Method of preventing and treating parasitic weed orobanche coerulescens
A technology that can be classified and biodegradable, applied in the fields of botanical equipment and methods, chemicals used for biological control, biocides, etc. Low cracking strength and other problems, to achieve the effect of low comprehensive use cost, complete degradation, uniform and thorough degradation
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Embodiment 1
[0046] (1) Preparation of biocontrol bacteria powder
[0047] A biocontrol fungus Pythium melon and fruit with strong pathogenicity to Liedang was isolated from the naturally dead Liedang plants in Xinjiang. The Pythium melon fruit preserved on the slant was activated on the PDA plate, and the mycelium was collected after the bacterial block was shaken in the PD medium. The collected mycelia of Pythium melon and fruit were placed in 1000 mL of liquid NA medium, and vibrated at a temperature of 28-30° C. and a rotational speed of 180 r / min for 72 hours to obtain a Pythium melon fruit fermentation broth. Centrifuge the fermentation liquid of Pythium melon and fruit at 3600r / min for 30min at room temperature, take the supernatant, mix it with 30g of macroporous resin AmberliteXAD16, shake at 28°C and 160r / min for 4h, and then fill the column. Wash the column with distilled water first, then elute with acetone, and collect the acetone eluate. Concentrate the acetone eluate by ro...
Embodiment 2
[0056] Step (1) preparation of biocontrol bacteria powder and step (2) preparation of polyester biodegradation raw material are the same as in Example 1.
[0057] Step (3) preparation of biodegradable mulch film:
[0058] Increase the biocontrol bacterium powder that contains 4%PVP in the auxiliary agent pipeline 7, wherein biocontrol bacterium powder 3.0Kg, other as the step (3) of embodiment 1;
[0059] 4) The control effect of Liedang
[0060] The polyester biodegradable mulch film prepared above for preventing and controlling parasitic weeds, and common mulch were respectively laid in the fields adjacent to melons and radang; Effects of polyester biodegradable mulch and common mulch on yield of melon. The results showed that there was no significant difference in growth and yield between two melons covered with different plastic films, and the melons were basically not affected. In the second year, the effect of the polyester biodegradable mulch film on the parasitic we...
Embodiment 3
[0062] Step (1) preparation of biocontrol bacteria powder and step (2) preparation of polyester biodegradation raw material are the same as in Example 1.
[0063] Step (3) preparation of biodegradable plastic film
[0064] Increase biocontrol bacterium powder and humic acid containing 4%PVP in the auxiliary agent pipeline 7, wherein each 3.0Kg of biocontrol bacterium powder and humic acid, other as the step (3) of embodiment 1;
[0065](4) The control effect of Liedang
[0066] The above-mentioned polyester biodegradable mulch film for preventing and controlling parasitic weeds, and common mulch were laid separately in the fields with melons and radon; Effects of biodegradable and common mulches on yield of melons. The results showed that there was no significant difference in growth and yield between two melons covered with different plastic films, and the melons were basically not affected. In the second year, the effect of the polyester biodegradable mulch film on the pa...
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