Analytical methods for organic carbon bioactivity

A technology of biological activity and analysis method, applied in the field of molecular detection, can solve the problems of restricting the understanding of marine carbon pool and marine carbon storage, and the lack of quantitative analysis methods for organic carbon, etc., to achieve easy operation, high accuracy and good repeatability Effect

Active Publication Date: 2018-04-10
XIAMEN UNIV
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  • Description
  • Claims
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Problems solved by technology

[0005] However, there has been a lack of quantitative analysis methods for the biological activity of organic carbon for a long time, which greatly restricts people's understanding of the ocean carbon pool and the process and mechanism of ocean carbon storage.

Method used

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  • Analytical methods for organic carbon bioactivity
  • Analytical methods for organic carbon bioactivity
  • Analytical methods for organic carbon bioactivity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The analysis of embodiment 1 amino acid bioactivity

[0025] 1.1 Preparation of microactivity calorimeter

[0026] Analysis of microbial metabolic activity was performed in a microactivity calorimeter. In this embodiment, the temperature is set at 28° C., and the instrument is stabilized for 1 to 2 hours.

[0027] 1.2 Preparation of organic carbon stock solution

[0028] Preparation of organic carbon stock solution: In this example, the biological activities of organic carbon L-glutamic acid, D-glutamic acid, L-valine and D-valine were analyzed. The concentration of the organic carbon stock solution was 10 mM, and the pH was adjusted to 7.0. The stock solution was sterilized by filtration through a 0.22 μm sterilized microporous membrane.

[0029] 1.3 Pre-cultivation of bacteria

[0030] The composition of medium M1 used in this example is 1L containing Bacto Peptone, 1.0g; yeast extract, 1.0g; KCl, 0.3g; MgSO 4 ·7H 2 O, 0.5g; CaCl 2 2H 2 O, 0.038g; NH 4 Cl, 0...

Embodiment 2

[0039] The analysis of the biological activity of embodiment 2 sugar, alcohol and organic acid

[0040] 2.1 Preparation of the microactivity calorimeter

[0041] After setting the temperature to the culture temperature of 28°C, stabilize the instrument for 1-2 hours.

[0042] 2.2 Preparation of organic carbon stock solution

[0043] Preparation of organic carbon stock solution: In this example, the biological activity of glucose, acetic acid, glycerol, and glucosamine on the marine bacterium Dinoroseobacter shibae DFL12 (DSM16493) was analyzed. The concentration of the organic carbon stock solution was 10%, and the pH was adjusted to 7.0. The stock solution was sterilized by filtration through a 0.22 μm sterilized microporous membrane.

[0044] 2.3 Pre-cultivation of bacteria

[0045] In this example, the composition of medium M1 is 1L containing Bacto Peptone, 1.0g; yeast extract, 1.0g; NaCl 20g, KCl 0.3g,, MgSO 4 ·7H 2 O 0.5g, CaCl 2 2H 2 O 0.05g, NH 4 Cl 0.3g, K 2...

Embodiment 3

[0054] The analysis of embodiment 3 sugar and amino acid biological activity

[0055] 3.1 Preparation of the microactivity calorimeter

[0056] Analysis of microbial metabolic activity was performed in a microactivity calorimeter. In this embodiment, the temperature is set at 28° C., and the instrument is stabilized for 1 to 2 hours.

[0057] 3.2 Preparation of organic carbon stock solution

[0058] In this example, the biological activities of organic carbon glucose, D-alanine, D-glutamic acid, D-methionine and D-leucine were analyzed. The organic carbon stock solution was 10 mM and the pH was adjusted to 7.0. The stock solution was sterilized by filtration through a 0.22 μm sterilized microporous membrane.

[0059] 3.3 Pre-cultivation of bacteria

[0060] In this example, the components (L) of medium M1 are: Bacto Peptone, 1.0g; yeast extract, 1.0g; KCl, 0.3g; MgSO 4 ·7H 2 O, 0.5g; CaCl 2 2H 2 O, 0.038g; NH 4 Cl, 0.3g; K2HPO4, 0.3g; and NaCl, 20.0g; 1mL trace eleme...

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Abstract

Analytical method for organic carbon bioactivity involving molecular detection. Provides an analytical method for the real-time quantitative analysis of organic carbon bioactivity against marine bacterial bioactivity. 1) preparing the organic carbon into an aqueous solution to obtain an organic carbon stock solution; 2) filtering and sterilizing the organic carbon stock solution obtained in step 1). 3) Inoculate the bacteria to be analyzed into medium M1 for pre-culture, so that the turbidity of the bacteria reaches OD600=1, centrifuge the bacteria liquid, remove the supernatant, collect the bacteria, add NaCl to the bacteria, and suspend Centrifuge again, suspend the cells with NaCl to obtain the pre-culture solution of the cells; 4) put the stock solution M2 into a culture bottle, then add sterilized distilled water, organic carbon stock solution, and the pre-culture solution of the cells, and prepare the culture bottle Cover it, put it into a micro-activity calorimeter for cultivation, record the heat change in the cultivation process, and analyze the bioactivity of organic carbon. High sensitivity, real-time detection, easy operation, good repeatability and high accuracy.

Description

technical field [0001] The invention relates to the technical field of molecular detection, in particular to an analysis method for biological activity of organic carbon. Background technique [0002] The ocean covers 71% of the Earth's surface, and about one-third of the carbon dioxide produced by known human activities is absorbed by the ocean. The ocean carbon cycle has a major impact on global climate change. [0003] Organic carbon in the ocean is mainly in the form of dissolved organic carbon. About 95% of dissolved organic carbon is inert organic carbon that is difficult to use for biology (Eichinger et al., 2006; Hansell et al., 2009), which can be stored in the ocean for thousands of years, and its total amount can be compared with atmospheric carbon dioxide comparable to the total carbon that constitutes ocean carbon storage. [0004] Marine micro organisms are the main source of marine inert organic carbon. Micro organisms convert active organic carbon into iner...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/02G01N25/20
Inventor 张子莲焦念志王蕊
Owner XIAMEN UNIV
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