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Preparation method for double-skin forward permeable membrane with aquaporin

A technology of aquaporin and forward osmosis membrane, which is applied in the field of preparation of double-cortex forward osmosis membrane containing aquaporin, can solve the problems of reducing the effective water flux of the membrane and reducing the effective driving pressure, so as to improve the effective water flux and broaden the Application prospect, effect of reducing inner concentration polarization

Inactive Publication Date: 2015-11-04
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the internal concentration polarization that occurs in the support layer of the forward osmosis membrane will lead to a significant reduction in the effective driving pressure, thereby significantly reducing the effective water flux of the membrane.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Mix 2.4 ml of 1 mg / ml 1,2-dioleoylphosphatidylcholine with 0.6 ml of 1,2-dioleyloxy-3-trimethylaminopropane in chloroform, blow dry with nitrogen and vacuum dry, then Add 30ml of phosphate buffer solution with pH=7.5, shake and sonicate to prepare the mixed phospholipid vesicle solution of the above two phospholipids; add aquaporin 1 (0.014mg / ml / ) / glycerol (0.1mg / ml) / n - 4.28ml of phosphate buffer solution of octyl-β-D-glucopyranoside (0.01mg / ml), put the mixed solution into a dialysis bag with a molecular weight cut-off of 7KDa, and then dialyze in phosphate buffer solution for 3 days to prepare Proteoliposomes with a ratio of protein to phospholipids of 1:50; the polyacrylonitrile membrane prepared by the phase inversion method was immersed in 1.5M NaOH solution for 1.5h, washed with deionized water, and 1g / L polyethyleneimine The solution and the sodium polystyrene sulfonate solution were alternately spread on the upper and lower surfaces of the basement membrane, an...

Embodiment 2

[0026] Mix 2.4ml of 1mg / ml 1-hexadecanoyl-SN-glycerol-phosphorylcholine with 0.6ml of 1,2-dioleyloxy-3-trimethylaminopropane in chloroform, blow dry with nitrogen and Vacuum-dry, then add 30ml of phosphate buffer solution with pH=7.5, and prepare the mixed phospholipid vesicle solution of the above two phospholipids through shaking and ultrasonication; add aquaporin 1 (0.015mg / ml / ) / glycerol (0.1mg / ml / ml) / n-octyl-β-D-glucopyranoside (0.01mg / ml) phosphate buffer solution 4.28ml, the mixed solution is packed into a dialysis bag with a molecular weight cut-off of 7KDa, and then dialyzed in the phosphate buffer solution After 3 days, prepare proteoliposomes with a ratio of protein to phospholipid of 1:50; immerse the sulfonated polysulfone membrane prepared by the phase inversion method in 1.5M NaOH solution for 1.5h, wash it with deionized water, and add 1g / L The polyethyleneimine solution and sodium polystyrene sulfonate solution were alternately spread on the upper and lower su...

Embodiment 3

[0028] Mix 2.4ml of 1mg / ml distearoylphosphatidylcholine with 0.6ml of 1,2-dioleyloxy-3-trimethylaminopropane in chloroform, blow dry with nitrogen and vacuum dry, then add pH= 30ml of phosphate buffer solution in 7.5, vibrate and sonicate to prepare the mixed phospholipid vesicle solution of the above two phospholipids; add aquaporin 1 (0.008mg / ml / ) / glycerol (0.1mg / ml) / n-octyl - 5.28ml of phosphate buffer solution of β-D-glucopyranoside (0.01mg / ml), put the mixed solution into a dialysis bag with a molecular weight cut-off of 7KDa, and then dialyze in phosphate buffer solution for 3 days to prepare protein and phospholipid Proteoliposomes with a ratio of 1:50; the polyacrylonitrile membrane prepared by the phase inversion method was immersed in 1.5M NaOH solution for 1.5h, washed with deionized water, and 1g / L polyethyleneimine solution was mixed with poly The sodium styrene sulfonate solution was alternately spread to the upper and lower surfaces of the basement membrane, an...

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Abstract

The invention relates to a preparation method for a double-skin forward permeable membrane with aquaporin. The preparation method specifically comprises the following steps of: 1) preparing a base membrane; 2) preparing a layer-by-layer assembly composite membrane; 3) preparing phospholipid vesicle solution; 4) introducing aquaporin; and 5) preparing a biomimic film with aquaporin phospholipid, wherein proteoliposome with aquaporin prepared in the step 4 is spread to the surface of the composite film prepared in the step 2, and then a cross-linking agent is used to carry out cross-linking to complete the preparation. According to the preparation method, the double-skin forward permeable membrane with aquaporin is prepared by layer-by-layer assembly; the double-skin forward permeable membrane can effectively prevent solute from entering the composite membrane, thereby reducing inner concentration polarization effect, and obviously improving effective water flux of the forward permeable membrane; and the the double-skin forward permeable membrane can be applied to different fields such as water treatment, membrane separation and the like, and has a wide application prospect.

Description

technical field [0001] The invention is a method for preparing a double-cortex forward osmosis membrane containing an aquaporin, specifically, a porous membrane layer-by-layer self-assembled polyelectrolyte layer is used as a base membrane, and a membrane containing aquaporin phospholipids is respectively cross-linked on the surface and bottom of the base membrane as a cortex. Thus, a forward osmosis composite membrane with high water permeability and ion interception capacity is prepared. Background technique [0002] Desalination of seawater is one of the important ways to solve the shortage of water resources in the world today. In recent years, membrane separation technology has developed rapidly in the field of water treatment by virtue of its advantages of energy saving, high efficiency and environmental protection. The key to membrane water treatment is the performance of the separation membrane. Developing new composite membrane materials to reduce energy consumpti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D69/12B01D71/82B01D67/00
Inventor 王志宁王树正王淼琪丁万德于致源闫艺心
Owner OCEAN UNIV OF CHINA
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