Compound liver-protecting traditional Chinese medicine for treating non-alcoholic fatty liver disease (NAFLD)

A non-alcoholic and fatty liver technology, which is applied in the field of compound liver-protecting traditional Chinese medicine for the treatment of non-alcoholic fatty liver, can solve the problems of reducing absorption and increasing carotenoid absorption, and achieves the reduction of hepatic steatosis, no cytotoxicity, and reduced The effect of TG content in the liver

Inactive Publication Date: 2015-11-11
QINGDAO MUNICIPAL HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A high-cholesterol diet may increase carotenoid absorption whereas a low-fat diet decreases its absorption

Method used

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  • Compound liver-protecting traditional Chinese medicine for treating non-alcoholic fatty liver disease (NAFLD)
  • Compound liver-protecting traditional Chinese medicine for treating non-alcoholic fatty liver disease (NAFLD)
  • Compound liver-protecting traditional Chinese medicine for treating non-alcoholic fatty liver disease (NAFLD)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1, In Vitro Experiment of the Compound Liver-Protecting Traditional Chinese Medicine in Treating Nonalcoholic Fatty Liver

[0040] 1. Liquid preparation

[0041] (1) Preparation of 1% (w / w) PBS buffer

[0042] Weigh 2.0g of PBS powder into a 250mL jar, add 200mL of double-distilled water to dissolve, sterilize by high-pressure steam, and store in an ordinary refrigerator at 4°C.

[0043] (2) Preparation of cell culture medium (10% of fetal bovine serum and 1% of penicillin and streptomycin)

[0044] In the ultra-clean bench, add 15 mL of fetal bovine serum to a jar (250 mL), then add 1.5 mL of double antibody (penicillin and streptomycin), and finally add an appropriate amount of RPMI-1640 culture solution to make the volume to 150 mL, filter and sterilize, Store in a normal refrigerator at 4°C.

[0045] (3) Preparation of cell cryopreservation solution

[0046] Add 7mL of RPMI-1640 culture medium, 2mL of fetal bovine serum and 1mL of DMSO into a centrifuge ...

Embodiment 2

[0098] Example 2, In Vivo Experiment of the Compound Liver-Protecting Traditional Chinese Medicine in Treating Non-alcoholic Fatty Liver

[0099] 1. Experimental materials

[0100] 1.1 Experimental animals

[0101] C57BL / 6J male mice were purchased from Beijing Weitong Lihua Company, SPF grade, weighing 18-22 g. The compound liver-protecting traditional Chinese medicine was purchased from Dalian Zhongke Geleike Biotechnology Co., Ltd. The experimental animals were kept under SPF standard laboratory conditions, kept well ventilated, the ambient temperature was controlled at 23°C±2°C, the humidity was controlled at 40%-50%, 12h light / 12h dark cycle, and free intake of food and water. The mice were fed normally for 1 week before the start of the study, and the experiment started from the 2nd week.

[0102] 1.2 Feed formula

[0103] 10% of the energy in the basic feed comes from fat, 70% from carbohydrates, and 20% from protein. 45% of the energy in a high-fat diet comes from f...

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Abstract

The invention provides a compound liver-protecting traditional Chinese medicine for treating non-alcoholic fatty liver disease (NAFLD), which comprises the following components in parts by weight: 2-10 parts of chitosan oligosaccharide, 3-20 parts of salidroside, 5-10 parts of resveratrol, 10-20 parts of astaxanthin and 5-15 parts of stichopus japomicus slelenka glycosaminoglycans. According to the invention, through NAFLD mouse model experiments in vivo and vitro, five bioactive ingredients of the salidroside, chitosan oligosaccharide, resveratrol, astaxanthin and stichopus japomicus slelenka glycosaminoglycans are determined by a cell and molecular biology method, and after the bioactive ingredients are scientifically proportioned, lipid metabolism related genes can be adjusted, the effect of reducing the content of triglyceride in NAFLD liver cells is obvious, cell apoptosis induced by fatty acid can be relieved, hepatic steatosis of high-fat diet mice can be relieved, the TG content in the liver can be reduced, the medicine does not have obvious side effects and has no cytotoxicity, and the immune balance of the organism can not be damaged.

Description

technical field [0001] The invention belongs to the technical field of medicines, and in particular relates to a compound liver-protecting traditional Chinese medicine for treating non-alcoholic fatty liver. technical background [0002] Nonalcoholic fatty liver disease (NAFLD) is a clinicopathological syndrome characterized by fatty degeneration, necrosis, inflammatory cell infiltration, and fat accumulation in hepatic parenchymal cells. Its disease spectrum includes: simple fatty liver ( NAFL), steatohepatitis (NASH) and related cirrhosis. Nonalcoholic fatty liver disease has become the leading cause of chronic liver disease in western developed countries. In my country, NAFLD is the second most common liver disease after chronic viral hepatitis. The pathogenesis of NAFLD is very complex, mostly caused by factors such as obesity, diabetes, hyperlipidemia, insulin resistance and metabolic disorder syndrome affecting liver fat metabolism. NAFLD can coexist with other chro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/737A61P1/16A61K31/7032A61K31/702A61K31/722A61K31/122A61K31/05
Inventor 辛永宁隋牮箐栾桂萍林中华
Owner QINGDAO MUNICIPAL HOSPITAL
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