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Silk fibroin microspheres loaded with arbutin and vitamin C, preparation method and application thereof

A silk fibroin microsphere and silk fibroin technology, applied in the field of protein microsphere preparation, can solve the problems of low transdermal absorption rate, insignificant whitening effect, and inability to directly apply arbutin and vitamin C to the skin, etc. Achieve the effect of promoting metabolism, increasing transdermal absorption, and solving poor transdermal absorption

Active Publication Date: 2017-12-19
西安艾尔菲生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a silk fibroin microsphere loaded with arbutin and vitamin C, which solves the problem that arbutin and vitamin C cannot be directly applied to the skin in the prior art
[0006] The third object of the present invention is to provide the application of the above-mentioned silk fibroin microspheres loaded with arbutin and vitamin C, which solves the problem of the low transdermal absorption rate of arbutin and vitamin C in the existing whitening cosmetics and the insignificant whitening effect The problem

Method used

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  • Silk fibroin microspheres loaded with arbutin and vitamin C, preparation method and application thereof
  • Silk fibroin microspheres loaded with arbutin and vitamin C, preparation method and application thereof
  • Silk fibroin microspheres loaded with arbutin and vitamin C, preparation method and application thereof

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preparation example Construction

[0037] The present invention also provides a method for preparing the aforementioned silk fibroin microspheres loaded with arbutin and vitamin C, comprising the following steps:

[0038] Step 1, prepare silk fibroin:

[0039]Step 1-1, according to the mass ratio of silk and 0.01~0.1mol / L sodium carbonate solution is 1:10~1:5, add silk into the sodium carbonate solution of 100 ℃, heat for 20~30min and then put Boil in deionized water at 90-100°C for 30 minutes, and vacuum dry the boiled silk at 35°C for 20 minutes to obtain degummed silk;

[0040] Step 1-2, add degummed silk to the ternary system CaCl 2 :C 2 H 5 OH:H 2 O, incubate at 70-80 °C for 2 h, and then at a centrifugal force of 1.35 × 10 4 Centrifuge at g for 8-10 min to obtain a silk fibroin solution;

[0041] Step 1-3, put the silk fibroin solution into a cellulose dialysis tube and dialyze it in deionized water at 4 °C for 7 days. After the dialyzed silk fibroin solution is pre-frozen at -80 °C for 15 hours, it...

Embodiment 1

[0056] In the present embodiment, a silk fibroin microsphere containing arbutin and vitamin C is prepared, and the preparation method is as follows:

[0057] Step 1, Preparation of Silk Fibroin

[0058] Step 1-1, add 30.00g of silk into 100mL of 0.02mol / L sodium carbonate solution at 100°C, heat it for 25min, then boil it in deionized water at 95°C for 30min, and vacuum dry the boiled silk at 35°C for 20min, Obtain degummed silk.

[0059] Step 1-2, add degummed silk to the ternary system CaCl 2 :C 2 H 5 OH:H 2 O, CaCl 2 , C 2 H 5 OH, H 2 The molar ratio of O was 1:2:8, and the temperature was kept at 75 °C for 2 h, and then the centrifugal force was 1.35 × 10 4 Centrifuge at g for 9 min to obtain a silk fibroin solution.

[0060] Steps 1-3, put the silk fibroin solution into a cellulose dialysis tube and dialyze it in deionized water at 4 °C for 7 days. After the dialyzed silk fibroin solution is pre-frozen at -80 °C for 15 hours, it is placed in a vacuum with a vacu...

Embodiment 2

[0075] In the present embodiment, a silk fibroin microsphere containing arbutin and vitamin C is prepared, and the preparation method is as follows:

[0076] Step 1, Preparation of Silk Fibroin

[0077] Step 1-1, add 50.00g of silk into 500mL of 0.1mol / L sodium carbonate solution at 100°C, heat for 30min, then boil in deionized water at 100°C for 30min, and vacuum dry the boiled silk at 35°C for 20min, Obtain degummed silk.

[0078] Step 1-2, add degummed silk to the ternary system CaCl 2 :C 2 H 5 OH:H 2 O, CaCl 2 , C 2 H 5 OH, H 2 The molar ratio of O was 1:2:8, and the temperature was kept at 80 °C for 2 h, and then the centrifugal force was 1.35 × 10 4 Centrifuge at g for 10 min to obtain a silk fibroin solution.

[0079] Steps 1-3, put the silk fibroin solution into a cellulose dialysis tube and dialyze it in deionized water at 4 °C for 7 days. After the dialyzed silk fibroin solution is pre-frozen at -80 °C for 15 hours, it is placed in a vacuum with a vacuum de...

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Abstract

The invention discloses a silk fibroin microsphere carrying arbutin and vitamin C, which is composed of the following raw material components: 20%-60% silk fibroin, 2%-8% arbutin, and 10% vitamin C ~20%, lyoprotectant 20%~60%, distilled water 1%~4%, the sum of the above total scores is 100%. The present invention encapsulates arbutin and vitamin C in silk fibroin and prepares them into nano-scale microspheres, which can effectively solve the problem of poor transdermal absorption capacity of arbutin and vitamin C, and increase its transdermal absorption Performance, after the skin absorbs the silk fibroin microspheres, the silk fibroin microspheres loaded with arbutin and vitamin C can slowly release the active ingredients in the skin to achieve long-term whitening effect. The silk fibroin microspheres carrying arbutin and vitamin C of the present invention can be added to cosmetics to prepare cosmetics with whitening effect.

Description

technical field [0001] The invention belongs to the technical field of protein microsphere preparation, relates to a silk fibroin microsphere loaded with arbutin and vitamin C, and also relates to a preparation method and application of the silk fibroin microsphere. Background technique [0002] Whitening has always been a matter of great concern to beauty lovers, and it is a constant theme of cosmetics. Arbutin and vitamin C are recognized as raw materials with whitening and anti-aging effects. Inhibit the activity of tyrosinase in the skin, block the formation of melanin, and accelerate the decomposition and excretion of melanin by directly combining itself with tyrosinase, thereby reducing skin pigmentation, removing spots and freckles, and slowing down skin aging. Moreover, it does not produce toxic, irritating, sensitizing and other side effects on melanocytes, and also has bactericidal and anti-inflammatory effects. Chinese patent "A Skin Whitening Composition" (appli...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/67A61K8/60A61K8/64A61K8/02A61Q19/02A61Q19/08
Inventor 赵文静王静胡春梅姚莎莎
Owner 西安艾尔菲生物科技有限公司
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