenmeyer flask, conduct ultrasonic extraction with ethanol-acetic acid with a volume ratio of 10:1, the ratio of solid to liquid is 1:10, the ultrasonic frequency is 55Hz, and the extraction time is 50min / time , after each extraction, replace the extraction with a new extract, repeat three times, after the extraction is complete, combine the extracts for suction filtration, take the filtrate, put the filtrate in a rotary evaporator to concentrate to 1 / 8 of the original volume, add concentrated ammonia water Adj...

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Abstract

The invention relates to a method for separating and purifying alpha-solanine and alpha-chaconine in potato flower and belongs to the field of phytochemistry. The method comprises extraction, separation and purification. The extraction process comprises carrying out extraction on potato flower powder by ethanol-acetic acid as an extraction agent, fully precipitating potato glycoalkaloid, and collecting crude product of the naturally aired precipitates. The separation process utilizes a column chromatography separation method and comprises carrying out packing, sample loading and elution to respectively obtain eluates containing alpha-solanine and alpha-chaconine and carrying out water-bath distillation drying on the eluates by a rotary evaporation instrument. The purification process comprises carrying out crystallization on the dried alpha-solanine and alpha-chaconine, and carrying out re-crystallization twice to obtain alpha-solanine and alpha-chaconine pure products. The method realizes separation and purification of alpha-solanine and alpha-chaconine pure products, has good separation effects, has simple processes and a low production cost and does not damage a glucoside part.

Description

technical field [0001] The invention belongs to the field of phytochemistry, and in particular relates to a method for separating and purifying α-solanine and α-chaconine from potato flowers. Background technique [0002] Different parts of the potato plant contain a secondary metabolite produced during respiration—steroidal alkaloids, which are also called total glycoalkaloids (TGA for short), solanine, and solanum nigrum. Alkaloids or potato toxins, potato leaves, flowers, buds and fruits contain a large amount of glycoalkaloids, and the total glycoalkaloid content in flowers at the end of flowering is the highest, and the total glycoalkaloid content in flowers at the end of flowering of some varieties can reach 6410.9mg kg -1 FW. [0003] According to the steroidal core skeleton, the glycoalkaloids in potato belong to cholestane (C 27 ) alkaloids (cholestane alkaloids), in potato cultivars are mainly solanine (solanine) and chaconine (chaconine) two categories, account...

Claims

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Application Information

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IPC IPC(8): C07J71/00
CPCC07J71/0052
Inventor 黄红苹郭华春王琼范明达陈万登
Owner YUNNAN AGRICULTURAL UNIVERSITY
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