Trametes sp. and application thereof

A technology of Trametes and laccase, applied in Trametes and its application fields, can solve the problems of long fermentation time, high fermentation cost, low fermentation activity, etc.

Active Publication Date: 2015-11-11
ANHUI UNIVERSITY OF TECHNOLOGY AND SCIENCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although laccase has been industrially produced and applied, the laccase-producing strains currently used in research or production still have the disadvantages of long fermentation time and low fermentation activity, which lead to high fermentation costs and restrict the wide application of laccase.

Method used

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  • Trametes sp. and application thereof
  • Trametes sp. and application thereof
  • Trametes sp. and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Take the ultra-low temperature (-80°C) glycerol freezing tube of the preserved Trametes trichotillobacter strain and dissolve it at room temperature, draw 0.1mL liquid to coat the PDA plate, incubate at 30°C for 5 days, wash the mycelium with sterile water, and use 5mL sterile water for each plate. Wash with water, absorb 2 mL of the mycelia suspension obtained after washing and insert it into 48 mL of sterile seed culture medium. Culture conditions: the culture temperature is 30°C, the rotation speed of the shaker is 220rpm, and after 48 hours of constant temperature cultivation, it becomes the seed culture solution for shaking flask fermentation to produce enzymes.

[0057] Put 45mL of enzyme-producing medium in a 250mL Erlenmeyer flask, insert 5mL of seed culture solution, and place in a shaking constant temperature culture for cultivation. Culture conditions: the culture temperature is 30° C., and the rotation speed of the shaker is 220 rpm. Under the above culture...

Embodiment 2

[0060] The seed culture liquid of shaking flask fermentation to produce enzyme is the same as embodiment 1

[0061] Put 45mL of enzyme-producing medium in a 250mL Erlenmeyer flask, insert 5mL of seed culture solution, and place in a shaking constant temperature culture for cultivation. Culture conditions: the culture temperature is 30° C., and the rotation speed of the shaker is 220 rpm. Under the above culture conditions, after 8 days of culture, the laccase activity in the fermentation broth was 304U / mL.

[0062] Each L of enzyme production medium contains: glucose 10g, 10% bran extract juice 100g, soybean meal 7g, yeast powder 2.5g, copper sulfate pentahydrate 0.25g, sodium chloride 1g, potassium dihydrogen phosphate 1g, calcium chloride 0.3g , pH5.5.

Embodiment 3

[0064] The seed culture liquid of shaking flask fermentation to produce enzyme is the same as embodiment 1

[0065] Put 45mL of enzyme-producing medium in a 250mL Erlenmeyer flask, insert 5mL of seed culture solution, and place in a shaking constant temperature culture for cultivation. Culture conditions: the culture temperature is 30° C., and the rotation speed of the shaker is 220 rpm. Under the above culture conditions, after 8 days of culture, the laccase activity in the fermentation broth was 326U / mL.

[0066] Each L of enzyme production medium contains: glucose 20g, 10% bran extract juice 120g, ammonium chloride 10g, yeast powder 3g, copper sulfate pentahydrate 0.25g, sodium chloride 1g, potassium dihydrogen phosphate 1g, calcium chloride 0.3 g, pH5.5.

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PUM

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Abstract

The invention relates to a Trametes sp. and an application thereof. The above strain has the advantages of rapid growth, wide utilization range of a nutrition matrix, cheap and easily available medium components, and easy culturing. Laccase produced by using the strain can be used in the fields of biomass energy, texture, foods, biological restoration and papermaking.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to Trametes and its application. Background technique [0002] Microorganisms of the genus Trametes, white-rot fungi belonging to the phylum Basidiomycota. White rot fungi rely on the ability to degrade lignin-cellulose materials to penetrate wood, invade the wood cell cavity, and release degradative enzymes, causing wood to rot and turn white. It is the microorganism with the strongest ability to degrade aromatic compounds. White-rot fungi mainly use the secreted peroxidases (LiPs, MnPs and laccase) to catalyze the oxidation reaction of lignin, thereby degrading lignin molecules. Compared with the other two lignin-degrading enzymes LiPs and MnPs, laccase is the only lignin-degrading enzyme that has achieved industrial production and application. [0003] Laccases are widespread in wood-rotting fungi and are also found in species such as plants, fungi, bacteria, and insects. Studies ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N9/02C12R1/645
Inventor 李松汤斌
Owner ANHUI UNIVERSITY OF TECHNOLOGY AND SCIENCE
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