Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of calcium phosphate nanoparticles embedding cisplatin and its preparation method and application

A nanoparticle and calcium phosphate technology, which is applied in the field of preparation of calcium phosphate nanoparticles, can solve the problem of reducing the killing effect of tumor cells, and achieve the effects of controllable synthesis method, good biocompatibility, and mild preparation conditions

Active Publication Date: 2017-12-26
黄山市开发投资集团有限公司
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Although calcium phosphate has so many advantages, there are relatively few studies on calcium phosphate nanoparticles embedding cisplatin in recent years, and in the existing studies, cisplatin is generally dispersed on the surface of calcium phosphate.
In this way, before reaching the tumor tissue, cisplatin will be released during the transport in the body, thereby reducing the killing effect on tumor cells

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of calcium phosphate nanoparticles embedding cisplatin and its preparation method and application
  • A kind of calcium phosphate nanoparticles embedding cisplatin and its preparation method and application
  • A kind of calcium phosphate nanoparticles embedding cisplatin and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Embodiment 1, the synthesis and characterization of hydrophilic block copolymer PEG-PAA

[0056] 1. Synthesis of hydrophilic copolymer PEG-PAA

[0057] The hydrophilic block copolymer PEG-PAA is made of ETP-PEG 5K It is obtained by polymerization under the catalytic conditions of AIBN.

[0058] 1. Synthesis of reversible addition-fragmentation chain transfer polymerization reagent (ETP)

[0059] The synthetic route of reversible addition-fragmentation chain transfer polymerization reagent (ETP) is as follows figure 1 Shown: ethanethiol (24.852g, 400mmol) and 60mL of distilled water are mixed and stirred, then slowly dropwise add 32g of a mass concentration of 50% NaOH solution (wherein NaOH 16g, 400mmol) in an ice bath, and then Add 20mL of acetone under the condition and stir for 30min. Then carbon disulfide (34.2 g, 450 mmol) was added and the solution turned into a clear orange, and stirred for 30 min. 2-Bromopropionic acid (62.73 g, 410 mmol) was slowly added d...

Embodiment 2

[0074] Example 2, Preparation and Characterization of Calcium Phosphate Nanoparticles Encapsulating Cisplatin

[0075] 1. Preparation of cisplatin-encapsulated calcium phosphate nanoparticles (CaP NP / CDDP)

[0076] Amphiphilic block copolymers have been extensively studied as drug delivery vehicles. When amphiphilic block copolymers are dissolved in a good solvent for one block and a poor solvent for the other block, the copolymers spontaneously assemble into aggregates.

[0077] However, the method of forming micelles with hydrophilic block copolymers is rarely used. In this description, part of the carboxyl group of the hydrophilic block copolymer PEG-PAA is coordinated with cisplatin to connect the cisplatin to the copolymer, and then use the method of calcium ion micelle nucleation to make the hydrophilic block copolymer Micelles are formed, and finally the micelles are combined with hydrogen phosphate to form a calcium phosphate mineral layer, so that cisplatin can be e...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
particle diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a calcium phosphate nanoparticle embedding cisplatin and its preparation method and application. Ether reacts to generate ETP-PEG5K, and then ETP-PEG5K is catalyzed by azobisisobutyronitrile to polymerize monomer acrylic acid to obtain a hydrophilic block copolymer PEG-PAA; some carboxyl groups of PEG-PAA react with cisplatin Coordination combination, so that cisplatin is attached to PEG-PAA, and then use calcium ion micelle nucleation method to make PEG-PAA form micelles, and finally the micelles are combined with hydrogen phosphate to form a calcium phosphate mineral layer, so that cisplatin Embedded by the calcium phosphate layer, the calcium phosphate nanoparticles embedded with cisplatin are obtained. The product of the invention has good biocompatibility, particle stability and pH responsiveness.

Description

technical field [0001] The invention belongs to the technical field of nano drug carriers, and relates to a preparation method and application of calcium phosphate nanoparticles embedded with cisplatin. Background technique [0002] Cancer is a disease with a high mortality rate in the world, and chemotherapy is one of its main treatment methods. Cisplatin (Cisplatin) is an important anti-tumor agent, it is widely used in the clinical treatment of many malignant tumors, such as testicular cancer, ovarian cancer, bladder cancer, head and neck squamous cell carcinoma, small cell and non-small cell lung cancer, etc. . However, with the clinical use of cisplatin more and more widely, clinical drug resistance is also gradually highlighted, and cisplatin as a better chemotherapy drug gradually shows its limitations. Moreover, cisplatin also has many toxic and side effects in the process of clinical use, such as nephrotoxicity, neurotoxicity, and decreased perception. [0003] I...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/02A61K47/59A61K33/24A61P35/00
Inventor 陶伟董云雷姚日生杨显珠马寅初丁洋
Owner 黄山市开发投资集团有限公司